Manganese in PDB 1t4r: Arginase-Descarboxy-Nor-Noha Complex
Enzymatic activity of Arginase-Descarboxy-Nor-Noha Complex
All present enzymatic activity of Arginase-Descarboxy-Nor-Noha Complex:
3.5.3.1;
Protein crystallography data
The structure of Arginase-Descarboxy-Nor-Noha Complex, PDB code: 1t4r
was solved by
E.Cama,
S.Pethe,
J.-L.Boucher,
H.Shoufa,
F.A.Emig,
D.E.Ash,
R.E.Viola,
D.Mansuy,
D.W.Christianson,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
28.90 /
2.60
|
Space group
|
P 32
|
Cell size a, b, c (Å), α, β, γ (°)
|
88.300,
88.300,
113.200,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
25.5 /
29.1
|
Manganese Binding Sites:
The binding sites of Manganese atom in the Arginase-Descarboxy-Nor-Noha Complex
(pdb code 1t4r). This binding sites where shown within
5.0 Angstroms radius around Manganese atom.
In total 6 binding sites of Manganese where determined in the
Arginase-Descarboxy-Nor-Noha Complex, PDB code: 1t4r:
Jump to Manganese binding site number:
1;
2;
3;
4;
5;
6;
Manganese binding site 1 out
of 6 in 1t4r
Go back to
Manganese Binding Sites List in 1t4r
Manganese binding site 1 out
of 6 in the Arginase-Descarboxy-Nor-Noha Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 1 of Arginase-Descarboxy-Nor-Noha Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn500
b:30.4
occ:1.00
|
ND1
|
A:HIS126
|
2.0
|
45.8
|
1.0
|
OD2
|
A:ASP234
|
2.2
|
42.1
|
1.0
|
OD1
|
A:ASP124
|
2.3
|
40.2
|
1.0
|
OD2
|
A:ASP232
|
2.3
|
39.3
|
1.0
|
OD1
|
A:ASP234
|
2.4
|
40.2
|
1.0
|
O16
|
A:AHI1000
|
2.5
|
62.4
|
1.0
|
CG
|
A:ASP234
|
2.7
|
41.8
|
1.0
|
CE1
|
A:HIS126
|
2.8
|
44.2
|
1.0
|
MN
|
A:MN501
|
3.0
|
44.6
|
1.0
|
CG
|
A:HIS126
|
3.1
|
44.8
|
1.0
|
CG
|
A:ASP124
|
3.1
|
38.7
|
1.0
|
CG
|
A:ASP232
|
3.1
|
37.5
|
1.0
|
OD2
|
A:ASP124
|
3.3
|
38.5
|
1.0
|
NP1
|
A:AHI1000
|
3.4
|
64.6
|
1.0
|
N10
|
A:AHI1000
|
3.4
|
65.3
|
1.0
|
CB
|
A:HIS126
|
3.7
|
43.9
|
1.0
|
C09
|
A:AHI1000
|
3.7
|
65.8
|
1.0
|
OD1
|
A:ASP232
|
3.7
|
36.6
|
1.0
|
N
|
A:HIS126
|
3.8
|
42.5
|
1.0
|
NE2
|
A:HIS126
|
4.0
|
44.0
|
1.0
|
OG1
|
A:THR246
|
4.0
|
45.0
|
1.0
|
CB
|
A:ASP232
|
4.1
|
37.4
|
1.0
|
CD2
|
A:HIS126
|
4.1
|
43.5
|
1.0
|
CB
|
A:ASP234
|
4.2
|
39.0
|
1.0
|
N
|
A:ALA125
|
