Manganese in PDB 1vzu: Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase

All present enzymatic activity of Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase:
2.4.1.151;

The structure of Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase, PDB code: 1vzu was solved by Y.Zhang, A.Deshpande, Z.Xie, R.Natesh, K.R.Acharya, K.Brew, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.97
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	44.858, 93.684, 93.714, 90.00, 99.00, 90.00
R / Rfree (%)	15.4 / 20.2

The binding sites of Manganese atom in the Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase (pdb code 1vzu). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase, PDB code: 1vzu:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1369 b:8.5 occ:1.00 O1B A:UDP1371 2.1 8.5 1.0 OD2 A:ASP225 2.1 8.5 1.0 OD1 A:ASP227 2.1 10.4 1.0 O A:HOH2408 2.2 8.1 1.0 O1A A:UDP1371 2.3 7.0 1.0 OD2 A:ASP227 2.4 8.6 1.0 CG A:ASP227 2.6 13.3 1.0 CG A:ASP225 3.2 8.9 1.0 PB A:UDP1371 3.3 9.8 1.0 PA A:UDP1371 3.4 9.3 1.0 O3A A:UDP1371 3.5 9.6 1.0 CB A:ASP225 3.6 7.9 1.0 NZ A:LYS359 3.7 7.7 1.0 O3B A:UDP1371 3.7 11.1 1.0 O3' A:UDP1371 3.8 7.4 1.0 O A:HOH2409 4.1 18.4 1.0 CB A:ASP227 4.1 9.3 1.0 O A:HOH2410 4.2 10.8 1.0 OD1 A:ASP225 4.2 7.8 1.0 O A:HOH2237 4.2 22.3 1.0 C5' A:UDP1371 4.2 6.0 1.0 O5' A:UDP1371 4.3 8.1 1.0 O A:HOH2384 4.4 9.6 1.0 O A:HOH2238 4.5 9.0 1.0 O2A A:UDP1371 4.5 8.9 1.0 O A:HOH2239 4.5 9.3 1.0 O2B A:UDP1371 4.5 7.8 1.0 C3' A:UDP1371 4.6 9.1 1.0 CB A:ALA282 4.7 6.2 1.0 C4' A:UDP1371 4.8 7.1 1.0 O A:ASP227 4.8 9.6 1.0 N A:ASP227 4.8 6.1 1.0 CA A:ASP227 4.9 10.8 1.0 O3 A:LAT1370 4.9 24.3 0.6 CA A:ASP225 5.0 7.2 1.0

Manganese binding site 2 out of 2 in the Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn2369 b:8.9 occ:1.00 O1B B:UDP2371 2.1 7.8 1.0 OD2 B:ASP1225 2.1 7.8 1.0 OD1 B:ASP1227 2.2 6.7 1.0 O1A B:UDP2371 2.2 8.0 1.0 O B:HOH4434 2.2 8.7 1.0 OD2 B:ASP1227 2.4 7.4 1.0 CG B:ASP1227 2.6 9.2 1.0 CG B:ASP1225 3.1 10.9 1.0 PB B:UDP2371 3.2 9.9 1.0 PA B:UDP2371 3.3 9.7 1.0 O3A B:UDP2371 3.5 7.8 1.0 CB B:ASP1225 3.6 5.9 1.0 NZ B:LYS1359 3.6 10.2 1.0 O3B B:UDP2371 3.6 9.9 1.0 O3' B:UDP2371 3.9 6.4 1.0 CB B:ASP1227 4.1 7.8 1.0 OD1 B:ASP1225 4.2 7.7 1.0 O B:HOH4435 4.2 9.6 1.0 O B:HOH4440 4.2 19.2 1.0 O5' B:UDP2371 4.3 8.6 1.0 C5' B:UDP2371 4.3 6.0 1.0 O B:HOH4211 4.3 21.0 1.0 O B:HOH4409 4.4 9.5 1.0 O B:HOH4216 4.4 9.7 1.0 O2A B:UDP2371 4.5 12.3 1.0 O2B B:UDP2371 4.5 8.5 1.0 O B:HOH4217 4.5 8.8 1.0 C3' B:UDP2371 4.6 8.1 1.0 CB B:ALA1282 4.6 5.2 1.0 O B:ASP1227 4.7 10.2 1.0 C4' B:UDP2371 4.8 7.9 1.0 N B:ASP1227 4.9 7.5 1.0 CA B:ASP1227 4.9 6.8 1.0 CA B:ASP1225 5.0 3.2 1.0

Y.Zhang, A.Deshpande, Z.Xie, R.Natesh, K.R.Acharya, K.Brew. Roles of Active Site Tryptophans in Substrate Binding and Catalysis By Alpha-1,3 Galactosyltransferase. Glycobiology V. 14 1295 2004.
ISSN: ISSN 0959-6658
PubMed: 15229192
DOI: 10.1093/GLYCOB/CWH119