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Manganese in PDB 1tvy: Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese

Enzymatic activity of Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese

All present enzymatic activity of Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese:
2.4.1.22; 2.4.1.38; 2.4.1.90;

Protein crystallography data

The structure of Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese, PDB code: 1tvy was solved by B.Ramakrishnan, E.Boeggeman, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 26.60 / 2.30
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 50.476, 93.894, 145.054, 90.00, 90.00, 90.00
R / Rfree (%) 19.4 / 24.6

Manganese Binding Sites:

The binding sites of Manganese atom in the Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese (pdb code 1tvy). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese, PDB code: 1tvy:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 1tvy

Go back to Manganese Binding Sites List in 1tvy
Manganese binding site 1 out of 2 in the Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn403

b:15.5
occ:1.00
O1A A:GDU404 1.9 15.3 1.0
NE2 A:HIS344 2.1 19.6 1.0
OD1 A:ASP254 2.2 14.7 1.0
O A:HOH1007 2.3 11.1 1.0
NE2 A:HIS347 2.3 15.5 1.0
O1B A:GDU404 2.3 15.1 1.0
CE1 A:HIS344 3.0 20.6 1.0
CG A:ASP254 3.1 14.8 1.0
CD2 A:HIS344 3.2 20.0 1.0
CD2 A:HIS347 3.2 16.4 1.0
OD2 A:ASP254 3.3 11.7 1.0
CE1 A:HIS347 3.4 16.6 1.0
PA A:GDU404 3.4 16.4 1.0
PB A:GDU404 3.5 16.7 1.0
O3A A:GDU404 3.6 18.9 1.0
O3B A:GDU404 3.9 12.5 1.0
O2A A:GDU404 4.0 21.6 1.0
O3D A:GDU404 4.1 14.1 1.0
O A:HOH1159 4.1 17.7 1.0
ND1 A:HIS344 4.1 19.0 1.0
CG A:HIS344 4.3 20.3 1.0
O A:HOH1177 4.3 27.2 1.0
CB A:ASP254 4.4 12.9 1.0
NH2 A:ARG191 4.4 15.2 1.0
CG A:HIS347 4.4 18.2 1.0
ND1 A:HIS347 4.4 16.2 1.0
NH1 A:ARG191 4.5 13.8 1.0
O5D A:GDU404 4.6 17.1 1.0
C3D A:GDU404 4.6 14.5 1.0
C5D A:GDU404 4.8 14.0 1.0
O2B A:GDU404 4.8 18.1 1.0
OD2 A:ASP252 4.8 17.9 1.0
NZ A:LYS279 4.9 17.2 1.0
CZ A:ARG191 5.0 15.8 1.0

Manganese binding site 2 out of 2 in 1tvy

Go back to Manganese Binding Sites List in 1tvy
Manganese binding site 2 out of 2 in the Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Beta-1,4-Galactosyltransferase Mutant MET344HIS (M344H-Gal- T1) Complex with Udp-Galactose and Manganese within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn805

b:13.6
occ:1.00
O1A B:GDU806 2.0 17.1 1.0
NE2 B:HIS344 2.2 13.1 1.0
NE2 B:HIS347 2.2 18.4 1.0
OD1 B:ASP254 2.3 17.9 1.0
O1B B:GDU806 2.3 11.7 1.0
O B:HOH1004 2.4 16.3 1.0
CE1 B:HIS344 3.1 12.0 1.0
CG B:ASP254 3.1 17.2 1.0
CD2 B:HIS347 3.2 18.4 1.0
CE1 B:HIS347 3.2 18.1 1.0
CD2 B:HIS344 3.3 13.5 1.0
PA B:GDU806 3.3 18.4 1.0
OD2 B:ASP254 3.4 12.5 1.0
PB B:GDU806 3.4 17.6 1.0
O3A B:GDU806 3.6 17.7 1.0
O3B B:GDU806 3.9 17.7 1.0
O2A B:GDU806 4.0 22.6 1.0
O3D B:GDU806 4.0 15.4 1.0
ND1 B:HIS344 4.3 13.2 1.0
ND1 B:HIS347 4.3 19.1 1.0
CG B:HIS347 4.3 19.6 1.0
O B:HOH1249 4.3 33.9 1.0
CG B:HIS344 4.4 14.7 1.0
O B:HOH1179 4.4 20.3 1.0
CB B:ASP254 4.4 13.3 1.0
NH2 B:ARG191 4.5 9.0 1.0
NH1 B:ARG191 4.5 11.8 1.0
O5D B:GDU806 4.6 19.5 1.0
C3D B:GDU806 4.7 14.1 1.0
OD2 B:ASP252 4.7 16.5 1.0
NZ B:LYS279 4.7 23.6 1.0
O2B B:GDU806 4.7 18.2 1.0
C5D B:GDU806 4.9 14.8 1.0

Reference:

B.Ramakrishnan, E.Boeggeman, P.K.Qasba. Effect of the MET344HIS Mutation on the Conformational Dynamics of Bovine Beta-1,4-Galactosyltransferase: Crystal Structure of the MET344HIS Mutant in Complex with Chitobiose Biochemistry V. 43 12513 2004.
ISSN: ISSN 0006-2960
PubMed: 15449940
DOI: 10.1021/BI049007+
Page generated: Sat Oct 5 12:35:14 2024

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