Manganese in the structure of LAMBDA3 Elongation Complex With Four Phosphodiester Bond Formed (pdb 1n35)

The binding sites of Manganese atom in the structure of LAMBDA3 Elongation Complex With Four Phosphodiester Bond Formed (pdb code 1n35). This binding sites where shown with 5.0 Angstroms radius around Manganese atom. The 1n35 structure was solved by Y.TAO, D.L.FARSETTA, M.L.NIBERT, S.C.HARRISON, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 43.0-2.5 Space group P212121 a (A) 70.883 b (A) 85.013 c (A) 249.294 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 20.7 Rfree (%) 25.9 Manganese Binding Sites: Manganese binding site 1 out of 2 in 1n35 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Manganese in the PDB 1n35. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asp585, A: Ile586, A: Ser587, A: Ala588, A: Cys589, A: Asp734, A: Mn1302, A: Ch11291, conact list: Atom Atom Distance (A) Mn CB A:Asp585 4.91 Mn OD2 A:Asp585 3.68 Mn C A:Asp585 4.97 Mn OD1 A:Asp585 2.70 Mn CG A:Asp585 3.53 Mn O A:Ile586 2.48 Mn N A:Ile586 4.13 Mn C A:Ile586 3.40 Mn CA A:Ile586 4.41 Mn N A:Ser587 4.04 Mn CB A:Ser587 4.92 Mn C A:Ser587 4.72 Mn CA A:Ser587 3.91 Mn N A:Ala588 4.71 Mn CB A:Cys589 4.87 Mn O A:Asp734 4.98 Mn CB A:Asp734 4.40 Mn OD2 A:Asp734 2.19 Mn OD1 A:Asp734 3.90 Mn CG A:Asp734 3.30 Mn MN A:Mn1302 3.35 Mn PG A:Ch11291 3.94 Mn O3B A:Ch11291 4.01 Mn PA A:Ch11291 4.25 Mn O3G A:Ch11291 3.20 Mn O1B A:Ch11291 4.80 Mn PB A:Ch11291 3.57 Mn C5' A:Ch11291 4.84 Mn O5' A:Ch11291 4.92 Mn O2B A:Ch11291 2.28 Mn O3A A:Ch11291 4.35 Mn O1G A:Ch11291 3.14 Mn O1A A:Ch11291 3.09 interactive model: Manganese binding site 2 out of 2 in 1n35 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Manganese in the PDB 1n35. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: G1276, A: Asp585, A: Asp734, A: Asp735, A: Mn1301, A: Ch11291, conact list: Atom Atom Distance (A) Mn O3' B:G1276 4.93 Mn OD2 A:Asp585 2.71 Mn OD1 A:Asp585 3.98 Mn CG A:Asp585 3.69 Mn O A:Asp734 4.19 Mn N A:Asp734 4.92 Mn CB A:Asp734 4.33 Mn OD2 A:Asp734 3.09 Mn C A:Asp734 4.17 Mn OD1 A:Asp734 2.32 Mn CG A:Asp734 2.98 Mn CA A:Asp734 4.69 Mn N A:Asp735 4.33 Mn CB A:Asp735 4.45 Mn OD1 A:Asp735 2.92 Mn CG A:Asp735 4.00 Mn CA A:Asp735 4.58 Mn MN A:Mn1301 3.35 Mn PA A:Ch11291 3.73 Mn O3G A:Ch11291 5.00 Mn O2A A:Ch11291 4.61 Mn C5' A:Ch11291 4.00 Mn O5' A:Ch11291 3.69 Mn O2B A:Ch11291 4.56 Mn O3A A:Ch11291 4.94 Mn O1A A:Ch11291 2.57 interactive model: