Manganese in PDB 1o0r: Crystal Structure of the Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Complex with Udp-Galactose

All present enzymatic activity of Crystal Structure of the Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Complex with Udp-Galactose:
2.4.1.90;

The structure of Crystal Structure of the Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Complex with Udp-Galactose, PDB code: 1o0r was solved by B.Ramakrishnan, P.V.Balaji, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.53 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.356, 91.775, 142.467, 90.00, 90.00, 90.00
R / Rfree (%)	20.3 / 25.1

The binding sites of Manganese atom in the Crystal Structure of the Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Complex with Udp-Galactose (pdb code 1o0r). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Complex with Udp-Galactose, PDB code: 1o0r:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of the Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Complex with Udp-Galactose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Complex with Udp-Galactose within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn403 b:29.9 occ:1.00 O1A A:GDU404 2.1 28.8 1.0 OD1 A:ASP254 2.2 25.6 1.0 O A:HOH1011 2.2 21.8 1.0 NE2 A:HIS347 2.2 37.7 1.0 O2B A:GDU404 2.2 25.5 1.0 SD A:MET344 2.7 27.6 1.0 CE1 A:HIS347 3.1 37.7 1.0 CG A:ASP254 3.1 27.6 1.0 CD2 A:HIS347 3.3 37.9 1.0 PA A:GDU404 3.4 28.6 1.0 OD2 A:ASP254 3.4 26.4 1.0 PB A:GDU404 3.4 28.1 1.0 O3A A:GDU404 3.6 28.4 1.0 CG A:MET344 3.7 30.7 1.0 CE A:MET344 3.8 30.1 1.0 O3B A:GDU404 3.9 30.5 1.0 O A:HOH1069 3.9 50.0 1.0 O2A A:GDU404 4.1 32.7 1.0 ND1 A:HIS347 4.3 37.8 1.0 O3D A:GDU404 4.3 29.6 1.0 C5D A:GDU404 4.4 31.6 1.0 CG A:HIS347 4.4 39.9 1.0 O5D A:GDU404 4.5 29.1 1.0 CB A:ASP254 4.5 25.4 1.0 OD2 A:ASP252 4.5 31.7 1.0 NH2 A:ARG191 4.6 39.4 1.0 NH1 A:ARG191 4.7 37.3 1.0 O1B A:GDU404 4.7 26.0 1.0 NZ A:LYS279 4.8 43.8 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of the Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Complex with Udp-Galactose


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Complex with Udp-Galactose within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn805 b:31.8 occ:1.00 O B:HOH1002 2.1 22.1 1.0 O1A B:GDU806 2.1 29.9 1.0 OD1 B:ASP254 2.3 28.4 1.0 O2B B:GDU806 2.3 32.2 1.0 NE2 B:HIS347 2.5 39.8 1.0 SD B:MET344 2.7 32.0 1.0 CG B:ASP254 3.1 30.1 1.0 OD2 B:ASP254 3.3 29.1 1.0 CD2 B:HIS347 3.3 40.1 1.0 PA B:GDU806 3.4 33.5 1.0 PB B:GDU806 3.4 31.9 1.0 CE1 B:HIS347 3.5 41.5 1.0 CE B:MET344 3.6 28.7 1.0 O3A B:GDU806 3.6 30.6 1.0 O B:HOH1231 3.6 41.8 1.0 CG B:MET344 3.8 26.3 1.0 O3B B:GDU806 3.8 32.8 1.0 O2A B:GDU806 4.2 35.7 1.0 O B:HOH1176 4.2 42.1 1.0 OD2 B:ASP252 4.3 28.4 1.0 C5D B:GDU806 4.3 36.8 1.0 O3D B:GDU806 4.3 33.1 1.0 O5D B:GDU806 4.4 34.6 1.0 CG B:HIS347 4.5 40.1 1.0 ND1 B:HIS347 4.5 41.1 1.0 CB B:ASP254 4.6 28.8 1.0 NZ B:LYS279 4.6 47.2 1.0 NH2 B:ARG191 4.7 33.0 1.0 NH1 B:ARG191 4.7 32.5 1.0 O1B B:GDU806 4.8 32.4 1.0

B.Ramakrishnan, P.V.Balaji, P.K.Qasba. Crystal Structure of Beta 1,4-Galactosyltransferase Complex with Udp-Gal Reveals An Oligosaccharide Acceptor Binding Site J.Mol.Biol. V. 318 491 2002.
ISSN: ISSN 0022-2836
PubMed: 12051854
DOI: 10.1016/S0022-2836(02)00020-7