Manganese in PDB 1s4o: Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn

All present enzymatic activity of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn:
2.4.1.131;

The structure of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn, PDB code: 1s4o was solved by Y.D.Lobsanov, P.A.Romero, B.Sleno, B.Yu, P.Yip, A.Herscovics, P.L.Howell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	52.42 / 2.01
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	60.694, 101.068, 62.036, 90.00, 98.81, 90.00
R / Rfree (%)	19.7 / 25

The structure of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn also contains other interesting chemical elements:

Chlorine

(Cl)

4 atoms

The binding sites of Manganese atom in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn (pdb code 1s4o). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn, PDB code: 1s4o:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn601 b:21.9 occ:1.00 O1A A:GDP800 2.2 21.7 1.0 OE1 A:GLU247 2.2 19.9 1.0 O2B A:GDP800 2.3 22.8 1.0 O A:HOH1151 2.3 23.9 1.0 NE2 A:HIS388 2.3 12.8 1.0 O A:HOH928 2.5 21.9 1.0 CE1 A:HIS388 3.3 17.1 1.0 CD2 A:HIS388 3.3 17.4 1.0 PB A:GDP800 3.3 25.1 1.0 PA A:GDP800 3.4 25.1 1.0 CD A:GLU247 3.4 19.9 1.0 O3A A:GDP800 3.6 23.0 1.0 O3B A:GDP800 3.7 22.1 1.0 O A:HOH959 3.9 30.4 1.0 O A:HOH957 4.0 21.1 1.0 OE2 A:GLU247 4.1 21.1 1.0 O A:HOH1019 4.2 25.1 1.0 C5' A:GDP800 4.3 20.3 1.0 O5' A:GDP800 4.3 21.3 1.0 ND1 A:HIS388 4.4 16.4 1.0 CB A:GLU247 4.4 16.1 1.0 CG A:HIS388 4.4 15.3 1.0 O2A A:GDP800 4.5 23.8 1.0 CG A:GLU247 4.5 18.7 1.0 O A:HOH1041 4.6 31.5 1.0 O1B A:GDP800 4.7 24.2 1.0 C3' A:GDP800 4.7 20.7 1.0 O A:HOH983 4.8 37.0 1.0 CB A:ASN327 4.9 13.4 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Yeast ALPHA1,2-Mannosyltransferase KRE2P/MNT1P: Binary Complex with Gdp/Mn within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn602 b:23.1 occ:1.00 OE1 B:GLU247 2.1 22.7 1.0 O1A B:GDP801 2.2 18.1 1.0 O B:HOH1137 2.2 24.6 1.0 O2B B:GDP801 2.3 23.6 1.0 NE2 B:HIS388 2.3 12.1 1.0 O B:HOH1136 2.3 18.2 1.0 CE1 B:HIS388 3.2 14.6 1.0 PB B:GDP801 3.3 23.2 1.0 CD B:GLU247 3.4 24.1 1.0 CD2 B:HIS388 3.4 13.7 1.0 PA B:GDP801 3.4 23.0 1.0 O3B B:GDP801 3.6 25.1 1.0 O3A B:GDP801 3.6 21.5 1.0 O B:HOH976 3.8 22.4 1.0 O B:HOH1004 3.9 22.7 1.0 OE2 B:GLU247 4.0 25.4 1.0 O B:HOH1020 4.1 28.6 1.0 C5' B:GDP801 4.2 22.3 1.0 O5' B:GDP801 4.3 21.2 1.0 ND1 B:HIS388 4.3 14.3 1.0 CG B:GLU247 4.5 23.7 1.0 CG B:HIS388 4.5 13.6 1.0 CB B:GLU247 4.5 19.4 1.0 O A:HOH1148 4.6 28.6 1.0 O2A B:GDP801 4.6 22.6 1.0 O1B B:GDP801 4.7 25.3 1.0 C3' B:GDP801 4.8 23.0 1.0 O B:HOH1087 4.8 27.7 1.0 O B:HOH971 5.0 30.6 1.0 C4' B:GDP801 5.0 22.5 1.0

Y.D.Lobsanov, P.A.Romero, B.Sleno, B.Yu, P.Yip, A.Herscovics, P.L.Howell. Structure of KRE2P/MNT1P: A Yeast {Alpha}1,2-Mannosyltransferase Involved in Mannoprotein Biosynthesis J.Biol.Chem. V. 279 17921 2004.
ISSN: ISSN 0021-9258
PubMed: 14752117
DOI: 10.1074/JBC.M312720200