4.2
|
40.8
|
1.0
|
CA
|
A:HIS126
|
4.4
|
44.2
|
1.0
|
OD1
|
A:ASP128
|
4.5
|
48.5
|
1.0
|
CB
|
A:ASP124
|
4.5
|
40.8
|
1.0
|
O
|
A:HOH766
|
4.6
|
88.8
|
1.0
|
O
|
A:HOH691
|
4.7
|
29.9
|
1.0
|
C
|
A:ALA125
|
4.7
|
42.6
|
1.0
|
OD2
|
A:ASP128
|
4.8
|
50.2
|
1.0
|
CB
|
A:ALA125
|
4.8
|
40.0
|
1.0
|
CA
|
A:ALA125
|
4.9
|
40.9
|
1.0
|
CA
|
A:ASP124
|
4.9
|
41.1
|
1.0
|
N08
|
A:AHI1000
|
4.9
|
65.6
|
1.0
|
O
|
A:HIS126
|
4.9
|
46.2
|
1.0
|
|
Manganese binding site 2 out
of 6 in 1t4r
Go back to
Manganese Binding Sites List in 1t4r
Manganese binding site 2 out
of 6 in the Arginase-Descarboxy-Nor-Noha Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 2 of Arginase-Descarboxy-Nor-Noha Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn501
b:44.6
occ:1.00
|
OD2
|
A:ASP124
|
2.1
|
38.5
|
1.0
|
OD2
|
A:ASP232
|
2.2
|
39.3
|
1.0
|
OD2
|
A:ASP128
|
2.3
|
50.2
|
1.0
|
O16
|
A:AHI1000
|
2.3
|
62.4
|
1.0
|
ND1
|
A:HIS101
|
2.4
|
49.8
|
1.0
|
O
|
A:HOH766
|
2.8
|
88.8
|
1.0
|
MN
|
A:MN500
|
3.0
|
30.4
|
1.0
|
CG
|
A:ASP124
|
3.1
|
38.7
|
1.0
|
CG
|
A:ASP128
|
3.1
|
49.6
|
1.0
|
CG
|
A:ASP232
|
3.2
|
37.5
|
1.0
|
OD1
|
A:ASP128
|
3.3
|
48.5
|
1.0
|
CE1
|
A:HIS101
|
3.3
|
48.7
|
1.0
|
CG
|
A:HIS101
|
3.4
|
50.9
|
1.0
|
NP1
|
A:AHI1000
|
3.5
|
64.6
|
1.0
|
OD1
|
A:ASP124
|
3.5
|
40.2
|
1.0
|
CB
|
A:ASP232
|
3.6
|
37.4
|
1.0
|
CB
|
A:HIS101
|
3.6
|
50.9
|
1.0
|
OD1
|
A:ASP232
|
4.3
|
36.6
|
1.0
|
NE1
|
A:TRP122
|
4.4
|
40.8
|
1.0
|
CB
|
A:ASP124
|
4.4
|
40.8
|
1.0
|
NE2
|
A:HIS101
|
4.5
|
50.9
|
1.0
|
CD2
|
A:HIS101
|
4.5
|
50.6
|
1.0
|
C09
|
A:AHI1000
|
4.5
|
65.8
|
1.0
|
OD2
|
A:ASP234
|
4.6
|
42.1
|
1.0
|
CB
|
A:ASP128
|
4.6
|
49.5
|
1.0
|
ND1
|
A:HIS126
|
4.6
|
45.8
|
1.0
|
O
|
A:HIS141
|
4.7
|
51.4
|
1.0
|
CZ2
|
A:TRP122
|
4.7
|
41.2
|
1.0
|
N10
|
A:AHI1000
|
4.7
|
65.3
|
1.0
|
CB
|
A:HIS126
|
4.8
|
43.9
|
1.0
|
CG
|
A:GLU277
|
4.8
|
47.5
|
1.0
|
OD1
|
A:ASP234
|
4.8
|
40.2
|
1.0
|
O
|
A:HIS126
|
4.8
|
46.2
|
1.0
|
OE2
|
A:GLU277
|
4.9
|
45.3
|
1.0
|
CE2
|
A:TRP122
|
4.9
|
41.4
|
1.0
|
CA
|
A:ASP232
|
4.9
|
39.9
|
1.0
|
|
Manganese binding site 3 out
of 6 in 1t4r
Go back to
Manganese Binding Sites List in 1t4r
Manganese binding site 3 out
