Manganese in PDB 1n38: Reovirus Polymerase LAMBDA3 Elongation Complex with One Phosphodiester Bond Formed

The structure of Reovirus Polymerase LAMBDA3 Elongation Complex with One Phosphodiester Bond Formed, PDB code: 1n38 was solved by Y.Tao, D.L.Farsetta, M.L.Nibert, S.C.Harrison, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.67 / 2.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	70.719, 85.207, 248.473, 90.00, 90.00, 90.00
R / Rfree (%)	20.1 / 26.3

The binding sites of Manganese atom in the Reovirus Polymerase LAMBDA3 Elongation Complex with One Phosphodiester Bond Formed (pdb code 1n38). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Reovirus Polymerase LAMBDA3 Elongation Complex with One Phosphodiester Bond Formed, PDB code: 1n38:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Reovirus Polymerase LAMBDA3 Elongation Complex with One Phosphodiester Bond Formed


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Reovirus Polymerase LAMBDA3 Elongation Complex with One Phosphodiester Bond Formed within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1301 b:63.8 occ:1.00 O2B A:U3H1291 2.1 94.2 1.0 OD2 A:ASP734 2.2 35.5 1.0 O A:ILE586 2.4 32.1 1.0 OD1 A:ASP585 2.7 40.5 1.0 CG A:ASP734 3.3 33.6 1.0 C A:ILE586 3.3 30.8 1.0 MN A:MN1302 3.4 39.1 1.0 O1A A:U3H1291 3.4 89.7 1.0 CG A:ASP585 3.5 39.4 1.0 O3G A:U3H1291 3.5 95.3 1.0 PB A:U3H1291 3.6 94.0 1.0 OD2 A:ASP585 3.6 40.0 1.0 O1G A:U3H1291 3.8 94.9 1.0 CA A:SER587 3.8 28.8 1.0 OD1 A:ASP734 3.9 33.3 1.0 N A:SER587 3.9 29.3 1.0 N A:ILE586 4.0 32.1 1.0 O3B A:U3H1291 4.2 94.7 1.0 CB A:ASP734 4.3 30.5 1.0 CA A:ILE586 4.3 31.1 1.0 O3A A:U3H1291 4.3 91.8 1.0 PA A:U3H1291 4.3 90.3 1.0 C5' A:U3H1291 4.3 89.3 1.0 PG A:U3H1291 4.4 95.6 1.0 N A:ALA588 4.5 28.8 1.0 O5' A:U3H1291 4.6 90.1 1.0 C A:SER587 4.6 28.3 1.0 O1B A:U3H1291 4.7 93.5 1.0 CB A:ASP585 4.8 36.0 1.0 CB A:SER587 4.9 28.2 1.0 N A:CYS589 4.9 28.3 1.0 O A:ASP734 4.9 28.0 1.0 C A:ASP585 4.9 33.1 1.0 CB A:CYS589 5.0 30.8 1.0

Manganese binding site 2 out of 2 in the Reovirus Polymerase LAMBDA3 Elongation Complex with One Phosphodiester Bond Formed


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Reovirus Polymerase LAMBDA3 Elongation Complex with One Phosphodiester Bond Formed within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1302 b:39.1 occ:1.00 O1A A:U3H1291 2.3 89.7 1.0 OD1 A:ASP734 2.3 33.3 1.0 OD2 A:ASP585 2.8 40.0 1.0 O5' A:U3H1291 2.9 90.1 1.0 CG A:ASP734 3.0 33.6 1.0 OD2 A:ASP734 3.0 35.5 1.0 OD1 A:ASP735 3.1 39.3 1.0 PA A:U3H1291 3.1 90.3 1.0 MN A:MN1301 3.4 63.8 1.0 C5' A:U3H1291 3.5 89.3 1.0 CG A:ASP585 3.8 39.4 1.0 O B:HOH1543 3.9 30.4 1.0 OD1 A:ASP585 4.1 40.5 1.0 O2A A:U3H1291 4.1 89.8 1.0 O2B A:U3H1291 4.2 94.2 1.0 CG A:ASP735 4.3 36.3 1.0 O3A A:U3H1291 4.4 91.8 1.0 CB A:ASP734 4.4 30.5 1.0 C A:ASP734 4.4 28.6 1.0 O A:ASP734 4.4 28.0 1.0 N A:ASP735 4.6 29.5 1.0 CB A:ASP735 4.6 32.4 1.0 C4' A:U3H1291 4.7 88.9 1.0 CA A:ASP735 4.8 29.2 1.0 PB A:U3H1291 4.8 94.0 1.0 CA A:ASP734 4.9 28.5 1.0 C3' B:C1276 5.0 96.5 1.0

Y.Tao, D.L.Farsetta, M.L.Nibert, S.C.Harrison. Rna Synthesis in A Cage--Structural Studies of Reovirus Polymerase [Lambda] 3 Cell(Cambridge,Mass.) V. 111 733 2002.
ISSN: ISSN 0092-8674
PubMed: 12464184
DOI: 10.1016/S0092-8674(02)01110-8