Manganese in PDB 1t4p: Arginase-Dehydro-Abh Complex
Enzymatic activity of Arginase-Dehydro-Abh Complex
All present enzymatic activity of Arginase-Dehydro-Abh Complex:
3.5.3.1;
Protein crystallography data
The structure of Arginase-Dehydro-Abh Complex, PDB code: 1t4p
was solved by
E.Cama,
S.Pethe,
J.-L.Boucher,
S.Han,
F.A.Emig,
D.E.Ash,
R.E.Viola,
D.Mansuy,
D.W.Christianson,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
30.00 /
2.60
|
Space group
|
P 32
|
Cell size a, b, c (Å), α, β, γ (°)
|
88.400,
88.400,
110.520,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
26.4 /
29.2
|
Manganese Binding Sites:
The binding sites of Manganese atom in the Arginase-Dehydro-Abh Complex
(pdb code 1t4p). This binding sites where shown within
5.0 Angstroms radius around Manganese atom.
In total 6 binding sites of Manganese where determined in the
Arginase-Dehydro-Abh Complex, PDB code: 1t4p:
Jump to Manganese binding site number:
1;
2;
3;
4;
5;
6;
Manganese binding site 1 out
of 6 in 1t4p
Go back to
Manganese Binding Sites List in 1t4p
Manganese binding site 1 out
of 6 in the Arginase-Dehydro-Abh Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 1 of Arginase-Dehydro-Abh Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn502
b:37.0
occ:1.00
|
OD2
|
A:ASP232
|
2.1
|
40.5
|
1.0
|
O12
|
A:2BH1000
|
2.2
|
27.5
|
1.0
|
OD2
|
A:ASP128
|
2.3
|
34.8
|
1.0
|
ND1
|
A:HIS101
|
2.3
|
42.0
|
1.0
|
OD2
|
A:ASP124
|
2.4
|
28.5
|
1.0
|
O11
|
A:2BH1000
|
2.5
|
28.1
|
1.0
|
B
|
A:2BH1000
|
2.9
|
29.6
|
1.0
|
CG
|
A:HIS101
|
3.2
|
42.7
|
1.0
|
CG
|
A:ASP232
|
3.2
|
40.9
|
1.0
|
CG
|
A:ASP128
|
3.3
|
35.5
|
1.0
|
MN
|
A:MN504
|
3.3
|
35.2
|
1.0
|
CE1
|
A:HIS101
|
3.3
|
39.4
|
1.0
|
CB
|
A:HIS101
|
3.4
|
44.5
|
1.0
|
CG
|
A:ASP124
|
3.5
|
32.6
|
1.0
|
OD1
|
A:ASP128
|
3.6
|
34.7
|
1.0
|
CB
|
A:ASP232
|
3.6
|
40.1
|
1.0
|
O13
|
A:2BH1000
|
3.7
|
32.8
|
1.0
|
OD1
|
A:ASP124
|
3.8
|
36.1
|
1.0
|
C09
|
A:2BH1000
|
3.9
|
28.4
|
1.0
|
OD1
|
A:ASP232
|
4.3
|
44.9
|
1.0
|
O
|
A:HIS141
|
4.4
|
40.9
|
1.0
|
CD2
|
A:HIS101
|
4.4
|
41.8
|
1.0
|
NE2
|
A:HIS101
|
4.4
|
40.4
|
1.0
|
OE2
|
A:GLU277
|
4.4
|
50.2
|
1.0
|
NE1
|
A:TRP122
|
4.5
