Manganese in PDB 1r8b: Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide

All present enzymatic activity of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide:
2.7.7.25;

The structure of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide, PDB code: 1r8b was solved by Y.Xiong, F.Li, J.Wang, A.M.Weiner, T.A.Steitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.63 / 2.00
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	86.402, 79.371, 78.135, 90.00, 97.33, 90.00
R / Rfree (%)	18.1 / 22.8

The structure of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Chlorine	(Cl)	1 atom
Sodium	(Na)	1 atom

The binding sites of Manganese atom in the Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide (pdb code 1r8b). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide, PDB code: 1r8b:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn600 b:36.3 occ:1.00 O3G A:ATP501 2.2 38.3 1.0 O2B A:ATP501 2.2 30.3 1.0 O1A A:ATP501 2.3 29.8 1.0 OD2 A:ASP61 2.5 28.6 1.0 MG A:MG604 2.6 62.1 1.0 PB A:ATP501 3.2 34.6 1.0 PG A:ATP501 3.4 35.1 1.0 CG A:ASP61 3.4 28.6 1.0 PA A:ATP501 3.4 37.9 1.0 O3B A:ATP501 3.6 35.1 1.0 OD1 A:ASP61 3.6 29.4 1.0 O A:HOH778 3.7 48.1 0.5 O3A A:ATP501 3.7 39.1 1.0 OG A:SER47 4.1 26.5 1.0 O1G A:ATP501 4.1 40.4 1.0 N A:SER47 4.3 22.7 1.0 O A:HOH750 4.4 70.7 1.0 O5' A:ATP501 4.4 45.3 1.0 C5' A:ATP501 4.5 44.8 1.0 O1B A:ATP501 4.5 34.1 1.0 O2A A:ATP501 4.6 40.4 1.0 O2G A:ATP501 4.6 34.8 1.0 OE1 A:GLU59 4.6 36.0 1.0 O A:GLU59 4.7 27.2 1.0 CB A:SER47 4.8 21.7 1.0 CB A:GLU59 4.8 31.7 1.0 CB A:ASP61 4.8 27.6 1.0 CA A:GLY46 4.9 25.7 1.0

Manganese binding site 2 out of 2 in the Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn601 b:27.4 occ:1.00 O1B A:ATP502 2.0 24.8 1.0 NE2 A:HIS398 2.1 33.1 1.0 ND1 A:HIS396 2.2 18.0 1.0 O3G A:ATP502 2.3 36.1 1.0 CD2 A:HIS398 3.1 30.7 1.0 CE1 A:HIS396 3.1 18.8 1.0 PB A:ATP502 3.1 32.1 1.0 CE1 A:HIS398 3.2 34.7 1.0 PG A:ATP502 3.3 35.8 1.0 CG A:HIS396 3.3 11.7 1.0 O3B A:ATP502 3.5 30.5 1.0 CB A:HIS396 3.7 10.5 1.0 O3A A:ATP502 3.7 26.7 1.0 O2G A:ATP502 3.7 36.5 1.0 CA A:HIS396 3.8 10.8 1.0 NE2 A:HIS396 4.2 16.3 1.0 CG A:HIS398 4.2 24.2 1.0 ND1 A:HIS398 4.3 33.2 1.0 O A:THR395 4.3 12.8 1.0 CD2 A:HIS396 4.3 16.5 1.0 O2B A:ATP502 4.5 36.7 1.0 O1G A:ATP502 4.6 40.1 1.0 N A:HIS396 4.8 9.1 1.0 C A:HIS396 4.8 11.4 1.0 C A:THR395 4.9 11.4 1.0 C5' A:ATP502 4.9 33.8 1.0 O A:HIS396 4.9 12.1 1.0

Y.Xiong, F.Li, J.Wang, A.M.Weiner, T.A.Steitz. Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide Complexes Mol.Cell V. 12 1165 2003.
ISSN: ISSN 1097-2765
PubMed: 14636575
DOI: 10.1016/S1097-2765(03)00440-4