of 6 in the Arginase-Descarboxy-Nor-Noha Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 3 of Arginase-Descarboxy-Nor-Noha Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mn502
b:33.1
occ:1.00
|
ND1
|
B:HIS126
|
2.0
|
37.9
|
1.0
|
OD1
|
B:ASP124
|
2.1
|
30.7
|
1.0
|
OD2
|
B:ASP232
|
2.2
|
38.2
|
1.0
|
OD2
|
B:ASP234
|
2.2
|
36.5
|
1.0
|
OD1
|
B:ASP234
|
2.4
|
38.2
|
1.0
|
O16
|
B:AHI1001
|
2.5
|
62.6
|
1.0
|
CG
|
B:ASP234
|
2.6
|
37.6
|
1.0
|
CE1
|
B:HIS126
|
2.9
|
39.2
|
1.0
|
MN
|
B:MN503
|
3.0
|
35.3
|
1.0
|
CG
|
B:ASP124
|
3.0
|
29.3
|
1.0
|
CG
|
B:ASP232
|
3.0
|
35.3
|
1.0
|
CG
|
B:HIS126
|
3.1
|
36.8
|
1.0
|
OD2
|
B:ASP124
|
3.2
|
27.1
|
1.0
|
NP1
|
B:AHI1001
|
3.4
|
64.6
|
1.0
|
N10
|
B:AHI1001
|
3.6
|
65.1
|
1.0
|
CB
|
B:HIS126
|
3.6
|
35.4
|
1.0
|
OD1
|
B:ASP232
|
3.7
|
36.4
|
1.0
|
C09
|
B:AHI1001
|
3.8
|
65.5
|
1.0
|
N
|
B:HIS126
|
3.9
|
35.5
|
1.0
|
CB
|
B:ASP232
|
3.9
|
34.4
|
1.0
|
NE2
|
B:HIS126
|
4.0
|
38.6
|
1.0
|
N
|
B:ALA125
|
4.1
|
30.7
|
1.0
|
CB
|
B:ASP234
|
4.1
|
35.3
|
1.0
|
CD2
|
B:HIS126
|
4.2
|
36.6
|
1.0
|
OG1
|
B:THR246
|
4.2
|
44.9
|
1.0
|
CA
|
B:HIS126
|
4.4
|
36.0
|
1.0
|
CB
|
B:ASP124
|
4.4
|
29.3
|
1.0
|
OD1
|
B:ASP128
|
4.5
|
41.4
|
1.0
|
O
|
B:HOH716
|
4.6
|
87.4
|
1.0
|
O
|
B:HOH698
|
4.7
|
17.4
|
1.0
|
OD2
|
B:ASP128
|
4.7
|
45.7
|
1.0
|
CB
|
B:ALA125
|
4.8
|
32.2
|
1.0
|
C
|
B:ALA125
|
4.8
|
33.6
|
1.0
|
CA
|
B:ASP124
|
4.8
|
29.8
|
1.0
|
CA
|
B:ALA125
|
4.9
|
33.2
|
1.0
|
O
|
B:HIS126
|
4.9
|
35.3
|
1.0
|
C
|
B:ASP124
|
5.0
|
30.6
|
1.0
|
O
|
B:ASP234
|
5.0
|
34.5
|
1.0
|
|
Manganese binding site 4 out
of 6 in 1t4r
Go back to
Manganese Binding Sites List in 1t4r
Manganese binding site 4 out
of 6 in the Arginase-Descarboxy-Nor-Noha Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 4 of Arginase-Descarboxy-Nor-Noha Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mn503
b:35.3
occ:1.00
|
OD2
|
B:ASP124
|
2.1
|
27.1
|
1.0
|
O16
|
B:AHI1001
|
2.2
|
62.6
|
1.0
|
OD2
|
B:ASP128
|
2.2
|
45.7
|
1.0
|
OD2
|
B:ASP232
|
2.3
|
38.2
|
1.0
|
ND1
|
B:HIS101
|
2.5
|
42.5
|
1.0
|
O
|
B:HOH716
|
2.8
|
87.4
|
1.0
|
MN
|
B:MN502
|
3.0
|
33.1
|
1.0
|
CG
|
B:ASP128
|
3.1
|
42.7
|
1.0
|
CG
|
B:ASP124
|
3.1
|
29.3
|
1.0
|
OD1
|
B:ASP128
|
3.3
|
41.4
|
1.0
|
CG
|
B:ASP232
|
