Manganese in the structure of Reovirus Polymerase LAMBDA3 Bound to Mrna Cap Analog (pdb 1mwh)

The binding sites of Manganese atom in the structure of Reovirus Polymerase LAMBDA3 Bound to Mrna Cap Analog (pdb code 1mwh). This binding sites where shown with 5.0 Angstroms radius around Manganese atom. The 1mwh structure was solved by Y.TAO, D.L.FARSETTA, M.L.NIBERT, S.C.HARRISON, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 49.9-2.5 Space group P212121 a (A) 70.891 b (A) 84.944 c (A) 249.246 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 20.9 Rfree (%) 24.5 Manganese Binding Sites: Manganese binding site 1 out of 1 in 1mwh Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Manganese in the PDB 1mwh. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asn583, A: Ile584, A: Asp585, A: Asp735, A: Gly736, A: Glu780, A: Tyr781, A: Hoh2349, A: Hoh2382, A: Hoh2423, A: Hoh2433, conact list: Atom Atom Distance (A) Mn CB A:Asn583 4.59 Mn O A:Ile584 4.33 Mn C A:Ile584 4.83 Mn CB A:Asp585 4.32 Mn OD2 A:Asp585 2.74 Mn OD1 A:Asp585 2.24 Mn CG A:Asp585 2.83 Mn CB A:Asp735 3.82 Mn OD2 A:Asp735 2.84 Mn C A:Asp735 4.83 Mn OD1 A:Asp735 1.50 Mn CG A:Asp735 2.46 Mn CA A:Asp735 4.31 Mn O A:Gly736 4.35 Mn N A:Gly736 4.41 Mn O A:Glu780 3.68 Mn OE1 A:Glu780 1.91 Mn CB A:Glu780 4.69 Mn OE2 A:Glu780 3.33 Mn CD A:Glu780 2.95 Mn C A:Glu780 4.33 Mn CG A:Glu780 4.28 Mn N A:Tyr781 4.83 Mn CA A:Tyr781 4.74 Mn O A:Hoh2349 4.01 Mn O A:Hoh2382 2.25 Mn O A:Hoh2423 4.10 Mn O A:Hoh2433 2.27 interactive model: