Manganese in the structure of Crystal Structure of TN5 Transposase Complexed With Resolved Outside End Dna (pdb 1mus)

The binding sites of Manganese atom in the structure of Crystal Structure of TN5 Transposase Complexed With Resolved Outside End Dna (pdb code 1mus). This binding sites where shown with 5.0 Angstroms radius around Manganese atom. The 1mus structure was solved by H.M.HOLDEN, J.B.THODEN, M.STEINIGER-WHITE, W.S.REZNIKOFF, S.LOVELL, I.RAYMENT, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 30.0-1.9 Space group P6522 a (A) 112.700 b (A) 112.700 c (A) 235.900 alpha (°) 90.00 beta (°) 90.00 gamma (°) 120.00 Rfactor (%) 19 Rfree (%) 24.7 Manganese Binding Sites: Manganese binding site 1 out of 2 in 1mus Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Manganese in the PDB 1mus. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Dg20, C: Dc1, A: Asp97, A: Thr98, A: Asp188, A: Arg322, A: Glu326, A: His329, A: Mn479, B: Hoh21, A: Hoh485, conact list: Atom Atom Distance (A) Mn OP1 B:Dg20 4.41 Mn C3' B:Dg20 3.26 Mn O3' B:Dg20 1.90 Mn C5' B:Dg20 4.80 Mn C2' B:Dg20 4.03 Mn C4' B:Dg20 3.92 Mn OP1 C:Dc1 2.07 Mn P C:Dc1 3.28 Mn OP2 C:Dc1 4.48 Mn C5' C:Dc1 4.69 Mn C4' C:Dc1 4.93 Mn O5' C:Dc1 3.82 Mn OP3 C:Dc1 3.60 Mn CB A:Asp97 4.46 Mn OD2 A:Asp97 2.18 Mn OD1 A:Asp97 3.49 Mn CG A:Asp97 3.19 Mn O A:Thr98 4.11 Mn OD2 A:Asp188 4.56 Mn CZ A:Arg322 4.99 Mn NH2 A:Arg322 4.62 Mn NH1 A:Arg322 4.32 Mn OE1 A:Glu326 2.45 Mn OE2 A:Glu326 2.08 Mn CD A:Glu326 2.60 Mn CG A:Glu326 4.11 Mn ND1 A:His329 4.26 Mn CE1 A:His329 4.27 Mn MN A:Mn479 3.80 Mn O B:Hoh21 2.27 Mn O A:Hoh485 3.98 interactive model: Manganese binding site 2 out of 2 in 1mus Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Manganese in the PDB 1mus. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Dg20, C: Dc1, A: Asp97, A: Thr98, A: Asp188, A: Glu190, A: Arg322, A: Glu326, A: Mn478, B: Hoh21, C: Hoh49, A: Hoh485, A: Hoh486, A: Hoh500, A: Hoh810, conact list: Atom Atom Distance (A) Mn O3' B:Dg20 4.80 Mn OP1 C:Dc1 2.15 Mn P C:Dc1 2.82 Mn OP2 C:Dc1 2.38 Mn C5' C:Dc1 4.21 Mn O5' C:Dc1 4.06 Mn OP3 C:Dc1 3.85 Mn CB A:Asp97 4.50 Mn OD2 A:Asp97 3.69 Mn OD1 A:Asp97 2.11 Mn CG A:Asp97 3.19 Mn CA A:Asp97 4.83 Mn O A:Thr98 4.45 Mn N A:Thr98 4.41 Mn CB A:Asp188 4.48 Mn OD2 A:Asp188 2.15 Mn OD1 A:Asp188 3.40 Mn CG A:Asp188 3.15 Mn OE1 A:Glu190 4.28 Mn CB A:Glu190 4.92 Mn NH1 A:Arg322 4.85 Mn OE2 A:Glu326 4.95 Mn MN A:Mn478 3.80 Mn O B:Hoh21 4.85 Mn O C:Hoh49 4.59 Mn O A:Hoh485 2.23 Mn O A:Hoh486 2.21 Mn O A:Hoh500 4.05 Mn O A:Hoh810 4.56 interactive model: