Manganese in PDB 1s95: Structure of Serine/Threonine Protein Phosphatase 5
Enzymatic activity of Structure of Serine/Threonine Protein Phosphatase 5
All present enzymatic activity of Structure of Serine/Threonine Protein Phosphatase 5:
3.1.3.16;
Protein crystallography data
The structure of Structure of Serine/Threonine Protein Phosphatase 5, PDB code: 1s95
was solved by
M.R.Swingle,
R.E.Honkanen,
E.M.Ciszak,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
40.00 /
1.60
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
40.808,
80.309,
92.189,
90.00,
94.25,
90.00
|
R / Rfree (%)
|
16.9 /
20.9
|
Manganese Binding Sites:
The binding sites of Manganese atom in the Structure of Serine/Threonine Protein Phosphatase 5
(pdb code 1s95). This binding sites where shown within
5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the
Structure of Serine/Threonine Protein Phosphatase 5, PDB code: 1s95:
Jump to Manganese binding site number:
1;
2;
3;
4;
Manganese binding site 1 out
of 4 in 1s95
Go back to
Manganese Binding Sites List in 1s95
Manganese binding site 1 out
of 4 in the Structure of Serine/Threonine Protein Phosphatase 5
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 1 of Structure of Serine/Threonine Protein Phosphatase 5 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn501
b:24.4
occ:1.00
|
OD2
|
A:ASP242
|
2.1
|
25.9
|
1.0
|
O
|
A:HOH3002
|
2.1
|
24.0
|
1.0
|
NE2
|
A:HIS244
|
2.2
|
28.8
|
1.0
|
OD2
|
A:ASP271
|
2.2
|
26.3
|
1.0
|
O1
|
A:PO41001
|
2.2
|
27.0
|
1.0
|
O
|
A:HOH3003
|
2.3
|
22.6
|
1.0
|
CE1
|
A:HIS244
|
3.1
|
26.5
|
1.0
|
CD2
|
A:HIS244
|
3.2
|
29.9
|
1.0
|
CG
|
A:ASP271
|
3.2
|
26.8
|
1.0
|
MN
|
A:MN502
|
3.2
|
21.6
|
1.0
|
P
|
A:PO41001
|
3.3
|
28.6
|
1.0
|
CG
|
A:ASP242
|
3.3
|
30.3
|
1.0
|
O3
|
A:PO41001
|
3.6
|
29.2
|
1.0
|
O2
|
A:PO41001
|
3.7
|
28.0
|
1.0
|
CB
|
A:ASP271
|
3.7
|
24.9
|
1.0
|
O
|
A:HOH3175
|
4.0
|
39.3
|
1.0
|
CB
|
A:ASP242
|
4.1
|
26.1
|
1.0
|
OD1
|
A:ASP242
|
4.2
|
28.4
|
1.0
|
NH1
|
A:ARG275
|
4.2
|
32.4
|
1.0
|
ND1
|
A:HIS244
|
4.3
|
27.2
|
1.0
|
CD2
|
A:HIS304
|
4.3
|
27.2
|
1.0
|
CG
|
A:HIS244
|
4.3
|
27.1
|
1.0
|
OD1
|
A:ASP271
|
4.3
|
27.5
|
1.0
|
OH
|
A:TYR451
|
4.4
|
31.2
|
1.0
|
CE1
|
A:PHE446
|
4.4
|
29.7
|
1.0
|
O
|
A:HIS427
|
4.4
|
28.8
|
1.0
|
NE2
|
A:HIS352
|
4.5
|
24.1
|
1.0
|
CE1
|
A:HIS352
|
4.5
|
28.7
|
1.0
|
CA
|
A:HIS427
|
4.6
|
26.9
|
1.0
|
O4
|
A:PO41001
|
4.7
|
27.3
|
1.0
|
C
|
A:HIS427
|
4.7
|
27.6
|
1.0
|
NE2
|
A:HIS304
|
4.8
|
26.6
|
1.0
|
OD1
|
A:ASN303
|
4.9
|
29.8
|
1.0
|
ND1
|
A:HIS427
|
5.0
|
26.3
|
1.0
|
|
Manganese binding site 2 out
of 4 in 1s95
Go back to
Manganese Binding Sites List in 1s95
Manganese binding site 2 out
of 4 in the Structure of Serine/Threonine Protein Phosphatase 5
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 2 of Structure of Serine/Threonine Protein Phosphatase 5 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mn502
b:21.6
occ:1.00
|
O
|
A:HOH3002
|
2.1
|
24.0
|
1.0
|
OD1
|
A:ASN303
|
2.1
|
29.8
|
1.0
|
ND1
|
A:HIS427
|
2.2
|
26.3
|
1.0
|
NE2
|
A:HIS352
|
2.3
|
24.1
|
1.0
|
O2
|
A:PO41001
|
2.3
|
28.0
|
1.0
|
OD2
|
A:ASP271
|
2.3
|
26.3
|
1.0
|
CG
|
A:ASN303
|
3.1
|
29.1
|
1.0
|
CE1
|
A:HIS427
|
3.1
|
28.6
|
1.0
|
CD2
|
A:HIS352
|
3.2
|
28.8
|
1.0
|
CG
|
A:ASP271
|
3.2
|
26.8
|
1.0
|
MN
|
A:MN501
|
3.2
|
24.4
|
1.0
|
CE1
|
A:HIS352
|
3.2
|
28.7
|
1.0
|
CG
|
A:HIS427
|
3.3
|
26.5
|
1.0
|
P
|
A:PO41001
|
3.4
|
28.6
|
1.0
|
ND2
|
A:ASN303
|
3.5
|
28.0
|
1.0
|
OD1
|
A:ASP271
|
3.6
|
27.5
|
1.0
|
O1
|
A:PO41001
|
3.6
|
27.0
|
1.0
|
CA
|
A:HIS427
|
3.7
|
26.9
|
1.0
|
CB
|
A:HIS427
|
3.7
|
26.7
|
1.0
|
O3
|
A:PO41001
|
3.9
|
29.2
|
1.0
|
OD2
|
A:ASP242
|
4.0
|
25.9
|
1.0
|
O
|
A:HIS427
|
4.1
|
28.8
|
1.0
|
NE2
|
A:HIS427
|
4.3
|
25.0
|
1.0
|
CD2
|
A:HIS304
|
4.3
|
27.2
|
1.0
|
C
|
A:HIS427
|
4.3
|
27.6
|
1.0
|
ND1
|
A:HIS352
|
4.4
|
23.3
|
1.0
|
CD2
|
A:HIS427
|
4.4
|
24.7
|
1.0
|
CG
|
A:HIS352
|
4.4
|
26.1
|
1.0
|
CB
|
A:ASN303
|
4.4
|
26.6
|
1.0
|
N
|
A:ASN303
|
4.4
|
26.5
|
1.0
|
CB
|
A:ASP271
|
4.5
|
24.9
|
1.0
|
O4
|
A:PO41001
|
4.7
|
27.3
|
1.0
|
O
|
A:LEU385
|
4.7
|
29.1
|
1.0
|
N
|
A:HIS427
|
4.8
|
26.4
|
1.0
|
NH1
|
A:ARG400
|
4.8
|
32.9
|
1.0
|
CA
|
A:ASN303
|
4.9
|
25.2
|
1.0
|
CG
|
A:ASP242
|
5.0
|
30.3
|
1.0
|
|
Manganese binding site 3 out
of 4 in 1s95
Go back to
Manganese Binding Sites List in 1s95
Manganese binding site 3 out
of 4 in the Structure of Serine/Threonine Protein Phosphatase 5
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 3 of Structure of Serine/Threonine Protein Phosphatase 5 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mn601
b:19.0
occ:1.00
|
O
|
B:HOH3003
|
2.0
|
17.9
|
1.0
|
OD2
|
B:ASP242
|
2.1
|
25.0
|
1.0
|
NE2
|
B:HIS244
|
2.2
|
26.5
|
1.0
|
O1
|
B:PO42001
|
2.2
|
20.0
|
1.0
|
OD2
|
B:ASP271
|
2.2
|
24.9
|
1.0
|
O
|
B:HOH3004
|
2.4
|
16.6
|
1.0
|
CE1
|
B:HIS244
|
3.1
|
27.6
|
1.0
|
CD2
|
B:HIS244
|
3.2
|
27.4
|
1.0
|
MN
|
B:MN602
|
3.2
|
14.6
|
1.0
|
CG
|
B:ASP271
|
3.3
|
28.9
|
1.0
|
P
|
B:PO42001
|
3.3
|
21.1
|
1.0
|
CG
|
B:ASP242
|
3.3
|
29.6
|
1.0
|
CB
|
B:ASP271
|
3.7
|
26.3
|
1.0
|
O2
|
B:PO42001
|
3.7
|
21.5
|
1.0
|
O3
|
B:PO42001
|
3.9
|
21.6
|
1.0
|
CB
|
B:ASP242
|
4.1
|
24.9
|
1.0
|
OD1
|
B:ASP242
|
4.2
|
27.2
|
1.0
|
ND1
|
B:HIS244
|
4.2
|
24.6
|
1.0
|
CD2
|