|
37.8
|
1.0
|
CG
|
A:GLU277
|
4.5
|
44.8
|
1.0
|
OD2
|
A:ASP234
|
4.6
|
39.6
|
1.0
|
CB
|
A:ASP128
|
4.6
|
36.9
|
1.0
|
CZ2
|
A:TRP122
|
4.6
|
34.3
|
1.0
|
CB
|
A:ASP124
|
4.8
|
33.5
|
1.0
|
CA
|
A:HIS101
|
4.9
|
47.7
|
1.0
|
CE2
|
A:TRP122
|
4.9
|
35.4
|
1.0
|
C08
|
A:2BH1000
|
4.9
|
28.8
|
1.0
|
CA
|
A:ASP232
|
4.9
|
31.2
|
1.0
|
CD
|
A:GLU277
|
5.0
|
47.8
|
1.0
|
ND1
|
A:HIS126
|
5.0
|
38.1
|
1.0
|
|
Manganese binding site 2 out
of 6 in 1t4p
Go back to
Manganese Binding Sites List in 1t4p
Manganese binding site 2 out
of 6 in the Arginase-Dehydro-Abh Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 2 of Arginase-Dehydro-Abh Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn504
b:35.2
occ:1.00
|
O12
|
A:2BH1000
|
2.0
|
27.5
|
1.0
|
OD2
|
A:ASP234
|
2.1
|
39.6
|
1.0
|
ND1
|
A:HIS126
|
2.2
|
38.1
|
1.0
|
OD2
|
A:ASP232
|
2.4
|
40.5
|
1.0
|
OD1
|
A:ASP234
|
2.4
|
40.5
|
1.0
|
OD1
|
A:ASP124
|
2.4
|
36.1
|
1.0
|
CG
|
A:ASP234
|
2.5
|
38.8
|
1.0
|
CE1
|
A:HIS126
|
3.0
|
38.3
|
1.0
|
CG
|
A:ASP232
|
3.1
|
40.9
|
1.0
|
B
|
A:2BH1000
|
3.3
|
29.6
|
1.0
|
O13
|
A:2BH1000
|
3.3
|
32.8
|
1.0
|
CG
|
A:ASP124
|
3.3
|
32.6
|
1.0
|
MN
|
A:MN502
|
3.3
|
37.0
|
1.0
|
CG
|
A:HIS126
|
3.3
|
38.9
|
1.0
|
OD2
|
A:ASP124
|
3.4
|
28.5
|
1.0
|
OD1
|
A:ASP232
|
3.5
|
44.9
|
1.0
|
CB
|
A:HIS126
|
3.8
|
37.7
|
1.0
|
CB
|
A:ASP234
|
4.0
|
36.5
|
1.0
|
N
|
A:HIS126
|
4.1
|
36.2
|
1.0
|
CB
|
A:ASP232
|
4.1
|
40.1
|
1.0
|
O11
|
A:2BH1000
|
4.1
|
28.1
|
1.0
|
N
|
A:ALA125
|
4.2
|
36.4
|
1.0
|
C09
|
A:2BH1000
|
4.2
|
28.4
|
1.0
|
NE2
|
A:HIS126
|
4.2
|
40.0
|
1.0
|
C08
|
A:2BH1000
|
4.3
|
28.8
|
1.0
|
CD2
|
A:HIS126
|
4.4
|
38.5
|
1.0
|
O
|
A:HOH708
|
4.4
|
31.7
|
1.0
|
OD1
|
A:ASP128
|
4.5
|
34.7
|
1.0
|
CA
|
A:HIS126
|
4.5
|
37.3
|
1.0
|
CB
|
A:ASP124
|
4.6
|
33.5
|
1.0
|
CB
|
A:ALA125
|
4.6
|
34.9
|
1.0
|
OD2
|
A:ASP128
|
4.7
|
34.8
|
1.0
|
C
|
A:ALA125
|
4.9
|
35.1
|
1.0
|
CA
|
A:ALA125
|
4.9
|
35.8
|
1.0
|
|
Manganese binding site 3 out
of 6 in 1t4p
Go back to
Manganese Binding Sites List in 1t4p
Manganese binding site 3 out
of 6 in the Arginase-Dehydro-Abh Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 3 of Arginase-Dehydro-Abh Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mn500
b:27.8
occ:1.00
|
O12
|
B:2BH1001
|
2.0
|
27.5
|
1.0
|
ND1
|
B:HIS126
|
2.1
|
31.1
|
1.0
|
OD2
|
B:ASP234
|
2.2
|
37.5
|
1.0
|
OD2
|
B:ASP232
|
2.3
|
40.9
|
1.0
|
OD1
|
B:ASP124
|
2.4
|
38.6
|
1.0
|
OD1
|
B:ASP234
|
2.5
|
36.6
|
1.0
|
CG
|
B:ASP234
|
2.7
|
34.2
|
1.0
|
CE1
|
B:HIS126
|
3.0
|
32.6
|
1.0
|
CG
|
B:ASP232
|
3.2
|
40.3
|
1.0
|
CG
|
B:HIS126
|
3.2
|
30.5
|
1.0
|
B
|
B:2BH1001
|
3.2
|
29.6
|
1.0
|
CG
|
B:ASP124
|
3.2
|
35.6
|
1.0
|
MN
|
B:MN501
|
3.3
|
30.5
|
1.0
|
O13
|
B:2BH1001
|
3.3
|
32.8
|
1.0
|
OD2
|
B:ASP124
|
3.4
|
32.0
|
1.0
|
CB
|
B:HIS126
|
3.6
|
29.2
|
1.0
|
OD1
|
B:ASP232
|
3.7
|
40.3
|
1.0
|
N
|
B:HIS126
|
4.0
|
36.7
|
1.0
|
O11
|
B:2BH1001
|
4.1
|
28.1
|
1.0
|
C09
|
B:2BH1001
|
4.1
|
28.4
|
1.0
|
NE2
|
B:HIS126
|
4.2
|
32.4
|
1.0
|
CB
|
B:ASP234
|
4.2
|
33.4
|
1.0
|
CB
|
B:ASP232
|
4.2
|
38.0
|
1.0
|
N
|
B:ALA125
|
4.2
|
34.7
|
1.0
|
C08
|
B:2BH1001
|
4.2
|
28.8
|
1.0
|
CD2
|
B:HIS126
|
4.3
|
29.9
|
1.0
|
O
|
B:HOH739
|
4.3
|
20.9
|
1.0
|
OD1
|
B:ASP128
|
4.4
|
28.4
|
1.0
|
CA
|
B:HIS126
|
4.4
|
35.2
|
1.0
|
CB
|
B:ALA125
|
4.6
|
35.9
|
1.0
|
CB
|
B:ASP124
|
4.6
|
36.5
|
1.0
|
OD2
|
B:ASP128
|
4.6
|
22.6
|
1.0
|
C
|
B:ALA125
|
4.8
|
34.3
|
1.0
|
CA
|
B:ALA125
|
4.9
|
34.0
|
1.0
|
O
|
B:HIS126
|
4.9
|
35.2
|
1.0
|
CG
|
B:ASP128
|
5.0
|
26.7
|
1.0
|
|
Manganese binding site 4 out
of 6 in 1t4p
Go back to
Manganese Binding Sites List in 1t4p
Manganese binding site 4 out
of 6 in the Arginase-Dehydro-Abh Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 4 of Arginase-Dehydro-Abh Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mn501
b:30.5
occ:1.00
|
OD2
|
B:ASP232
|
2.1
|
40.9
|
1.0
|
O12
|
B:2BH1001
|
2.2
|
27.5
|
1.0
|
ND1
|
B:HIS101
|
2.3
|
25.5
|
1.0
|
OD2
|
B:ASP128
|
2.3
|
22.6
|
1.0
|
OD2
|
B:ASP124
|
2.4
|
32.0
|
1.0
|
O11
|
B:2BH1001
|
2.5
|
28.1
|
1.0
|
B
|
B:2BH1001
|
2.9
|
29.6
|
1.0
|
CG
|
B:ASP232
|
3.1
|
40.3
|
1.0
|
CG
|
B:HIS101
|
3.2
|
27.3
|
1.0
|
MN
|
B:MN500
|
3.3
|
27.8
|
1.0
|
CE1
|
B:HIS101
|
3.3
|
25.2
|
1.0
|
CG
|
B:ASP128
|
3.3
|
26.7
|
1.0
|
CB
|
B:HIS101
|
3.3
|
32.5
|
1.0
|