3.3
|
35.3
|
1.0
|
NP1
|
B:AHI1001
|
3.4
|
64.6
|
1.0
|
OD1
|
B:ASP124
|
3.4
|
30.7
|
1.0
|
CG
|
B:HIS101
|
3.4
|
43.4
|
1.0
|
CE1
|
B:HIS101
|
3.4
|
42.0
|
1.0
|
CB
|
B:HIS101
|
3.6
|
44.7
|
1.0
|
CB
|
B:ASP232
|
3.7
|
34.4
|
1.0
|
OD1
|
B:ASP232
|
4.4
|
36.4
|
1.0
|
C09
|
B:AHI1001
|
4.5
|
65.5
|
1.0
|
CB
|
B:ASP124
|
4.5
|
29.3
|
1.0
|
O
|
B:HIS141
|
4.5
|
45.6
|
1.0
|
NE1
|
B:TRP122
|
4.5
|
32.4
|
1.0
|
ND1
|
B:HIS126
|
4.5
|
37.9
|
1.0
|
CB
|
B:ASP128
|
4.5
|
42.9
|
1.0
|
NE2
|
B:HIS101
|
4.5
|
42.8
|
1.0
|
OD2
|
B:ASP234
|
4.6
|
36.5
|
1.0
|
CD2
|
B:HIS101
|
4.6
|
42.0
|
1.0
|
CB
|
B:HIS126
|
4.6
|
35.4
|
1.0
|
N10
|
B:AHI1001
|
4.7
|
65.1
|
1.0
|
CG
|
B:GLU277
|
4.7
|
37.0
|
1.0
|
CZ2
|
B:TRP122
|
4.7
|
31.0
|
1.0
|
OE2
|
B:GLU277
|
4.8
|
37.3
|
1.0
|
OD1
|
B:ASP234
|
4.8
|
38.2
|
1.0
|
O
|
B:HIS126
|
4.8
|
35.3
|
1.0
|
CE2
|
B:TRP122
|
5.0
|
30.6
|
1.0
|
CG
|
B:HIS126
|
5.0
|
36.8
|
1.0
|
|
Manganese binding site 5 out
of 6 in 1t4r
Go back to
Manganese Binding Sites List in 1t4r
Manganese binding site 5 out
of 6 in the Arginase-Descarboxy-Nor-Noha Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 5 of Arginase-Descarboxy-Nor-Noha Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mn504
b:26.6
occ:1.00
|
ND1
|
C:HIS126
|
2.1
|
30.9
|
1.0
|
OD1
|
C:ASP124
|
2.1
|
35.0
|
1.0
|
OD2
|
C:ASP232
|
2.1
|
32.0
|
1.0
|
OD2
|
C:ASP234
|
2.3
|
35.0
|
1.0
|
OD1
|
C:ASP234
|
2.4
|
34.0
|
1.0
|
O16
|
C:AHI1002
|
2.5
|
62.8
|
1.0
|
CG
|
C:ASP234
|
2.6
|
35.2
|
1.0
|
CE1
|
C:HIS126
|
2.9
|
30.4
|
1.0
|
CG
|
C:ASP124
|
3.0
|
34.1
|
1.0
|
MN
|
C:MN505
|
3.0
|
38.8
|
1.0
|
CG
|
C:ASP232
|
3.0
|
30.2
|
1.0
|
CG
|
C:HIS126
|
3.2
|
29.4
|
1.0
|
OD2
|
C:ASP124
|
3.2
|
33.7
|
1.0
|
NP1
|
C:AHI1002
|
3.4
|
64.7
|
1.0
|
N10
|
C:AHI1002
|
3.6
|
65.5
|
1.0
|
CB
|
C:HIS126
|
3.6
|
28.5
|
1.0
|
OD1
|
C:ASP232
|
3.7
|
27.5
|
1.0
|
C09
|
C:AHI1002
|
3.8
|
65.4
|
1.0
|
N
|
C:HIS126
|
3.9
|
32.4
|
1.0
|
CB
|
C:ASP232
|
4.0
|
29.3
|
1.0
|
NE2
|
C:HIS126
|
4.1
|
31.4
|
1.0
|
OG1
|
C:THR246
|
4.1
|
37.8
|
1.0
|
N
|
C:ALA125
|
4.1
|
32.2
|
1.0
|
CB
|
C:ASP234
|
4.2
|
33.3
|
1.0
|
CD2
|
C:HIS126
|
4.2
|
29.4
|
1.0
|
CA
|
C:HIS126
|
4.4
|
32.0
|
1.0
|
CB
|
C:ASP124
|
4.4
|
32.1
|
1.0
|
OD1
|
