Manganese in PDB 1uzz: Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose

The structure of Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose, PDB code: 1uzz was solved by K.Turton, R.Natesh, N.Thiyagarajan, J.A.Chaddock, K.R.Acharya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.13
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	55.280, 55.370, 86.930, 86.23, 75.37, 82.13
R / Rfree (%)	19.1 / 24.1

The structure of Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose also contains other interesting chemical elements:

Calcium

(Ca)

4 atoms

The binding sites of Manganese atom in the Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose (pdb code 1uzz). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose, PDB code: 1uzz:
Jump to Manganese binding site number: 1; 2; 3; 4;

Manganese binding site 1 out of 4 in the Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1242 b:15.4 occ:1.00 OD2 A:ASP129 2.1 13.5 1.0 OD1 A:ASP136 2.2 12.6 1.0 O A:HOH2249 2.2 24.4 1.0 OE2 A:GLU127 2.2 14.3 1.0 O A:HOH2250 2.3 11.0 1.0 NE2 A:HIS142 2.4 11.3 1.0 CG A:ASP129 3.1 13.4 1.0 CG A:ASP136 3.1 11.1 1.0 CD A:GLU127 3.2 14.7 1.0 CE1 A:HIS142 3.3 13.5 1.0 CD2 A:HIS142 3.4 12.4 1.0 CB A:ASP129 3.5 14.5 1.0 OE1 A:GLU127 3.6 15.4 1.0 OD2 A:ASP136 3.6 13.7 1.0 O A:HOH2140 4.0 21.8 1.0 OG A:SER152 4.1 13.3 1.0 OD1 A:ASP129 4.2 14.7 1.0 CB A:ASP136 4.2 13.6 1.0 CA A:CA1241 4.3 18.8 1.0 ND1 A:HIS142 4.4 12.7 1.0 O A:HOH2162 4.5 14.0 1.0 O A:ILE150 4.5 17.9 1.0 CG A:HIS142 4.5 11.9 1.0 CG A:GLU127 4.6 13.9 1.0 CD A:PRO137 4.6 15.1 1.0 NE1 A:TRP135 4.7 18.4 1.0 CA A:ASP136 4.9 14.6 1.0 CA A:ASP129 4.9 15.6 1.0

Manganese binding site 2 out of 4 in the Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn1242 b:16.7 occ:1.00 OD2 B:ASP129 2.2 12.3 1.0 OD1 B:ASP136 2.2 15.1 1.0 OE2 B:GLU127 2.2 19.1 1.0 O B:HOH2241 2.2 15.9 1.0 O B:HOH2242 2.3 13.7 1.0 NE2 B:HIS142 2.5 16.7 1.0 CG B:ASP129 3.2 14.6 1.0 CG B:ASP136 3.2 14.0 1.0 CD B:GLU127 3.2 17.8 1.0 CE1 B:HIS142 3.3 14.9 1.0 CB B:ASP129 3.5 14.2 1.0 OE1 B:GLU127 3.5 16.8 1.0 CD2 B:HIS142 3.6 15.4 1.0 OD2 B:ASP136 3.7 16.6 1.0 O B:HOH2138 3.9 9.7 1.0 OG B:SER152 4.3 13.8 1.0 CA B:CA1241 4.3 19.4 1.0 CB B:ASP136 4.3 18.1 1.0 OD1 B:ASP129 4.3 16.1 1.0 O B:ILE150 4.5 14.0 1.0 O B:HOH2162 4.5 18.2 1.0 ND1 B:HIS142 4.5 16.0 1.0 CG B:GLU127 4.6 17.4 1.0 CG B:HIS142 4.6 13.5 1.0 CD B:PRO137 4.9 20.6 1.0 NE1 B:TRP135 4.9 20.0 1.0 CA B:ASP136 4.9 18.2 1.0 CA B:ASP129 5.0 15.2 1.0

Manganese binding site 3 out of 4 in the Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn1242 b:22.7 occ:1.00 OD2 C:ASP129 2.1 15.8 1.0 OD1 C:ASP136 2.1 29.9 1.0 O C:HOH2187 2.2 11.6 1.0 O C:HOH2188 2.3 22.3 1.0 OE2 C:GLU127 2.4 20.9 1.0 NE2 C:HIS142 2.5 22.9 1.0 CG C:ASP129 3.2 20.8 1.0 CE1 C:HIS142 3.3 22.3 1.0 CD C:GLU127 3.3 20.6 1.0 CG C:ASP136 3.3 27.2 1.0 OE1 C:GLU127 3.5 22.1 1.0 CB C:ASP129 3.6 19.9 1.0 CD2 C:HIS142 3.6 20.5 1.0 O C:HOH2099 4.0 19.8 1.0 OG C:SER152 4.0 22.0 1.0 OD2 C:ASP136 4.1 31.2 1.0 CA C:CA1241 4.2 25.6 1.0 CB C:ASP136 4.3 26.5 1.0 OD1 C:ASP129 4.3 21.3 1.0 O C:HOH2115 4.3 20.7 1.0 ND1 C:HIS142 4.5 21.4 1.0 O C:ILE150 4.5 24.3 1.0 CG C:HIS142 4.7 21.3 1.0 CG C:GLU127 4.7 20.4 1.0 CD C:PRO137 4.7 25.9 1.0 CA C:ASP136 4.8 25.9 1.0 NE1 C:TRP135 4.9 26.8 1.0

Manganese binding site 4 out of 4 in the Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Erythrina Cristagalli Bound to N-Linked Oligosaccharide and Lactose within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn1243 b:18.5 occ:1.00 OD1 D:ASP136 2.1 16.4 1.0 OD2 D:ASP129 2.2 16.7 1.0 OE2 D:GLU127 2.2 20.6 1.0 O D:HOH2199 2.2 14.0 1.0 O D:HOH2198 2.3 20.3 1.0 NE2 D:HIS142 2.6 18.1 1.0 CG D:ASP129 3.1 13.4 1.0 CG D:ASP136 3.2 18.6 1.0 CD D:GLU127 3.2 20.0 1.0 CE1 D:HIS142 3.4 15.0 1.0 CB D:ASP129 3.5 17.1 1.0 OE1 D:GLU127 3.6 18.7 1.0 CD2 D:HIS142 3.7 17.6 1.0 OD2 D:ASP136 3.8 17.5 1.0 O D:HOH2106 4.0 11.6 1.0 OG D:SER152 4.2 12.3 1.0 CB D:ASP136 4.2 18.5 1.0 CA D:CA1242 4.3 23.9 1.0 OD1 D:ASP129 4.3 16.9 1.0 O D:HOH2125 4.3 25.4 1.0 CG D:GLU127 4.5 16.9 1.0 ND1 D:HIS142 4.6 13.8 1.0 O D:ILE150 4.6 21.7 1.0 CG D:HIS142 4.7 16.2 1.0 NE1 D:TRP135 4.8 18.5 1.0 CA D:ASP136 4.9 19.3 1.0 CD D:PRO137 4.9 19.8 1.0 CA D:ASP129 5.0 18.8 1.0 CD1 D:TRP135 5.0 18.8 1.0

K.Turton, R.Natesh, N.Thiyagarajan, J.A.Chaddock, K.R.Acharya. Crystal Structures of Erythrina Cristagalli Lectin with Bound N-Linked Oligosaccharide and Lactose. Glycobiology V. 14 923 2004.
ISSN: ISSN 0959-6658
PubMed: 15201215
DOI: 10.1093/GLYCOB/CWH114