Manganese in PDB 1lqp: Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate

All present enzymatic activity of Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate:
2.5.1.18;

The structure of Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate, PDB code: 1lqp was solved by C.L.Rife, R.E.Pharris, M.E.Newcomer, R.N.Armstrong, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.19
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.071, 64.901, 76.730, 90.00, 90.00, 90.00
R / Rfree (%)	15.6 / 20

The structure of Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate also contains other interesting chemical elements:

Potassium

(K)

2 atoms

The binding sites of Manganese atom in the Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate (pdb code 1lqp). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate, PDB code: 1lqp:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn3004 b:12.2 occ:1.00 O2P A:FCN4002 2.0 13.4 1.0 OE1 A:GLU110 2.1 13.4 1.0 NE2 B:HIS7 2.1 13.0 1.0 NE2 A:HIS64 2.1 12.9 1.0 O A:FCN4002 2.3 13.3 1.0 CD A:GLU110 3.1 13.1 1.0 CE1 B:HIS7 3.1 13.1 1.0 CD2 A:HIS64 3.1 12.5 1.0 CE1 A:HIS64 3.2 12.7 1.0 CD2 B:HIS7 3.2 12.8 1.0 P A:FCN4002 3.2 13.6 1.0 C1 A:FCN4002 3.3 13.8 1.0 OE2 A:GLU110 3.4 13.0 1.0 C2 A:FCN4002 3.4 14.0 1.0 C3 A:FCN4002 3.6 15.6 1.0 O A:HOH9016 4.0 14.9 1.0 CE2 A:TYR100 4.1 13.6 1.0 OG1 B:THR9 4.1 13.0 1.0 O3P A:FCN4002 4.2 14.5 1.0 OH A:TYR100 4.2 13.9 1.0 ND1 B:HIS7 4.3 12.6 1.0 O1P A:FCN4002 4.3 14.4 1.0 ND1 A:HIS64 4.3 12.5 1.0 CG A:HIS64 4.3 12.3 1.0 CG B:HIS7 4.3 13.0 1.0 CG A:GLU110 4.4 12.8 1.0 CB A:ALA66 4.5 13.5 1.0 CZ A:TYR100 4.6 13.3 1.0 CB A:GLU110 4.7 12.5 1.0 CB B:THR9 4.9 11.8 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Fosfomycin Resistance Protein (Fosa) Containing Bound Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn3003 b:13.6 occ:1.00 O2P B:FCN4001 2.0 15.1 1.0 OE1 B:GLU110 2.1 13.9 1.0 NE2 B:HIS64 2.1 13.4 1.0 NE2 A:HIS7 2.2 13.5 1.0 O B:FCN4001 2.3 15.4 1.0 CD B:GLU110 3.1 14.6 1.0 CE1 A:HIS7 3.1 14.5 1.0 CD2 B:HIS64 3.2 12.7 1.0 CE1 B:HIS64 3.2 13.5 1.0 CD2 A:HIS7 3.2 14.6 1.0 P B:FCN4001 3.2 15.2 1.0 C1 B:FCN4001 3.3 15.6 1.0 OE2 B:GLU110 3.4 14.9 1.0 C2 B:FCN4001 3.4 15.7 1.0 C3 B:FCN4001 3.7 17.0 1.0 O B:HOH9014 4.0 15.7 1.0 CE2 B:TYR100 4.1 14.4 1.0 OG1 A:THR9 4.1 14.2 1.0 O3P B:FCN4001 4.2 16.4 1.0 OH B:TYR100 4.2 15.8 1.0 O1P B:FCN4001 4.2 16.7 1.0 ND1 A:HIS7 4.3 14.5 1.0 ND1 B:HIS64 4.3 13.5 1.0 CG B:HIS64 4.3 12.3 1.0 CG A:HIS7 4.3 13.9 1.0 CG B:GLU110 4.4 13.7 1.0 CB B:ALA66 4.4 14.9 1.0 CZ B:TYR100 4.6 14.2 1.0 CB B:GLU110 4.7 14.2 1.0 CB A:THR9 5.0 13.3 1.0

C.L.Rife, R.E.Pharris, M.E.Newcomer, R.N.Armstrong. Crystal Structure of A Genomically Encoded Fosfomycin Resistance Protein (Fosa) at 1.19 A Resolution By Mad Phasing Off the L-III Edge of Tl(+) J.Am.Chem.Soc. V. 124 11001 2002.
ISSN: ISSN 0002-7863
PubMed: 12224946
DOI: 10.1021/JA026879V