Manganese in PDB 1on8: Crystal Structure of Mouse Alpha-1,4-N-Acetylhexosaminyltransferase (EXTL2) with Udp and Glcuab(1-3)Galb(1-O)-Naphthalenelmethanol An Acceptor Substrate Analog

The structure of Crystal Structure of Mouse Alpha-1,4-N-Acetylhexosaminyltransferase (EXTL2) with Udp and Glcuab(1-3)Galb(1-O)-Naphthalenelmethanol An Acceptor Substrate Analog, PDB code: 1on8 was solved by L.C.Pedersen, J.Dong, F.Taniguchi, H.Kitagawa, J.M.Krahn, L.G.Pedersen, K.Sugahara, M.Negishi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.98 / 2.70
Space group	P 4 21 2
Cell size a, b, c (Å), α, β, γ (°)	125.157, 125.157, 83.692, 90.00, 90.00, 90.00
R / Rfree (%)	19.2 / 25.4

The binding sites of Manganese atom in the Crystal Structure of Mouse Alpha-1,4-N-Acetylhexosaminyltransferase (EXTL2) with Udp and Glcuab(1-3)Galb(1-O)-Naphthalenelmethanol An Acceptor Substrate Analog (pdb code 1on8). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Mouse Alpha-1,4-N-Acetylhexosaminyltransferase (EXTL2) with Udp and Glcuab(1-3)Galb(1-O)-Naphthalenelmethanol An Acceptor Substrate Analog, PDB code: 1on8:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Mouse Alpha-1,4-N-Acetylhexosaminyltransferase (EXTL2) with Udp and Glcuab(1-3)Galb(1-O)-Naphthalenelmethanol An Acceptor Substrate Analog


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Mouse Alpha-1,4-N-Acetylhexosaminyltransferase (EXTL2) with Udp and Glcuab(1-3)Galb(1-O)-Naphthalenelmethanol An Acceptor Substrate Analog within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn600 b:37.4 occ:1.00 O3B A:UDP351 2.0 35.3 1.0 O A:HOH605 2.1 26.2 1.0 O2A A:UDP351 2.2 26.4 1.0 O A:HOH604 2.2 20.6 1.0 O A:HOH606 2.2 24.7 1.0 OD2 A:ASP153 2.6 20.3 1.0 PB A:UDP351 3.1 32.4 1.0 PA A:UDP351 3.2 27.1 1.0 CG A:ASP153 3.3 19.3 1.0 O3A A:UDP351 3.3 30.1 1.0 OD1 A:ASP153 3.4 17.0 1.0 O2B A:UDP351 3.4 32.3 1.0 O1A A:UDP351 4.0 29.2 1.0 O3 A:BDP509 4.1 35.9 1.0 NH2 A:ARG76 4.1 19.8 1.0 OD2 A:ASP151 4.3 26.8 1.0 OE2 A:GLU274 4.3 48.3 1.0 NH1 A:ARG76 4.3 16.9 1.0 CG A:GLU274 4.4 44.0 1.0 CD A:GLU274 4.4 45.5 1.0 ND2 A:ASN272 4.4 26.9 1.0 O5' A:UDP351 4.5 25.5 1.0 CD1 A:TRP284 4.6 40.7 1.0 O1B A:UDP351 4.6 32.5 1.0 CB A:ASP153 4.6 18.4 1.0 CZ A:ARG76 4.7 19.1 1.0 O2 A:BDP509 4.7 38.4 1.0 CB A:ASP151 4.8 19.3 1.0 C3 A:BDP509 4.8 37.7 1.0 C5' A:UDP351 4.8 25.7 1.0 CG A:ASP151 5.0 22.5 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Mouse Alpha-1,4-N-Acetylhexosaminyltransferase (EXTL2) with Udp and Glcuab(1-3)Galb(1-O)-Naphthalenelmethanol An Acceptor Substrate Analog


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Mouse Alpha-1,4-N-Acetylhexosaminyltransferase (EXTL2) with Udp and Glcuab(1-3)Galb(1-O)-Naphthalenelmethanol An Acceptor Substrate Analog within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn601 b:41.9 occ:1.00 O3B B:UDP352 2.0 35.4 1.0 O B:HOH613 2.2 32.7 1.0 O B:HOH614 2.2 28.1 1.0 O2A B:UDP352 2.4 31.7 1.0 OD2 B:ASP153 2.4 26.0 1.0 O B:HOH615 2.5 29.9 1.0 PB B:UDP352 3.1 35.7 1.0 CG B:ASP153 3.2 25.1 1.0 OD1 B:ASP153 3.3 24.4 1.0 PA B:UDP352 3.4 32.2 1.0 O3A B:UDP352 3.4 33.7 1.0 O2B B:UDP352 3.5 32.9 1.0 OE2 B:GLU274 3.9 55.0 1.0 NH2 B:ARG76 4.0 24.6 1.0 O3 B:BDP519 4.2 49.8 1.0 O1A B:UDP352 4.2 34.3 1.0 ND2 B:ASN272 4.2 31.0 1.0 NH1 B:ARG76 4.2 25.6 1.0 OD2 B:ASP151 4.3 29.1 1.0 CB B:ASP153 4.5 24.9 1.0 O2 B:BDP519 4.6 50.1 1.0 CZ B:ARG76 4.6 25.8 1.0 O1B B:UDP352 4.6 34.5 1.0 O5' B:UDP352 4.7 32.9 1.0 CD B:GLU274 4.7 52.3 1.0 CB B:ASP151 4.7 25.5 1.0 CD1 B:TRP284 4.7 42.4 1.0 CG B:ASP151 4.9 27.3 1.0 C3 B:BDP519 4.9 49.1 1.0 C5' B:UDP352 5.0 30.5 1.0 CG B:GLU274 5.0 49.8 1.0

L.C.Pedersen, J.Dong, F.Taniguchi, H.Kitagawa, J.M.Krahn, L.G.Pedersen, K.Sugahara, M.Negishi. Crystal Structure of An Alpha-1,4-N-Acetylhexosaminyltransferase (EXTL2), A Member of the Exostosin Gene Family Involved in Heparan Sulfate Biosynthesis J.Biol.Chem. V. 278 14420 2003.
ISSN: ISSN 0021-9258
PubMed: 12562774
DOI: 10.1074/JBC.M210532200