Manganese in PDB 1var: Mitochondrial Manganese Superoxide Dismutase Variant with Ile 58 Replaced By Thr

All present enzymatic activity of Mitochondrial Manganese Superoxide Dismutase Variant with Ile 58 Replaced By Thr:
1.15.1.1;

The structure of Mitochondrial Manganese Superoxide Dismutase Variant with Ile 58 Replaced By Thr, PDB code: 1var was solved by G.E.O.Borgstahl, H.E.Parge, J.A.Tainer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.50
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	75.000, 78.100, 68.100, 90.00, 90.00, 90.00
R / Rfree (%)	17 / n/a

The binding sites of Manganese atom in the Mitochondrial Manganese Superoxide Dismutase Variant with Ile 58 Replaced By Thr (pdb code 1var). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Mitochondrial Manganese Superoxide Dismutase Variant with Ile 58 Replaced By Thr, PDB code: 1var:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Mitochondrial Manganese Superoxide Dismutase Variant with Ile 58 Replaced By Thr


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Mitochondrial Manganese Superoxide Dismutase Variant with Ile 58 Replaced By Thr within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn199 b:26.1 occ:1.00 O A:HOH200 2.0 39.1 1.0 OD2 A:ASP159 2.0 9.4 1.0 NE2 A:HIS163 2.0 13.2 1.0 NE2 A:HIS74 2.0 26.9 1.0 NE2 A:HIS26 2.1 40.0 1.0 H2 A:HOH200 2.8 0.0 1.0 CE1 A:HIS74 2.9 30.5 1.0 CE1 A:HIS163 3.0 18.6 1.0 CE1 A:HIS26 3.0 32.3 1.0 CD2 A:HIS163 3.0 9.7 1.0 CD2 A:HIS26 3.0 30.8 1.0 CD2 A:HIS74 3.1 20.5 1.0 CG A:ASP159 3.2 16.3 1.0 HE21 A:GLN143 3.6 0.0 1.0 OD1 A:ASP159 3.8 17.1 1.0 HH A:TYR34 3.9 0.0 1.0 ND1 A:HIS74 4.0 23.1 1.0 ND1 A:HIS163 4.1 16.2 1.0 ND1 A:HIS26 4.1 22.5 1.0 CG A:HIS163 4.2 7.3 1.0 CG A:HIS26 4.2 27.4 1.0 CG A:HIS74 4.2 19.4 1.0 CB A:ASP159 4.4 9.8 1.0 NE2 A:GLN143 4.5 8.0 1.0 CZ2 A:TRP123 4.6 12.1 1.0 CB A:TRP161 4.7 14.9 1.0 CG A:TRP161 4.8 20.5 1.0 OH A:TYR34 4.8 27.3 1.0 HD1 A:HIS74 4.9 0.0 1.0 HD1 A:HIS163 4.9 0.0 1.0 CD1 A:TRP161 5.0 19.7 1.0 CB A:HIS30 5.0 26.0 1.0 HD1 A:HIS26 5.0 0.0 1.0

Manganese binding site 2 out of 2 in the Mitochondrial Manganese Superoxide Dismutase Variant with Ile 58 Replaced By Thr


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Mitochondrial Manganese Superoxide Dismutase Variant with Ile 58 Replaced By Thr within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn199 b:27.2 occ:1.00 OD2 B:ASP159 1.9 9.7 1.0 NE2 B:HIS163 2.0 9.9 1.0 O B:HOH200 2.0 19.8 1.0 NE2 B:HIS74 2.0 15.4 1.0 NE2 B:HIS26 2.0 39.4 1.0 H2 B:HOH200 2.0 0.0 1.0 CE1 B:HIS74 2.9 14.8 1.0 CE1 B:HIS163 2.9 15.3 1.0 CE1 B:HIS26 3.0 32.8 1.0 CD2 B:HIS163 3.0 8.6 1.0 CD2 B:HIS26 3.0 31.5 1.0 CG B:ASP159 3.0 18.1 1.0 CD2 B:HIS74 3.1 13.6 1.0 OD1 B:ASP159 3.5 20.5 1.0 HE21 B:GLN143 3.9 0.0 1.0 ND1 B:HIS74 4.0 9.0 1.0 ND1 B:HIS163 4.0 13.1 1.0 ND1 B:HIS26 4.1 27.0 1.0 CG B:HIS163 4.1 12.5 1.0 CG B:HIS26 4.1 29.4 1.0 HH B:TYR34 4.2 0.0 1.0 CG B:HIS74 4.2 9.0 1.0 CB B:ASP159 4.3 12.1 1.0 CZ2 B:TRP123 4.5 7.5 1.0 NE2 B:GLN143 4.8 15.2 1.0 CB B:TRP161 4.8 17.5 1.0 CB B:ALA164 4.8 18.7 1.0 CG B:TRP161 4.9 18.6 1.0 HD1 B:HIS74 4.9 0.0 1.0 HD1 B:HIS163 4.9 0.0 1.0 HD1 B:HIS26 4.9 0.0 1.0

G.E.Borgstahl, H.E.Parge, M.J.Hickey, M.J.Johnson, M.Boissinot, R.A.Hallewell, J.R.Lepock, D.E.Cabelli, J.A.Tainer. Human Mitochondrial Manganese Superoxide Dismutase Polymorphic Variant ILE58THR Reduces Activity By Destabilizing the Tetrameric Interface. Biochemistry V. 35 4287 1996.
ISSN: ISSN 0006-2960
PubMed: 8605177
DOI: 10.1021/BI951892W