Manganese in PDB 1mm8: Crystal Structure of TN5 Transposase Complexed with Me Dna

The structure of Crystal Structure of TN5 Transposase Complexed with Me Dna, PDB code: 1mm8 was solved by M.Steiniger-White, A.Bhasin, S.Lovell, I.Rayment, W.S.Reznikoff, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	500.00 / 2.80
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	112.500, 112.500, 233.500, 90.00, 90.00, 120.00
R / Rfree (%)	21.7 / 27.1

The binding sites of Manganese atom in the Crystal Structure of TN5 Transposase Complexed with Me Dna (pdb code 1mm8). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of TN5 Transposase Complexed with Me Dna, PDB code: 1mm8:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of TN5 Transposase Complexed with Me Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of TN5 Transposase Complexed with Me Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn500 b:50.5 occ:1.00 O A:HOH714 2.0 32.3 1.0 OD2 A:ASP97 2.1 28.9 1.0 O3' B:DG120 2.4 50.6 1.0 OE1 A:GLU326 2.5 37.4 1.0 OE2 A:GLU326 2.9 38.5 1.0 C3' B:DG120 2.9 47.5 1.0 CD A:GLU326 3.0 37.7 1.0 CG A:ASP97 3.2 28.5 1.0 OD1 A:ASP97 3.6 30.5 1.0 C4' B:DG120 3.6 46.8 1.0 O A:THR98 3.7 22.6 1.0 OP1 B:DG120 3.7 37.8 1.0 O A:HOH749 3.8 52.2 1.0 C5' B:DG120 4.2 42.4 1.0 C2' B:DG120 4.3 49.8 1.0 MN A:MN501 4.4 58.5 0.5 ND1 A:HIS329 4.4 7.5 1.0 CE1 A:HIS329 4.4 6.7 1.0 CB A:ASP97 4.5 26.8 1.0 CG A:GLU326 4.5 35.4 1.0 NH1 A:ARG322 4.6 10.8 1.0 OD2 A:ASP188 4.8 46.7 1.0 O4' B:DG120 4.8 46.1 1.0 C A:THR98 4.9 21.4 1.0 P B:DG120 4.9 39.1 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of TN5 Transposase Complexed with Me Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of TN5 Transposase Complexed with Me Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn501 b:58.5 occ:0.50 OD2 A:ASP188 2.7 46.7 1.0 OD1 A:ASP97 2.7 30.5 1.0 O A:HOH714 2.8 32.3 1.0 O A:HOH692 2.9 50.7 1.0 CG A:ASP188 3.5 44.7 1.0 OD1 A:ASP188 3.7 47.4 1.0 CG A:ASP97 3.8 28.5 1.0 O A:HOH749 3.8 52.2 1.0 CG A:GLU190 4.1 49.7 1.0 O5' C:DC201 4.1 65.3 1.0 OD2 A:ASP97 4.2 28.9 1.0 MN A:MN500 4.4 50.5 1.0 CB A:GLU190 4.5 44.0 1.0 N A:THR98 4.7 25.9 1.0 O A:THR98 4.8 22.6 1.0 CB A:ASP188 5.0 40.1 1.0

M.Steiniger-White, A.Bhasin, S.Lovell, I.Rayment, W.S.Reznikoff. Evidence For "Unseen" Transposase--Dna Contacts J.Mol.Biol. V. 322 971 2002.
ISSN: ISSN 0022-2836
PubMed: 12367522
DOI: 10.1016/S0022-2836(02)00877-X