Manganese in PDB 1knk: Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis

The structure of Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis, PDB code: 1knk was solved by S.B.Richard, J.L.Ferrer, M.E.Bowman, A.M.Lillo, C.N.Tetzlaff, D.E.Cane, J.P.Noel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.74 / 2.80
Space group	I 21 3
Cell size a, b, c (Å), α, β, γ (°)	145.630, 145.630, 145.630, 90.00, 90.00, 90.00
R / Rfree (%)	23.2 / 24.9

The binding sites of Manganese atom in the Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis (pdb code 1knk). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis, PDB code: 1knk:

Manganese binding site 1 out of 1 in the Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn201 b:98.0 occ:1.00 OD2 A:ASP8 2.3 62.8 1.0 ND1 A:HIS42 2.5 63.5 1.0 CE1 A:HIS10 2.5 71.2 1.0 NE2 A:HIS10 2.7 70.5 1.0 CG A:ASP8 3.0 61.8 1.0 OD1 A:ASP8 3.1 63.3 1.0 CE1 A:HIS42 3.3 63.9 1.0 CG A:HIS42 3.5 61.7 1.0 ND1 A:HIS10 3.6 70.6 1.0 CB A:HIS42 3.8 57.1 1.0 CD2 A:HIS10 3.9 68.9 1.0 CG A:HIS10 4.3 68.6 1.0 NE2 A:HIS42 4.4 64.3 1.0 CB A:ASP8 4.4 58.0 1.0 CD2 A:HIS42 4.5 63.2 1.0 O A:VAL9 4.8 55.9 1.0

S.B.Richard, J.L.Ferrer, M.E.Bowman, A.M.Lillo, C.N.Tetzlaff, D.E.Cane, J.P.Noel. Structure and Mechanism of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase. An Enzyme in the Mevalonate-Independent Isoprenoid Biosynthetic Pathway. J.Biol.Chem. V. 277 8667 2002.
ISSN: ISSN 0021-9258
PubMed: 11786530
DOI: 10.1074/JBC.C100739200