CG
|
B:ASP124
|
3.4
|
35.6
|
1.0
|
CB
|
B:ASP232
|
3.6
|
38.0
|
1.0
|
OD1
|
B:ASP128
|
3.6
|
28.4
|
1.0
|
OD1
|
B:ASP124
|
3.7
|
38.6
|
1.0
|
O13
|
B:2BH1001
|
3.7
|
32.8
|
1.0
|
C09
|
B:2BH1001
|
4.0
|
28.4
|
1.0
|
OD1
|
B:ASP232
|
4.3
|
40.3
|
1.0
|
CD2
|
B:HIS101
|
4.3
|
25.5
|
1.0
|
NE2
|
B:HIS101
|
4.4
|
25.5
|
1.0
|
OE2
|
B:GLU277
|
4.4
|
44.5
|
1.0
|
O
|
B:HIS141
|
4.5
|
34.6
|
1.0
|
CG
|
B:GLU277
|
4.5
|
42.6
|
1.0
|
NE1
|
B:TRP122
|
4.5
|
22.5
|
1.0
|
CB
|
B:ASP128
|
4.6
|
31.2
|
1.0
|
CZ2
|
B:TRP122
|
4.7
|
21.2
|
1.0
|
OD2
|
B:ASP234
|
4.7
|
37.5
|
1.0
|
CB
|
B:ASP124
|
4.7
|
36.5
|
1.0
|
CA
|
B:HIS101
|
4.9
|
42.2
|
1.0
|
CA
|
B:ASP232
|
4.9
|
34.7
|
1.0
|
C08
|
B:2BH1001
|
4.9
|
28.8
|
1.0
|
CE2
|
B:TRP122
|
5.0
|
22.4
|
1.0
|
CD
|
B:GLU277
|
5.0
|
45.1
|
1.0
|
ND1
|
B:HIS126
|
5.0
|
31.1
|
1.0
|
|
Manganese binding site 5 out
of 6 in 1t4p
Go back to
Manganese Binding Sites List in 1t4p
Manganese binding site 5 out
of 6 in the Arginase-Dehydro-Abh Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 5 of Arginase-Dehydro-Abh Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mn503
b:42.3
occ:1.00
|
OD2
|
C:ASP232
|
2.1
|
40.9
|
1.0
|
O12
|
C:2BH1002
|
2.2
|
27.5
|
1.0
|
OD2
|
C:ASP124
|
2.2
|
38.5
|
1.0
|
ND1
|
C:HIS101
|
2.3
|
42.0
|
1.0
|
OD2
|
C:ASP128
|
2.3
|
37.7
|
1.0
|
O11
|
C:2BH1002
|
2.5
|
28.1
|
1.0
|
B
|
C:2BH1002
|
2.9
|
29.6
|
1.0
|
CG
|
C:ASP232
|
3.2
|
40.9
|
1.0
|
CG
|
C:HIS101
|
3.2
|
41.8
|
1.0
|
CG
|
C:ASP128
|
3.2
|
38.8
|
1.0
|
MN
|
C:MN505
|
3.3
|
44.6
|
1.0
|
CG
|
C:ASP124
|
3.3
|
40.5
|
1.0
|
CE1
|
C:HIS101
|
3.3
|
40.9
|
1.0
|
CB
|
C:HIS101
|
3.4
|
44.4
|
1.0
|
OD1
|
C:ASP128
|
3.5
|
41.8
|
1.0
|
CB
|
C:ASP232
|
3.6
|
40.5
|
1.0
|
OD1
|
C:ASP124
|
3.6
|
40.4
|
1.0
|
O13
|
C:2BH1002
|
3.7
|
32.8
|
1.0
|
C09
|
C:2BH1002
|
4.0
|
28.4
|
1.0
|
OD1
|
C:ASP232
|
4.3
|
41.3
|
1.0
|
CD2
|
C:HIS101
|
4.4
|
41.0
|
1.0
|
NE2
|
C:HIS101
|
4.4
|
41.7
|
1.0
|
NE1
|
C:TRP122
|
4.4
|
34.2
|
1.0
|
O
|
C:HIS141
|
4.4
|
46.8
|
1.0
|
OE2
|
C:GLU277
|
4.5
|
53.3
|
1.0
|
OD2
|
C:ASP234
|
4.6
|
37.9
|
1.0
|
CB
|
C:ASP128
|
4.6
|
39.4
|
1.0
|
CG
|
C:GLU277
|
4.6
|
50.8
|
1.0
|
CB
|
C:ASP124
