Manganese in PDB 1twa: Rna Polymerase II Complexed with Atp

All present enzymatic activity of Rna Polymerase II Complexed with Atp:
2.7.7.6;

The structure of Rna Polymerase II Complexed with Atp, PDB code: 1twa was solved by K.D.Westover, D.A.Bushnell, R.D.Kornberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 3.20
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	123.000, 223.000, 374.000, 90.00, 90.00, 90.00
R / Rfree (%)	22.7 / 24.6

The structure of Rna Polymerase II Complexed with Atp also contains other interesting chemical elements:

Zinc

(Zn)

8 atoms

The binding sites of Manganese atom in the Rna Polymerase II Complexed with Atp (pdb code 1twa). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Rna Polymerase II Complexed with Atp, PDB code: 1twa:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Rna Polymerase II Complexed with Atp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Rna Polymerase II Complexed with Atp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn3009 b:24.7 occ:1.00 OD1 A:ASP481 2.0 23.3 1.0 O2G A:ATP3011 2.0 71.3 1.0 OD2 A:ASP483 2.0 14.8 1.0 OD2 A:ASP485 2.1 18.9 1.0 O1B A:ATP3011 2.5 74.4 1.0 CG A:ASP481 3.0 20.3 1.0 CG A:ASP483 3.1 15.8 1.0 CG A:ASP485 3.1 16.8 1.0 O2B A:ATP3011 3.2 74.0 1.0 PB A:ATP3011 3.3 74.3 1.0 OD1 A:ASP485 3.3 15.2 1.0 PG A:ATP3011 3.4 73.9 1.0 OD2 A:ASP481 3.4 22.1 1.0 OD1 A:ASP483 3.5 17.9 1.0 O3B A:ATP3011 3.7 74.0 1.0 MN A:MN3010 3.9 29.1 1.0 O1G A:ATP3011 4.2 73.9 1.0 CB A:ASP481 4.3 17.4 1.0 O3G A:ATP3011 4.4 74.3 1.0 CB A:ASP483 4.4 15.7 1.0 CB A:ASP485 4.4 15.2 1.0 N A:ASP481 4.5 17.6 1.0 O A:ASP481 4.6 18.9 1.0 N A:ASP483 4.6 18.2 1.0 N A:ASP485 4.6 16.5 1.0 C A:ASP481 4.7 18.0 1.0 NZ B:LYS987 4.7 45.8 1.0 CA A:ASP481 4.7 16.8 1.0 O3A A:ATP3011 4.8 73.5 1.0 CA A:ASP483 4.9 17.2 1.0 CA A:ASP485 4.9 16.4 1.0 C A:ASP483 4.9 16.5 1.0

Manganese binding site 2 out of 2 in the Rna Polymerase II Complexed with Atp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Rna Polymerase II Complexed with Atp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn3010 b:29.1 occ:1.00 O1B A:ATP3011 1.8 74.4 1.0 OD1 A:ASP483 2.0 17.9 1.0 OD2 A:ASP481 2.1 22.1 1.0 OD2 B:ASP837 2.4 27.8 1.0 O1A A:ATP3011 2.7 69.6 1.0 PB A:ATP3011 2.9 74.3 1.0 O3A A:ATP3011 3.0 73.5 1.0 CG A:ASP483 3.2 15.8 1.0 CG A:ASP481 3.2 20.3 1.0 CG B:ASP837 3.4 25.7 1.0 PA A:ATP3011 3.4 72.4 1.0 OD1 A:ASP481 3.7 23.3 1.0 OD2 A:ASP483 3.8 14.8 1.0 O2B A:ATP3011 3.8 74.0 1.0 NE2 B:GLN986 3.8 55.8 1.0 NH2 B:ARG1020 3.9 41.8 1.0 MN A:MN3009 3.9 24.7 1.0 CB B:ASP837 4.0 25.1 1.0 O3B A:ATP3011 4.1 74.0 1.0 CB A:ASP483 4.3 15.7 1.0 CB A:ASP481 4.4 17.4 1.0 OE1 B:GLN986 4.4 58.8 1.0 OD1 B:ASP837 4.5 24.1 1.0 O2A A:ATP3011 4.5 71.1 1.0 CD B:GLN986 4.5 53.6 1.0 O5' A:ATP3011 4.6 73.3 1.0 O2G A:ATP3011 4.7 71.3 1.0 C8 A:ATP3011 4.9 79.3 1.0

K.D.Westover, D.A.Bushnell, R.D.Kornberg. Structural Basis of Transcription: Nucleotide Selection By Rotation in the Rna Polymerase II Active Center. Cell(Cambridge,Mass.) V. 119 481 2004.
ISSN: ISSN 0092-8674
PubMed: 15537538
DOI: 10.1016/J.CELL.2004.10.016