Manganese in PDB 1zqz: Dna Polymerase Beta (Pol B) (E.C.2.7.7.7), 31-Kd Domain; Soaked in the Presence of MNCL2 (50 Millimolar)

All present enzymatic activity of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7), 31-Kd Domain; Soaked in the Presence of MNCL2 (50 Millimolar):
2.7.7.7;

The structure of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7), 31-Kd Domain; Soaked in the Presence of MNCL2 (50 Millimolar), PDB code: 1zqz was solved by H.Pelletier, M.R.Sawaya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.70
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	118.565, 62.728, 37.458, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / n/a

The binding sites of Manganese atom in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7), 31-Kd Domain; Soaked in the Presence of MNCL2 (50 Millimolar) (pdb code 1zqz). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7), 31-Kd Domain; Soaked in the Presence of MNCL2 (50 Millimolar), PDB code: 1zqz:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7), 31-Kd Domain; Soaked in the Presence of MNCL2 (50 Millimolar)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7), 31-Kd Domain; Soaked in the Presence of MNCL2 (50 Millimolar) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn343 b:81.4 occ:1.00 OD2 A:ASP145 2.3 37.5 1.0 ND1 A:HIS252 3.0 38.8 1.0 O A:HOH455 3.1 70.6 1.0 CG A:ASP145 3.3 37.4 1.0 CE1 A:HIS252 3.4 43.0 1.0 OD1 A:ASP145 3.7 42.6 1.0 O A:TYR250 3.9 48.6 1.0 CG A:HIS252 4.2 37.1 1.0 NE2 A:GLN240 4.2 55.6 1.0 N A:HIS252 4.3 19.3 1.0 CA A:PRO251 4.3 24.6 1.0 OE1 A:GLU249 4.4 90.5 1.0 C A:PRO251 4.6 28.5 1.0 NE2 A:HIS252 4.6 48.6 1.0 CB A:ASP145 4.7 29.4 1.0 C A:TYR250 4.8 37.5 1.0 CB A:HIS252 4.9 36.5 1.0 CE1 A:TYR142 4.9 25.9 1.0 CD1 A:TYR142 5.0 30.2 1.0

Manganese binding site 2 out of 2 in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7), 31-Kd Domain; Soaked in the Presence of MNCL2 (50 Millimolar)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7), 31-Kd Domain; Soaked in the Presence of MNCL2 (50 Millimolar) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn340 b:90.3 occ:1.00 OD2 A:ASP190 2.6 50.3 1.0 OD1 A:ASP190 2.9 42.7 1.0 CG A:ASP190 3.0 42.2 1.0 CB A:ASP192 3.9 47.3 1.0 OD1 A:ASP192 3.9 70.1 1.0 CG A:ASP192 4.1 62.6 1.0 OD2 A:ASP256 4.2 48.7 1.0 CB A:ASP190 4.3 40.6 1.0 CG A:ASP256 4.6 43.4 1.0 OD2 A:ASP192 4.7 69.8 1.0 CB A:ASP256 4.9 35.6 1.0

H.Pelletier, M.R.Sawaya. Characterization of the Metal Ion Binding Helix-Hairpin-Helix Motifs in Human Dna Polymerase Beta By X-Ray Structural Analysis. Biochemistry V. 35 12778 1996.
ISSN: ISSN 0006-2960
PubMed: 8841120
DOI: 10.1021/BI960790I