C:ASP128
|
4.5
|
38.0
|
1.0
|
O
|
C:HOH768
|
4.6
|
87.6
|
1.0
|
OD2
|
C:ASP128
|
4.7
|
38.8
|
1.0
|
C
|
C:ALA125
|
4.8
|
32.9
|
1.0
|
CA
|
C:ASP124
|
4.8
|
31.7
|
1.0
|
CB
|
C:ALA125
|
4.8
|
33.5
|
1.0
|
CA
|
C:ALA125
|
4.8
|
33.4
|
1.0
|
O
|
C:HIS126
|
4.9
|
33.6
|
1.0
|
C
|
C:ASP124
|
4.9
|
32.8
|
1.0
|
|
Manganese binding site 6 out
of 6 in 1t4r
Go back to
Manganese Binding Sites List in 1t4r
Manganese binding site 6 out
of 6 in the Arginase-Descarboxy-Nor-Noha Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 6 of Arginase-Descarboxy-Nor-Noha Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mn505
b:38.8
occ:1.00
|
OD2
|
C:ASP124
|
2.2
|
33.7
|
1.0
|
OD2
|
C:ASP128
|
2.2
|
38.8
|
1.0
|
O16
|
C:AHI1002
|
2.3
|
62.8
|
1.0
|
OD2
|
C:ASP232
|
2.3
|
32.0
|
1.0
|
ND1
|
C:HIS101
|
2.4
|
31.5
|
1.0
|
O
|
C:HOH768
|
2.7
|
87.6
|
1.0
|
MN
|
C:MN504
|
3.0
|
26.6
|
1.0
|
CG
|
C:ASP128
|
3.1
|
36.7
|
1.0
|
CG
|
C:ASP124
|
3.1
|
34.1
|
1.0
|
CG
|
C:ASP232
|
3.3
|
30.2
|
1.0
|
CG
|
C:HIS101
|
3.3
|
33.1
|
1.0
|
CE1
|
C:HIS101
|
3.4
|
32.8
|
1.0
|
OD1
|
C:ASP128
|
3.4
|
38.0
|
1.0
|
NP1
|
C:AHI1002
|
3.4
|
64.7
|
1.0
|
OD1
|
C:ASP124
|
3.5
|
35.0
|
1.0
|
CB
|
C:HIS101
|
3.5
|
35.4
|
1.0
|
CB
|
C:ASP232
|
3.7
|
29.3
|
1.0
|
OD1
|
C:ASP232
|
4.4
|
27.5
|
1.0
|
NE2
|
C:HIS101
|
4.5
|
33.4
|
1.0
|
NE1
|
C:TRP122
|
4.5
|
34.1
|
1.0
|
CD2
|
C:HIS101
|
4.5
|
32.5
|
1.0
|
CB
|
C:ASP124
|
4.5
|
32.1
|
1.0
|
O
|
C:HIS141
|
4.5
|
39.7
|
1.0
|
C09
|
C:AHI1002
|
4.5
|
65.4
|
1.0
|
CB
|
C:ASP128
|
4.5
|
40.0
|
1.0
|
ND1
|
C:HIS126
|
4.6
|
30.9
|
1.0
|
CG
|
C:GLU277
|
4.7
|
37.4
|
1.0
|
OD2
|
C:ASP234
|
4.7
|
35.0
|
1.0
|
CB
|
C:HIS126
|
4.7
|
28.5
|
1.0
|
N10
|
C:AHI1002
|
4.8
|
65.5
|
1.0
|
CZ2
|
C:TRP122
|
4.8
|
34.8
|
1.0
|
OE2
|
C:GLU277
|
4.8
|
35.4
|
1.0
|
O
|
C:HIS126
|
4.8
|
33.6
|
1.0
|
OD1
|
C:ASP234
|
4.9
|
34.0
|
1.0
|
CE2
|
C:TRP122
|
5.0
|
33.6
|
1.0
|
|
Reference:
E.Cama,
S.Pethe,
J.-L.Boucher,
S.Han,
F.A.Emig,
D.E.Ash,
R.E.Viola,
D.Mansuy,
D.W.Christianson.
Inhibitor Coordination Interactions in the Binuclear Manganese Cluster of Arginase Biochemistry V. 43 8987 2004.
ISSN: ISSN 0006-2960
PubMed: 15248756
DOI: 10.1021/BI0491705
Page generated: Sat Oct 5 12:28:15 2024
|