Manganese in PDB 1mus: Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna

The structure of Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna, PDB code: 1mus was solved by H.M.Holden, J.B.Thoden, M.Steiniger-White, W.S.Reznikoff, S.Lovell, I.Rayment, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.90
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	112.700, 112.700, 235.900, 90.00, 90.00, 120.00
R / Rfree (%)	19 / 24.7

The structure of Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Manganese atom in the Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna (pdb code 1mus). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna, PDB code: 1mus:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn478 b:21.3 occ:1.00 O3' B:DG20 1.9 27.8 1.0 OP1 C:DC1 2.1 21.4 1.0 OE2 A:GLU326 2.1 23.3 1.0 OD2 A:ASP97 2.2 18.6 1.0 O B:HOH21 2.3 17.6 1.0 OE1 A:GLU326 2.5 22.6 1.0 CD A:GLU326 2.6 29.9 1.0 CG A:ASP97 3.2 22.6 1.0 C3' B:DG20 3.3 22.2 1.0 P C:DC1 3.3 40.6 1.0 OD1 A:ASP97 3.5 25.7 1.0 OP3 C:DC1 3.6 42.8 1.0 MN A:MN479 3.8 22.7 1.0 O5' C:DC1 3.8 72.7 1.0 C4' B:DG20 3.9 0.0 1.0 O A:HOH485 4.0 19.8 1.0 C2' B:DG20 4.0 28.2 1.0 CG A:GLU326 4.1 20.8 1.0 O A:THR98 4.1 16.9 1.0 ND1 A:HIS329 4.3 14.2 1.0 CE1 A:HIS329 4.3 7.9 1.0 NH1 A:ARG322 4.3 17.9 1.0 OP1 B:DG20 4.4 21.2 1.0 CB A:ASP97 4.5 11.9 1.0 OP2 C:DC1 4.5 29.1 1.0 OD2 A:ASP188 4.6 23.6 1.0 NH2 A:ARG322 4.6 21.3 1.0 C5' C:DC1 4.7 27.3 1.0 C5' B:DG20 4.8 26.4 1.0 C4' C:DC1 4.9 32.0 1.0 CZ A:ARG322 5.0 20.2 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn479 b:22.7 occ:1.00 OD1 A:ASP97 2.1 25.7 1.0 OP1 C:DC1 2.1 21.4 1.0 OD2 A:ASP188 2.2 23.6 1.0 O A:HOH486 2.2 21.2 1.0 O A:HOH485 2.2 19.8 1.0 OP2 C:DC1 2.4 29.1 1.0 P C:DC1 2.8 40.6 1.0 CG A:ASP188 3.2 22.2 1.0 CG A:ASP97 3.2 22.6 1.0 OD1 A:ASP188 3.4 29.7 1.0 OD2 A:ASP97 3.7 18.6 1.0 MN A:MN478 3.8 21.3 1.0 OP3 C:DC1 3.9 42.8 1.0 O A:HOH500 4.1 16.4 1.0 O5' C:DC1 4.1 72.7 1.0 C5' C:DC1 4.2 27.3 1.0 OE1 A:GLU190 4.3 29.7 1.0 N A:THR98 4.4 21.1 1.0 O A:THR98 4.4 16.9 1.0 CB A:ASP188 4.5 14.6 1.0 CB A:ASP97 4.5 11.9 1.0 O A:HOH810 4.6 55.0 1.0 O C:HOH49 4.6 43.1 1.0 O3' B:DG20 4.8 27.8 1.0 CA A:ASP97 4.8 14.9 1.0 O B:HOH21 4.9 17.6 1.0 NH1 A:ARG322 4.9 17.9 1.0 CB A:GLU190 4.9 23.2 1.0 OE2 A:GLU326 4.9 23.3 1.0

M.Steiniger-White, I.Rayment, W.S.Reznikoff. Structure/Function Insights Into TN5 Transposition. Curr.Opin.Struct.Biol. V. 14 50 2004.
ISSN: ISSN 0959-440X
PubMed: 15102449
DOI: 10.1016/J.SBI.2004.01.008