Atomistry » Manganese » PDB 1xif-1ytm » 1xis
Atomistry »
  Manganese »
    PDB 1xif-1ytm »
      1xis »

Manganese in PDB 1xis: A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose

Enzymatic activity of A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose

All present enzymatic activity of A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose:
5.3.1.5;

Protein crystallography data

The structure of A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose, PDB code: 1xis was solved by M.Whitlow, A.J.Howard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 10.00 / 1.60
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 94.640, 99.970, 103.970, 90.00, 90.00, 90.00
R / Rfree (%) n/a / n/a

Manganese Binding Sites:

The binding sites of Manganese atom in the A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose (pdb code 1xis). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose, PDB code: 1xis:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 1xis

Go back to Manganese Binding Sites List in 1xis
Manganese binding site 1 out of 2 in the A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn391

b:11.3
occ:1.00
 OE2 A:GLU217 2.1 9.8 1.0
O A:HOH737 2.2 12.4 1.0
OD1 A:ASP257 2.2 10.6 1.0
OD2 A:ASP255 2.3 11.2 1.0
NE2 A:HIS220 2.3 8.8 1.0
OD1 A:ASP255 2.3 9.8 1.0
CG A:ASP255 2.6 10.4 1.0
CD A:GLU217 3.0 10.0 1.0
CD2 A:HIS220 3.0 6.8 1.0
CG A:ASP257 3.2 10.7 1.0
OE1 A:GLU217 3.3 15.1 1.0
CE1 A:HIS220 3.4 9.6 1.0
OD2 A:ASP257 3.4 14.0 1.0
O A:HOH393 3.8 19.2 1.0
OD1 A:ASN247 4.1 13.2 1.0
O A:HOH476 4.1 9.7 1.0
CB A:ASP255 4.2 9.4 1.0
CG A:HIS220 4.2 7.0 1.0
ND1 A:HIS220 4.4 9.5 1.0
CG A:GLU217 4.4 9.8 1.0
CB A:ASP257 4.6 8.1 1.0
O A:HOH394 4.6 22.3 0.9
OD2 A:ASP287 4.7 15.0 1.0
O A:HOH663 4.8 23.2 0.9
CE A:LYS183 4.8 10.9 1.0
NZ A:LYS183 4.8 12.8 1.0
MN A:MN392 4.9 11.1 0.8

Manganese binding site 2 out of 2 in 1xis

Go back to Manganese Binding Sites List in 1xis
Manganese binding site 2 out of 2 in the A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 Angstroms Streptomyces Rubiginosus Structures with Xylitol and D-Xylose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn392

b:11.1
occ:0.80
 OE2 A:GLU181 2.1 16.7 1.0
OE1 A:GLU217 2.1 15.1 1.0
OD2 A:ASP287 2.1 15.0 1.0
OD2 A:ASP245 2.2 15.2 1.0
O A:HOH394 2.4 22.3 0.9
O A:HOH395 2.6 37.3 0.8
CD A:GLU181 3.0 13.8 1.0
OE1 A:GLU181 3.2 18.5 1.0
CG A:ASP287 3.3 12.7 1.0
CD A:GLU217 3.3 10.0 1.0
CG A:ASP245 3.3 12.4 1.0
CB A:ASP287 3.6 9.9 1.0
CB A:ASP245 3.9 8.4 1.0
O A:HOH738 4.0 23.8 0.8
O A:HOH737 4.0 12.4 1.0
O A:HOH776 4.0 31.3 0.6
CG A:GLU217 4.0 9.8 1.0
CB A:GLU217 4.1 7.4 1.0
CE1 A:HIS220 4.2 9.6 1.0
OE2 A:GLU217 4.2 9.8 1.0
CG A:GLU181 4.3 9.0 1.0
OD1 A:ASP245 4.3 11.1 1.0
OD1 A:ASP287 4.3 11.0 1.0
O A:HOH395 4.5 24.9 0.6
NE2 A:HIS220 4.8 8.8 1.0
MN A:MN391 4.9 11.3 1.0
ND1 A:HIS220 4.9 9.5 1.0
OD1 A:ASN215 4.9 14.7 1.0

Reference:

M.Whitlow, A.J.Howard, B.C.Finzel, T.L.Poulos, E.Winborne, G.L.Gilliland. A Metal-Mediated Hydride Shift Mechanism For Xylose Isomerase Based on the 1.6 A Streptomyces Rubiginosus Structures with Xylitol and D-Xylose. Proteins V. 9 153 1991.
ISSN: ISSN 0887-3585
PubMed: 2006134
DOI: 10.1002/PROT.340090302
Page generated: Sat Oct 5 13:06:01 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy