Manganese in PDB 1u3e: Dna Binding and Cleavage By the Hnh Homing Endonuclease I-Hmui

The structure of Dna Binding and Cleavage By the Hnh Homing Endonuclease I-Hmui, PDB code: 1u3e was solved by B.W.Shen, M.Landthaler, D.A.Shub, B.L.Stoddard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.92
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	45.000, 108.300, 248.100, 90.00, 90.00, 90.00
R / Rfree (%)	21.6 / 24.7

The structure of Dna Binding and Cleavage By the Hnh Homing Endonuclease I-Hmui also contains other interesting chemical elements:

Strontium

(Sr)

4 atoms

The binding sites of Manganese atom in the Dna Binding and Cleavage By the Hnh Homing Endonuclease I-Hmui (pdb code 1u3e). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Dna Binding and Cleavage By the Hnh Homing Endonuclease I-Hmui, PDB code: 1u3e:

Manganese binding site 1 out of 1 in the Dna Binding and Cleavage By the Hnh Homing Endonuclease I-Hmui


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Dna Binding and Cleavage By the Hnh Homing Endonuclease I-Hmui within 5.0Å range: probe atom residue distance (Å) B Occ M:Mn413 b:20.6 occ:1.00 O M:HOH421 1.9 26.8 1.0 O M:HOH420 1.9 44.9 1.0 OP3 C:DG23 2.3 45.0 1.0 OD1 M:ASN96 2.4 37.6 1.0 OD1 M:ASP74 2.5 46.0 1.0 O3' B:DC22 2.7 44.1 1.0 O M:HOH422 3.4 63.0 1.0 CG M:ASN96 3.5 38.5 1.0 CG M:ASP74 3.6 45.1 1.0 C3' B:DC22 3.7 49.6 1.0 P C:DG23 3.7 39.9 1.0 ND2 M:ASN96 3.8 40.3 1.0 O M:HIS75 4.1 41.0 1.0 ND2 M:ASN99 4.2 48.4 1.0 OP1 C:DG23 4.2 36.3 1.0 OD2 M:ASP74 4.3 42.7 1.0 O5' C:DG23 4.3 33.5 1.0 CB M:ASP74 4.4 42.7 1.0 N M:HIS75 4.5 42.0 1.0 O M:ASN96 4.7 35.4 1.0 C2' B:DC22 4.7 51.0 1.0 C4' B:DC22 4.7 51.6 1.0 OP2 C:DG23 4.7 36.1 1.0 NH1 M:ARG103 4.8 76.8 1.0 CB M:ASN96 4.8 36.5 1.0 CA M:ASP74 4.8 40.6 1.0 C M:HIS75 4.9 41.2 1.0

B.W.Shen, M.Landthaler, D.A.Shub, B.L.Stoddard. Dna Binding and Cleavage By the Hnh Homing Endonuclease I-Hmui. J.Mol.Biol. V. 342 43 2004.
ISSN: ISSN 0022-2836
PubMed: 15313606
DOI: 10.1016/J.JMB.2004.07.032