Manganese in PDB 2feu: P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX

All present enzymatic activity of P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX:
1.14.15.1;

The structure of P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX, PDB code: 2feu was solved by K.Von Koenig, T.M.Makris, S.G.Sligar, I.Schlichting, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.80 / 1.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	67.130, 62.230, 94.710, 90.00, 90.64, 90.00
R / Rfree (%)	21.4 / 23.8

The structure of P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX also contains other interesting chemical elements:

Potassium

(K)

3 atoms

The binding sites of Manganese atom in the P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX (pdb code 2feu). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX, PDB code: 2feu:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn730 b:15.7 occ:1.00 MN A:MNR730 0.0 15.7 1.0 NA A:MNR730 2.0 17.0 1.0 NB A:MNR730 2.0 18.6 1.0 ND A:MNR730 2.0 16.0 1.0 NC A:MNR730 2.0 17.4 1.0 SG A:CYS357 2.4 18.1 1.0 C1A A:MNR730 3.0 18.8 1.0 C4B A:MNR730 3.1 19.8 1.0 C1C A:MNR730 3.1 17.4 1.0 C4A A:MNR730 3.1 20.5 1.0 C4D A:MNR730 3.1 18.5 1.0 C1B A:MNR730 3.1 18.8 1.0 C1D A:MNR730 3.1 18.0 1.0 C4C A:MNR730 3.1 18.2 1.0 CB A:CYS357 3.4 16.0 1.0 CHC A:MNR730 3.4 19.1 1.0 CHA A:MNR730 3.4 19.6 1.0 CHB A:MNR730 3.4 19.5 1.0 CHD A:MNR730 3.5 18.1 1.0 CA A:CYS357 4.0 17.8 1.0 C5 A:CAM1420 4.1 20.6 1.0 C2A A:MNR730 4.3 19.7 1.0 C3A A:MNR730 4.3 19.2 1.0 C2B A:MNR730 4.3 19.8 1.0 C3D A:MNR730 4.3 17.4 1.0 C2C A:MNR730 4.3 19.7 1.0 C3B A:MNR730 4.3 20.3 1.0 C2D A:MNR730 4.3 18.1 1.0 C3C A:MNR730 4.3 19.4 1.0 N A:GLY359 4.4 16.8 1.0 N A:LEU358 4.5 18.9 1.0 C A:CYS357 4.7 18.9 1.0 C4 A:CAM1420 4.7 21.7 1.0 CA A:GLY359 4.9 15.8 1.0 C9 A:CAM1420 5.0 21.0 1.0

Manganese binding site 2 out of 2 in the P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of P450CAM From Pseudomonas Putida Reconstituted with Manganic Protoporphyrin IX within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn731 b:12.8 occ:1.00 MN B:MNR731 0.0 12.8 1.0 NB B:MNR731 2.0 11.6 1.0 ND B:MNR731 2.0 13.2 1.0 NA B:MNR731 2.0 14.2 1.0 NC B:MNR731 2.0 15.7 1.0 SG B:CYS357 2.4 12.4 1.0 C4D B:MNR731 3.1 13.7 1.0 C1C B:MNR731 3.1 15.8 1.0 C1B B:MNR731 3.1 12.0 1.0 C4B B:MNR731 3.1 13.6 1.0 C1D B:MNR731 3.1 15.0 1.0 C1A B:MNR731 3.1 12.8 1.0 C4A B:MNR731 3.1 11.4 1.0 C4C B:MNR731 3.1 15.4 1.0 CB B:CYS357 3.4 12.5 1.0 CHC B:MNR731 3.4 16.0 1.0 CHA B:MNR731 3.4 13.8 1.0 CHB B:MNR731 3.5 13.2 1.0 CHD B:MNR731 3.5 17.4 1.0 CA B:CYS357 4.0 13.3 1.0 C5 B:CAM1421 4.2 17.2 1.0 C2B B:MNR731 4.3 14.0 1.0 C3B B:MNR731 4.3 15.8 1.0 C2D B:MNR731 4.3 14.6 1.0 C3D B:MNR731 4.3 15.6 1.0 C2C B:MNR731 4.3 17.6 1.0 C3C B:MNR731 4.3 18.9 1.0 C2A B:MNR731 4.3 12.7 1.0 C3A B:MNR731 4.4 14.1 1.0 N B:GLY359 4.5 16.6 1.0 N B:LEU358 4.5 15.8 1.0 C B:CYS357 4.7 15.5 1.0 C4 B:CAM1421 4.8 16.1 1.0 C9 B:CAM1421 5.0 13.8 1.0 CA B:GLY359 5.0 16.5 1.0

T.M.Makris, K.Von Koenig, I.Schlichting, S.G.Sligar. The Status of High-Valent Metal Oxo Complexes in the P450 Cytochromes. J.Inorg.Biochem. V. 100 507 2006.
ISSN: ISSN 0162-0134
PubMed: 16510191
DOI: 10.1016/J.JINORGBIO.2006.01.025