Manganese in the structure of Porcine Kidney Fructose-1,6-Bisphosphatase Complexed With An Amp-Site Inhibitor (pdb 1lev)

The binding sites of Manganese atom in the structure of Porcine Kidney Fructose-1,6-Bisphosphatase Complexed With An Amp-Site Inhibitor (pdb code 1lev). This binding sites where shown with 5.0 Angstroms radius around Manganese atom. The 1lev structure was solved by S.W.WRIGHT, A.A.CARLO, D.E.DANLEY, D.L.HAGEMAN, G.A.KARAM, M.N.MANSOUR, L.D.MCCLURE, J.PANDIT, G.K.SCHULTE, J.L.TREADWAY, I.-K.WANG, P.H.BAUER, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 40.3-2.1 Space group C121 a (A) 118.870 b (A) 73.446 c (A) 78.025 alpha (°) 90.00 beta (°) 106.30 gamma (°) 90.00 Rfactor (%) 19.3 Rfree (%) 25.5 Manganese Binding Sites: Manganese binding site 1 out of 4 in 1lev Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Manganese in the PDB 1lev. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Glu97, A: Asp118, A: Asp121, A: Gly122, A: Glu280, A: F6p338, A: Mn341, A: Hoh363, conact list: Atom Atom Distance (A) Mn OE1 A:Glu97 2.52 Mn OE2 A:Glu97 3.78 Mn CD A:Glu97 3.47 Mn CG A:Glu97 4.78 Mn CB A:Asp118 4.30 Mn OD2 A:Asp118 2.44 Mn OD1 A:Asp118 3.42 Mn CG A:Asp118 3.10 Mn CB A:Asp121 4.21 Mn OD1 A:Asp121 3.50 Mn CG A:Asp121 4.38 Mn CA A:Asp121 4.19 Mn N A:Gly122 4.93 Mn OE1 A:Glu280 4.30 Mn OE2 A:Glu280 2.23 Mn CD A:Glu280 3.34 Mn CG A:Glu280 3.97 Mn C1 A:F6p338 4.14 Mn O1 A:F6p338 3.88 Mn MN A:Mn341 3.29 Mn O A:Hoh363 2.84 interactive model: Manganese binding site 2 out of 4 in 1lev Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Manganese in the PDB 1lev. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Glu97, A: Glu98, A: Asp118, A: Pro119, A: Leu120, A: Asp121, A: Mn340, A: Hoh363, A: Hoh368, conact list: Atom Atom Distance (A) Mn OE1 A:Glu97 3.34 Mn CB A:Glu97 4.53 Mn OE2 A:Glu97 2.63 Mn CD A:Glu97 3.17 Mn CG A:Glu97 4.38 Mn OE2 A:Glu98 3.76 Mn CD A:Glu98 4.85 Mn CB A:Asp118 4.67 Mn OD2 A:Asp118 3.31 Mn OD1 A:Asp118 2.42 Mn CG A:Asp118 3.25 Mn CA A:Asp118 4.99 Mn N A:Pro119 4.97 Mn CD A:Pro119 4.83 Mn O A:Leu120 2.86 Mn N A:Leu120 4.20 Mn C A:Leu120 3.47 Mn CA A:Leu120 4.41 Mn N A:Asp121 3.96 Mn CB A:Asp121 4.90 Mn CA A:Asp121 3.98 Mn MN A:Mn340 3.29 Mn O A:Hoh363 3.14 Mn O A:Hoh368 2.88 interactive model: Manganese binding site 3 out of 4 in 1lev Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 3 of Manganese in the PDB 1lev. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: F: Glu97, F: Asp118, F: Asp121, F: Gly122, F: Glu280, F: F6p338, F: Mn343, F: Hoh383, conact list: Atom Atom Distance (A) Mn OE1 F:Glu97 2.36 Mn OE2 F:Glu97 3.93 Mn CD F:Glu97 3.45 Mn CG F:Glu97 4.69 Mn CB F:Asp118 4.37 Mn OD2 F:Asp118 2.24 Mn OD1 F:Asp118 3.70 Mn CG F:Asp118 3.18 Mn CB F:Asp121 3.84 Mn OD2 F:Asp121 4.56 Mn OD1 F:Asp121 2.47 Mn CG F:Asp121 3.47 Mn CA F:Asp121 4.15 Mn N F:Gly122 4.92 Mn OE1 F:Glu280 4.10 Mn OE2 F:Glu280 2.29 Mn CD F:Glu280 3.19 Mn CG F:Glu280 3.77 Mn C1 F:F6p338 4.34 Mn O1 F:F6p338 3.56 Mn MN F:Mn343 3.35 Mn O F:Hoh383 4.88 interactive model: Manganese binding site 4 out of 4 in 1lev Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 4 of Manganese in the PDB 1lev. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: F: Glu97, F: Glu98, F: Asp118, F: Pro119, F: Leu120, F: Asp121, F: Mn342, conact list: Atom Atom Distance (A) Mn OE1 F:Glu97 3.15 Mn CB F:Glu97 4.45 Mn OE2 F:Glu97 2.38 Mn CD F:Glu97 3.08 Mn CG F:Glu97 4.43 Mn OE2 F:Glu98 3.46 Mn CD F:Glu98 4.63 Mn CB F:Asp118 4.59 Mn OD2 F:Asp118 3.17 Mn OD1 F:Asp118 2.33 Mn CG F:Asp118 3.12 Mn CA F:Asp118 4.96 Mn N F:Pro119 4.98 Mn CD F:Pro119 4.82 Mn O F:Leu120 2.79 Mn N F:Leu120 4.23 Mn C F:Leu120 3.45 Mn CA F:Leu120 4.41 Mn N F:Asp121 3.97 Mn CB F:Asp121 4.61 Mn OD1 F:Asp121 4.78 Mn CA F:Asp121 3.91 Mn MN F:Mn342 3.35 interactive model: