Manganese in PDB 1ols: Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase

Enzymatic activity of Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase

All present enzymatic activity of Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase:
1.2.4.4;

Protein crystallography data

The structure of Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase, PDB code: 1ols was solved by R.M.Wynn, M.Machius, J.L.Chuang, J.Li, D.R.Tomchick, D.T.Chuang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	28.75 / 1.85
*Space group*	P 3₁ 2 1
*Cell size a, b, c (Å), α, β, γ (°)*	144.721, 144.721, 69.151, 90.00, 90.00, 120.00
*R / R_free (%)*	17.2 / 19.9

Other elements in 1ols:

The structure of Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase also contains other interesting chemical elements:

Potassium

(K)

2 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase (pdb code 1ols). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase, PDB code: 1ols:

Manganese binding site 1 out of 1 in 1ols

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Manganese Binding Sites List in 1ols

Manganese binding site 1 out of 1 in the Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase

Mono view

Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Mn503 b:21.2 occ:1.00	O	A:TYR224	2.2	21.2	1.0
	O13	A:TDP601	2.2	17.0	1.0
	OE2	A:GLU193	2.2	18.8	1.0
	O23	A:TDP601	2.2	20.5	1.0
	OD1	A:ASN222	2.2	23.8	1.0
	O	A:HOH2250	2.4	20.6	1.0
	OE1	A:GLU193	2.6	20.4	1.0
	CD	A:GLU193	2.7	20.0	1.0
	CG	A:ASN222	3.2	26.2	1.0
	C	A:TYR224	3.3	22.5	1.0
	P2	A:TDP601	3.3	20.7	1.0
	P1	A:TDP601	3.3	16.8	1.0
	O11	A:TDP601	3.5	19.8	1.0
	ND2	A:ASN222	3.5	25.2	1.0
	N	A:TYR224	4.0	24.3	1.0
	O5G	A:TDP601	4.0	19.0	1.0
	N	A:ALA225	4.1	20.4	1.0
	O21	A:TDP601	4.1	20.9	1.0
	CA	A:ALA225	4.2	18.6	1.0
	N	A:GLU193	4.2	15.2	1.0
	N	A:ASN222	4.2	22.4	1.0
	CA	A:TYR224	4.2	22.6	1.0
	CG	A:GLU193	4.2	17.1	1.0
	CB	A:ASN222	4.5	26.3	1.0
	N	A:GLY194	4.5	16.2	1.0
	O22	A:TDP601	4.5	21.8	1.0
	O12	A:TDP601	4.5	15.1	1.0
	O	A:ARG220	4.5	20.6	1.0
	O	A:HOH2251	4.6	32.5	1.0
	CA	A:ASN222	4.7	24.4	1.0
	CB	A:ALA225	4.7	15.3	1.0
	O	A:HOH2152	4.7	36.4	1.0
	N	A:GLY223	4.8	25.4	1.0
	C	A:ASN222	4.8	26.1	1.0
	CA	A:GLY192	4.8	15.5	1.0
	C	A:GLY192	4.9	15.2	1.0
	CB	A:GLU193	4.9	17.0	1.0
	CA	A:GLU193	5.0	16.6	1.0

Reference:

R.M.Wynn, M.Machius, J.L.Chuang, J.Li, D.R.Tomchick, D.T.Chuang. Roles of HIS291-Alpha and HIS146-Beta' in the Reductive Acylation Reaction Catalyzed By Human Branched-Chain Alpha-Ketoacid Dehydrogenase: Refined Phosphorylation Loop Structure in the Active Site. J.Biol.Chem. V. 278 43402 2003.
ISSN: ISSN 0021-9258
PubMed: 12902323
DOI: 10.1074/JBC.M306204200

Page generated: Sat Oct 5 11:57:52 2024

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