B:HIS304
|
4.3
|
27.8
|
1.0
|
CG
|
B:HIS244
|
4.3
|
23.6
|
1.0
|
OD1
|
B:ASP271
|
4.3
|
24.6
|
1.0
|
O
|
B:HOH3087
|
4.3
|
32.4
|
1.0
|
NH1
|
B:ARG275
|
4.3
|
34.9
|
1.0
|
CE1
|
B:HIS352
|
4.3
|
25.4
|
1.0
|
NE2
|
B:HIS352
|
4.4
|
27.9
|
1.0
|
OH
|
B:TYR451
|
4.4
|
29.4
|
1.0
|
O
|
B:HIS427
|
4.4
|
27.5
|
1.0
|
CE1
|
B:PHE446
|
4.5
|
26.5
|
1.0
|
O4
|
B:PO42001
|
4.6
|
21.2
|
1.0
|
CA
|
B:HIS427
|
4.7
|
25.8
|
1.0
|
NE2
|
B:HIS304
|
4.7
|
24.1
|
1.0
|
C
|
B:HIS427
|
4.8
|
26.0
|
1.0
|
OD1
|
B:ASN303
|
4.9
|
25.1
|
1.0
|
ND1
|
B:HIS427
|
4.9
|
24.8
|
1.0
|
|
Manganese binding site 4 out
of 4 in 1s95
Go back to
Manganese Binding Sites List in 1s95
Manganese binding site 4 out
of 4 in the Structure of Serine/Threonine Protein Phosphatase 5
Mono view
Stereo pair view
|
A full contact list of Manganese with other atoms in the Mn binding
site number 4 of Structure of Serine/Threonine Protein Phosphatase 5 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mn602
b:14.6
occ:1.00
|
NE2
|
B:HIS352
|
2.1
|
27.9
|
1.0
|
OD1
|
B:ASN303
|
2.2
|
25.1
|
1.0
|
O
|
B:HOH3003
|
2.2
|
17.9
|
1.0
|
O2
|
B:PO42001
|
2.2
|
21.5
|
1.0
|
ND1
|
B:HIS427
|
2.3
|
24.8
|
1.0
|
OD2
|
B:ASP271
|
2.4
|
24.9
|
1.0
|
CE1
|
B:HIS427
|
3.1
|
24.9
|
1.0
|
CE1
|
B:HIS352
|
3.1
|
25.4
|
1.0
|
CG
|
B:ASN303
|
3.1
|
25.6
|
1.0
|
CD2
|
B:HIS352
|
3.2
|
27.4
|
1.0
|
MN
|
B:MN601
|
3.2
|
19.0
|
1.0
|
CG
|
B:ASP271
|
3.2
|
28.9
|
1.0
|
P
|
B:PO42001
|
3.3
|
21.1
|
1.0
|
CG
|
B:HIS427
|
3.4
|
26.6
|
1.0
|
OD1
|
B:ASP271
|
3.5
|
24.6
|
1.0
|
ND2
|
B:ASN303
|
3.5
|
25.7
|
1.0
|
O1
|
B:PO42001
|
3.6
|
20.0
|
1.0
|
CA
|
B:HIS427
|
3.8
|
25.8
|
1.0
|
CB
|
B:HIS427
|
3.9
|
27.0
|
1.0
|
OD2
|
B:ASP242
|
4.0
|
25.0
|
1.0
|
O3
|
B:PO42001
|
4.1
|
21.6
|
1.0
|
O
|
B:HIS427
|
4.2
|
27.5
|
1.0
|
CD2
|
B:HIS304
|
4.2
|
27.8
|
1.0
|
ND1
|
B:HIS352
|
4.2
|
25.6
|
1.0
|
NE2
|
B:HIS427
|
4.3
|
23.9
|
1.0
|
CG
|
B:HIS352
|
4.3
|
25.1
|
1.0
|
N
|
B:ASN303
|
4.4
|
22.6
|
1.0
|
CD2
|
B:HIS427
|
4.4
|
24.7
|
1.0
|
CB
|
B:ASP271
|
4.5
|
26.3
|
1.0
|
CB
|
B:ASN303
|
4.5
|
25.2
|
1.0
|
C
|
B:HIS427
|
4.5
|
26.0
|
1.0
|
O4
|
B:PO42001
|
4.6
|
21.2
|
1.0
|
O
|
B:LEU385
|
4.7
|
25.3
|
1.0
|
N
|
B:HIS427
|
4.8
|
26.3
|
1.0
|
NE2
|
B:HIS304
|
4.9
|
24.1
|
1.0
|
CA
|
B:ASN303
|
5.0
|
24.2
|
1.0
|
NH1
|
B:ARG400
|
5.0
|
31.3
|
1.0
|
CG
|
B:ASP242
|
5.0
|
29.6
|
1.0
|
|
Reference:
M.R.Swingle,
R.E.Honkanen,
E.M.Ciszak.
Structural Basis For the Catalytic Activity of Human Serine/Threonine Protein Phosphatase-5. J.Biol.Chem. V. 279 33992 2004.
ISSN: ISSN 0021-9258
PubMed: 15155720
DOI: 10.1074/JBC.M402855200
Page generated: Sat Oct 5 12:24:24 2024
|