|
4.6
|
40.6
|
1.0
|
CZ2
|
C:TRP122
|
4.7
|
32.6
|
1.0
|
CA
|
C:ASP232
|
4.9
|
41.6
|
1.0
|
CE2
|
C:TRP122
|
4.9
|
33.6
|
1.0
|
C08
|
C:2BH1002
|
4.9
|
28.8
|
1.0
|
CA
|
C:HIS101
|
4.9
|
48.2
|
1.0
|
|
Manganese binding site 6 out
of 6 in 1t4p
Go back to
Manganese Binding Sites List in 1t4p
Manganese binding site 6 out
of 6 in the Arginase-Dehydro-Abh Complex
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 6 of Arginase-Dehydro-Abh Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mn505
b:44.6
occ:1.00
|
O12
|
C:2BH1002
|
2.0
|
27.5
|
1.0
|
OD2
|
C:ASP234
|
2.1
|
37.9
|
1.0
|
ND1
|
C:HIS126
|
2.2
|
45.1
|
1.0
|
OD1
|
C:ASP124
|
2.3
|
40.4
|
1.0
|
OD2
|
C:ASP232
|
2.5
|
40.9
|
1.0
|
OD1
|
C:ASP234
|
2.5
|
38.6
|
1.0
|
CG
|
C:ASP234
|
2.6
|
38.5
|
1.0
|
CE1
|
C:HIS126
|
3.1
|
43.9
|
1.0
|
CG
|
C:ASP124
|
3.2
|
40.5
|
1.0
|
O13
|
C:2BH1002
|
3.2
|
32.8
|
1.0
|
B
|
C:2BH1002
|
3.2
|
29.6
|
1.0
|
CG
|
C:ASP232
|
3.2
|
40.9
|
1.0
|
MN
|
C:MN503
|
3.3
|
42.3
|
1.0
|
CG
|
C:HIS126
|
3.3
|
44.0
|
1.0
|
OD2
|
C:ASP124
|
3.4
|
38.5
|
1.0
|
OD1
|
C:ASP232
|
3.7
|
41.3
|
1.0
|
CB
|
C:HIS126
|
3.7
|
41.9
|
1.0
|
N
|
C:HIS126
|
4.0
|
40.5
|
1.0
|
O11
|
C:2BH1002
|
4.1
|
28.1
|
1.0
|
CB
|
C:ASP234
|
4.1
|
37.2
|
1.0
|
C09
|
C:2BH1002
|
4.1
|
28.4
|
1.0
|
CB
|
C:ASP232
|
4.2
|
40.5
|
1.0
|
N
|
C:ALA125
|
4.2
|
38.1
|
1.0
|
C08
|
C:2BH1002
|
4.2
|
28.8
|
1.0
|
NE2
|
C:HIS126
|
4.3
|
43.2
|
1.0
|
OD1
|
C:ASP128
|
4.3
|
41.8
|
1.0
|
CD2
|
C:HIS126
|
4.4
|
41.8
|
1.0
|
CA
|
C:HIS126
|
4.5
|
40.3
|
1.0
|
CB
|
C:ASP124
|
4.6
|
40.6
|
1.0
|
O
|
C:HOH774
|
4.6
|
29.0
|
1.0
|
CB
|
C:ALA125
|
4.7
|
40.4
|
1.0
|
OD2
|
C:ASP128
|
4.8
|
37.7
|
1.0
|
C
|
C:ALA125
|
4.8
|
39.6
|
1.0
|
CA
|
C:ALA125
|
4.9
|
38.5
|
1.0
|
O
|
C:HIS126
|
5.0
|
41.0
|
1.0
|
|
Reference:
E.Cama,
S.Pethe,
J.-L.Boucher,
S.Han,
F.A.Emig,
D.E.Ash,
R.E.Viola,
D.Mansuy,
D.W.Christianson.
Inhibitor Coordination Interactions in the Binuclear Manganese Cluster of Arginase Biochemistry V. 43 8987 2004.
ISSN: ISSN 0006-2960
PubMed: 15248756
DOI: 10.1021/BI0491705
Page generated: Sat Oct 5 12:27:38 2024
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