Manganese in PDB 1k4v: 1.53 A Crystal Structure of the Beta-Galactoside-Alpha-1,3- Galactosyltransferase in Complex with Udp

All present enzymatic activity of 1.53 A Crystal Structure of the Beta-Galactoside-Alpha-1,3- Galactosyltransferase in Complex with Udp:
2.4.1.151;

The structure of 1.53 A Crystal Structure of the Beta-Galactoside-Alpha-1,3- Galactosyltransferase in Complex with Udp, PDB code: 1k4v was solved by E.Boix, G.J.Swaminathan, Y.Zhang, R.Natesh, K.Brew, K.R.Acharya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 1.53
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	45.022, 94.145, 94.382, 90.00, 98.92, 90.00
R / Rfree (%)	14 / 19.1

The binding sites of Manganese atom in the 1.53 A Crystal Structure of the Beta-Galactoside-Alpha-1,3- Galactosyltransferase in Complex with Udp (pdb code 1k4v). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the 1.53 A Crystal Structure of the Beta-Galactoside-Alpha-1,3- Galactosyltransferase in Complex with Udp, PDB code: 1k4v:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the 1.53 A Crystal Structure of the Beta-Galactoside-Alpha-1,3- Galactosyltransferase in Complex with Udp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of 1.53 A Crystal Structure of the Beta-Galactoside-Alpha-1,3- Galactosyltransferase in Complex with Udp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn402 b:10.1 occ:1.00 O1B A:UDP401 2.1 11.3 1.0 OD2 A:ASP225 2.1 9.6 1.0 O A:HOH2750 2.2 11.8 1.0 OD1 A:ASP227 2.2 9.6 1.0 O1A A:UDP401 2.2 10.3 1.0 OD2 A:ASP227 2.4 9.9 1.0 CG A:ASP227 2.6 10.8 1.0 CG A:ASP225 3.2 9.7 1.0 PB A:UDP401 3.3 11.0 1.0 PA A:UDP401 3.4 10.9 1.0 O3A A:UDP401 3.5 10.7 1.0 CB A:ASP225 3.6 8.9 1.0 O3B A:UDP401 3.7 11.3 1.0 NZ A:LYS359 3.8 11.0 1.0 O3' A:UDP401 3.8 9.9 1.0 O A:HOH2157 3.9 18.5 1.0 CB A:ASP227 4.1 9.8 1.0 O A:HOH2061 4.2 13.8 1.0 OD1 A:ASP225 4.2 10.6 1.0 O A:HOH2156 4.3 21.7 1.0 C5' A:UDP401 4.3 10.0 1.0 O5' A:UDP401 4.4 10.3 1.0 O A:HOH2027 4.5 11.2 1.0 O A:HOH2009 4.5 10.1 1.0 O2A A:UDP401 4.5 11.4 1.0 O2B A:UDP401 4.5 11.9 1.0 C3' A:UDP401 4.6 8.1 1.0 O A:HOH2021 4.6 11.9 1.0 C4' A:UDP401 4.8 9.7 1.0 O A:ASP227 4.8 11.3 1.0 CB A:ALA282 4.9 10.2 1.0 N A:ASP227 4.9 9.6 1.0 CA A:ASP227 4.9 9.4 1.0

Manganese binding site 2 out of 2 in the 1.53 A Crystal Structure of the Beta-Galactoside-Alpha-1,3- Galactosyltransferase in Complex with Udp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of 1.53 A Crystal Structure of the Beta-Galactoside-Alpha-1,3- Galactosyltransferase in Complex with Udp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn1402 b:10.0 occ:1.00 O1B B:UDP1401 2.1 10.3 1.0 OD2 B:ASP1225 2.1 10.7 1.0 O B:HOH2751 2.2 10.7 1.0 OD1 B:ASP1227 2.2 9.2 1.0 O1A B:UDP1401 2.2 10.8 1.0 OD2 B:ASP1227 2.4 10.1 1.0 CG B:ASP1227 2.6 10.8 1.0 CG B:ASP1225 3.1 9.3 1.0 PB B:UDP1401 3.2 10.8 1.0 PA B:UDP1401 3.4 10.6 1.0 O3A B:UDP1401 3.5 10.6 1.0 CB B:ASP1225 3.6 7.9 1.0 O3B B:UDP1401 3.7 10.8 1.0 NZ B:LYS1359 3.7 11.9 1.0 O3' B:UDP1401 3.8 8.9 1.0 O B:HOH2051 4.1 17.2 1.0 CB B:ASP1227 4.1 10.6 1.0 O B:HOH2071 4.2 12.9 1.0 OD1 B:ASP1225 4.2 9.8 1.0 C5' B:UDP1401 4.3 8.4 1.0 O5' B:UDP1401 4.3 9.8 1.0 O B:HOH2137 4.4 21.6 1.0 O B:HOH2018 4.4 10.1 1.0 O2B B:UDP1401 4.5 10.5 1.0 O2A B:UDP1401 4.5 10.8 1.0 O B:HOH2043 4.5 12.3 1.0 O B:HOH2002 4.6 11.7 1.0 C3' B:UDP1401 4.6 8.9 1.0 CB B:ALA1282 4.8 9.6 1.0 O B:ASP1227 4.8 10.1 1.0 C4' B:UDP1401 4.9 9.3 1.0 N B:ASP1227 4.9 8.0 1.0 CA B:ASP1227 4.9 9.4 1.0 CA B:ASP1225 5.0 8.2 1.0

E.Boix, G.J.Swaminathan, Y.Zhang, R.Natesh, K.Brew, K.R.Acharya. Structure of Udp Complex of Udp-Galactose:Beta-Galactoside-Alpha -1,3-Galactosyltransferase at 1.53-A Resolution Reveals A Conformational Change in the Catalytically Important C Terminus. J.Biol.Chem. V. 276 48608 2001.
ISSN: ISSN 0021-9258
PubMed: 11592969