Manganese in PDB 1knj: Co-Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis, Complexed with Cmp/Mecdp/MN2+

The structure of Co-Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis, Complexed with Cmp/Mecdp/MN2+, PDB code: 1knj was solved by S.B.Richard, J.L.Ferrer, M.E.Bowman, A.M.Lillo, C.N.Tetzlaff, D.E.Cane, J.P.Noel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.71 / 2.80
Space group	I 21 3
Cell size a, b, c (Å), α, β, γ (°)	144.254, 144.254, 144.254, 90.00, 90.00, 90.00
R / Rfree (%)	24.1 / 25

The binding sites of Manganese atom in the Co-Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis, Complexed with Cmp/Mecdp/MN2+ (pdb code 1knj). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Co-Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis, Complexed with Cmp/Mecdp/MN2+, PDB code: 1knj:

Manganese binding site 1 out of 1 in the Co-Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis, Complexed with Cmp/Mecdp/MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Co-Crystal Structure of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase (Ispf) From E. Coli Involved in Mevalonate-Independent Isoprenoid Biosynthesis, Complexed with Cmp/Mecdp/MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn401 b:0.1 occ:1.00 OD2 A:ASP8 2.3 65.6 1.0 ND1 A:HIS42 2.3 62.0 1.0 OD1 A:ASP8 2.5 64.0 1.0 NE2 A:HIS10 2.5 66.4 1.0 CG A:ASP8 2.7 62.0 1.0 CD2 A:HIS10 2.9 65.1 1.0 CG A:HIS42 3.3 59.3 1.0 CE1 A:HIS42 3.3 62.6 1.0 CB A:HIS42 3.5 53.3 1.0 CE1 A:HIS10 3.6 65.2 1.0 CB A:ASP8 4.1 58.5 1.0 O A:VAL9 4.1 53.6 1.0 CG A:HIS10 4.1 64.5 1.0 NE2 A:HIS42 4.4 63.0 1.0 ND1 A:HIS10 4.4 64.8 1.0 CD2 A:HIS42 4.5 61.2 1.0 CA A:VAL39 4.5 53.2 1.0 CG1 A:VAL39 4.6 54.5 1.0 N A:VAL39 4.9 55.0 1.0 O A:ASP38 4.9 56.0 1.0 N A:VAL9 4.9 54.8 1.0 C A:VAL9 5.0 55.0 1.0

S.B.Richard, J.L.Ferrer, M.E.Bowman, A.M.Lillo, C.N.Tetzlaff, D.E.Cane, J.P.Noel. Structure and Mechanism of 2-C-Methyl-D-Erythritol 2,4-Cyclodiphosphate Synthase. An Enzyme in the Mevalonate-Independent Isoprenoid Biosynthetic Pathway. J.Biol.Chem. V. 277 8667 2002.
ISSN: ISSN 0021-9258
PubMed: 11786530
DOI: 10.1074/JBC.C100739200