Manganese in PDB 1wlj: Human ISG20

The structure of Human ISG20, PDB code: 1wlj was solved by T.Horio, M.Murai, T.Inoue, T.Hamasaki, T.Tanaka, T.Ohgi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.90
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	63.420, 63.420, 97.910, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The binding sites of Manganese atom in the Human ISG20 (pdb code 1wlj). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Human ISG20, PDB code: 1wlj:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in the Human ISG20


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Human ISG20 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn200 b:17.1 occ:1.00 OD2 A:ASP154 2.1 15.4 1.0 OE2 A:GLU13 2.1 16.3 1.0 OD2 A:ASP11 2.2 15.7 1.0 O2P A:U5P300 2.2 18.9 1.0 O3P A:U5P300 2.4 18.6 1.0 P A:U5P300 2.8 19.1 1.0 CD A:GLU13 3.1 16.9 1.0 CG A:ASP11 3.1 15.4 1.0 CG A:ASP154 3.2 14.8 1.0 OD1 A:ASP11 3.4 14.0 1.0 OE1 A:GLU13 3.4 18.9 1.0 CB A:ASP154 3.6 11.5 1.0 MN A:MN201 3.8 15.3 1.0 O5' A:U5P300 3.9 19.2 1.0 O A:CYS12 4.0 14.4 1.0 O1P A:U5P300 4.0 19.8 1.0 O A:HOH348 4.2 18.9 1.0 C5' A:U5P300 4.2 17.9 1.0 CB A:HIS149 4.2 20.3 1.0 OD1 A:ASP154 4.3 14.5 1.0 OE1 A:GLN143 4.3 23.5 1.0 CA A:SER151 4.4 16.6 1.0 ND1 A:HIS149 4.4 18.1 1.0 CG A:GLU13 4.5 16.4 1.0 CB A:ASP11 4.5 13.5 1.0 O A:SER150 4.6 18.2 1.0 CB A:SER151 4.7 17.4 1.0 N A:SER151 4.7 17.8 1.0 O A:HOH350 4.8 17.1 1.0 C A:CYS12 4.8 16.0 1.0 CG A:HIS149 4.8 19.0 1.0 O A:HOH321 4.8 17.5 1.0 C A:SER150 4.9 19.9 1.0

Manganese binding site 2 out of 3 in the Human ISG20


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Human ISG20 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn201 b:15.3 occ:1.00 O3P A:U5P300 2.0 18.6 1.0 OD1 A:ASP11 2.1 14.0 1.0 O A:HOH350 2.2 17.1 1.0 O A:HOH347 2.2 13.5 1.0 O A:HOH348 2.3 18.9 1.0 O A:HOH349 2.3 17.8 1.0 CG A:ASP11 3.3 15.4 1.0 P A:U5P300 3.4 19.1 1.0 OD2 A:ASP11 3.8 15.7 1.0 MN A:MN200 3.8 17.1 1.0 O1P A:U5P300 3.9 19.8 1.0 O A:HOH331 4.0 22.2 1.0 O A:HOH310 4.0 16.6 1.0 O A:HOH305 4.1 12.9 1.0 O A:CYS12 4.1 14.4 1.0 OD1 A:ASP94 4.1 27.4 1.0 C5' A:U5P300 4.2 17.9 1.0 O A:HOH321 4.2 17.5 1.0 O5' A:U5P300 4.2 19.2 1.0 O A:HIS89 4.2 16.6 1.0 OD2 A:ASP94 4.4 27.8 1.0 CB A:ASP11 4.5 13.5 1.0 O2P A:U5P300 4.5 18.9 1.0 N A:CYS12 4.6 14.3 1.0 CG A:ASP94 4.7 22.6 1.0 CA A:ASP11 4.8 15.3 1.0 O A:HOH379 4.8 39.3 1.0

Manganese binding site 3 out of 3 in the Human ISG20


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Human ISG20 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn202 b:30.5 occ:0.50 NE2 A:HIS93 2.4 28.1 1.0 OD2 A:ASP90 2.4 29.2 1.0 CD2 A:HIS93 3.1 27.3 1.0 CG A:ASP90 3.3 26.9 1.0 OD1 A:ASP90 3.4 27.3 1.0 CE1 A:HIS93 3.6 29.7 1.0 CG A:HIS93 4.3 27.3 1.0 ND1 A:HIS93 4.6 28.9 1.0 CB A:ASP90 4.6 22.3 1.0

T.Horio, M.Murai, T.Inoue, T.Hamasaki, T.Tanaka, T.Ohgi. Crystal Structure of Human ISG20, An Interferon-Induced Antiviral Ribonuclease Febs Lett. V. 577 111 2004.
ISSN: ISSN 0014-5793
PubMed: 15527770
DOI: 10.1016/J.FEBSLET.2004.09.074