Manganese in PDB 1mb0: Crystal Structure of the Response Regulator Divk at pH 8.0 in Complex with MN2+

The structure of Crystal Structure of the Response Regulator Divk at pH 8.0 in Complex with MN2+, PDB code: 1mb0 was solved by V.Guillet, N.Ohta, S.Cabantous, A.Newton, J.-P.Samama, Structuralproteomics In Europe (Spine), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.95 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	36.120, 40.760, 66.020, 90.00, 90.00, 90.00
R / Rfree (%)	20.8 / 24.6

The binding sites of Manganese atom in the Crystal Structure of the Response Regulator Divk at pH 8.0 in Complex with MN2+ (pdb code 1mb0). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the Response Regulator Divk at pH 8.0 in Complex with MN2+, PDB code: 1mb0:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of the Response Regulator Divk at pH 8.0 in Complex with MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Response Regulator Divk at pH 8.0 in Complex with MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn201 b:46.4 occ:1.00 OE2 A:GLU9 2.4 24.8 1.0 OD1 A:ASP10 2.4 34.5 1.0 O A:HOH221 2.5 29.4 1.0 OD2 A:ASP53 2.5 32.8 1.0 O A:HOH203 2.8 34.1 1.0 CD A:GLU9 3.2 23.0 1.0 CG A:ASP53 3.2 31.6 1.0 OE1 A:GLU9 3.3 23.6 1.0 OD1 A:ASP53 3.6 31.2 1.0 CG A:ASP10 3.6 33.8 1.0 N A:ASN11 4.0 28.5 1.0 CB A:ASN11 4.1 28.9 1.0 OD1 A:ASN14 4.1 33.5 1.0 N A:ASP10 4.2 26.5 1.0 OD2 A:ASP10 4.3 36.8 1.0 CB A:ASP53 4.3 29.8 1.0 O A:GLN55 4.5 31.3 1.0 CG A:GLU9 4.6 23.4 1.0 CB A:ASP10 4.6 31.5 1.0 CA A:ASP10 4.7 29.4 1.0 CA A:ASN11 4.7 27.2 1.0 C A:ASP10 4.8 28.3 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of the Response Regulator Divk at pH 8.0 in Complex with MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Response Regulator Divk at pH 8.0 in Complex with MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn202 b:54.4 occ:1.00 NE2 A:HIS19 2.6 27.3 1.0 O A:HOH205 2.8 37.9 1.0 OE1 A:GLU23 3.4 31.8 0.5 CD2 A:HIS19 3.5 24.7 1.0 CE1 A:HIS19 3.5 24.7 1.0 O A:HOH231 3.9 38.1 1.0 OG1 A:THR29 4.4 27.9 1.0 NE2 A:GLN31 4.5 30.2 1.0 CD A:GLU23 4.6 30.6 0.5 ND1 A:HIS19 4.7 25.3 1.0 CG A:HIS19 4.7 24.4 1.0

V.Guillet, N.Ohta, S.Cabantous, A.Newton, J.-P.Samama. Crystallographic and Biochemical Studies of Divk Reveal Novel Features of An Essential Response Regulator in Caulobacter Crescentus. J.Biol.Chem. V. 277 42003 2002.
ISSN: ISSN 0021-9258
PubMed: 12176983
DOI: 10.1074/JBC.M204789200