Manganese in the structure of Structure of Aminopeptidase P From E. Coli (pdb 1wl9)

The binding sites of Manganese atom in the structure of Structure of Aminopeptidase P From E. Coli (pdb code 1wl9). This binding sites where shown with 5.0 Angstroms radius around Manganese atom. The 1wl9 structure was solved by S.C.GRAHAM, C.S.BOND, H.C.FREEMAN, J.M.GUSS, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 38.4-1.9 Space group P6422 a (A) 177.420 b (A) 177.420 c (A) 96.420 alpha (°) 90.00 beta (°) 90.00 gamma (°) 120.00 Rfactor (%) 15.2 Rfree (%) 17 Manganese Binding Sites: Manganese binding site 1 out of 2 in 1wl9 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Manganese in the PDB 1wl9. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asp271, A: His354, A: Val360, A: His361, A: Thr381, A: Glu383, A: Glu406, A: Mn1002, A: Hoh1004, A: Hoh1070, A: Hoh1349, A: Hoh1559, conact list: Atom Atom Distance (A) Mn CB A:Asp271 4.39 Mn OD2 A:Asp271 2.21 Mn OD1 A:Asp271 3.45 Mn CG A:Asp271 3.11 Mn NE2 A:His354 2.18 Mn ND1 A:His354 4.28 Mn CD2 A:His354 3.12 Mn CE1 A:His354 3.18 Mn CG A:His354 4.28 Mn CG2 A:Val360 4.85 Mn NE2 A:His361 4.77 Mn CD2 A:His361 4.97 Mn CB A:Thr381 4.13 Mn CG2 A:Thr381 3.84 Mn OG1 A:Thr381 3.84 Mn OE1 A:Glu383 3.19 Mn OE2 A:Glu383 2.26 Mn CD A:Glu383 3.02 Mn CG A:Glu383 4.37 Mn OE1 A:Glu406 3.55 Mn OE2 A:Glu406 2.12 Mn CD A:Glu406 3.17 Mn CG A:Glu406 4.50 Mn MN A:Mn1002 3.28 Mn O A:Hoh1004 2.05 Mn O A:Hoh1070 2.84 Mn O A:Hoh1349 4.90 Mn O A:Hoh1559 3.82 interactive model: Manganese binding site 2 out of 2 in 1wl9 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Manganese in the PDB 1wl9. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Tyr229, A: Asp260, A: Asp271, A: Ile272, A: Thr273, A: Glu383, A: Arg404, A: Glu406, A: Mn1001, A: Hoh1004, A: Hoh1070, A: Hoh1533, A: Hoh1559, conact list: Atom Atom Distance (A) Mn CE2 A:Tyr229 4.51 Mn CZ A:Tyr229 4.13 Mn CE1 A:Tyr229 4.83 Mn OH A:Tyr229 3.67 Mn CB A:Asp260 4.07 Mn OD2 A:Asp260 2.29 Mn OD1 A:Asp260 2.18 Mn CG A:Asp260 2.56 Mn CA A:Asp260 4.86 Mn O A:Asp271 4.83 Mn CB A:Asp271 4.44 Mn OD2 A:Asp271 3.37 Mn C A:Asp271 4.59 Mn OD1 A:Asp271 2.07 Mn CG A:Asp271 3.05 Mn CA A:Asp271 4.78 Mn O A:Ile272 4.66 Mn N A:Ile272 4.76 Mn C A:Ile272 4.76 Mn N A:Thr273 4.95 Mn CB A:Thr273 4.90 Mn OG1 A:Thr273 3.52 Mn OE1 A:Glu383 3.99 Mn OE2 A:Glu383 4.80 Mn CD A:Glu383 4.72 Mn NE A:Arg404 4.73 Mn NH2 A:Arg404 4.96 Mn OE1 A:Glu406 2.20 Mn CB A:Glu406 4.97 Mn OE2 A:Glu406 3.26 Mn CD A:Glu406 3.05 Mn CG A:Glu406 4.44 Mn MN A:Mn1001 3.28 Mn O A:Hoh1004 2.13 Mn O A:Hoh1070 4.09 Mn O A:Hoh1533 4.49 Mn O A:Hoh1559 2.38 interactive model: