Manganese in PDB 1jai: H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Manganese

The structure of H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Manganese, PDB code: 1jai was solved by T.Schweins, K.Scheffzek, R.Assheuer, A.Wittinghofer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	40.000, 40.000, 160.400, 90.00, 90.00, 120.00
R / Rfree (%)	20 / 29

The binding sites of Manganese atom in the H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Manganese (pdb code 1jai). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Manganese, PDB code: 1jai:

Manganese binding site 1 out of 1 in the H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Manganese within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn168 b:13.6 occ:1.00 O2B A:GCP167 2.2 7.9 1.0 O2G A:GCP167 2.2 13.8 1.0 OG1 A:THR35 2.3 16.2 1.0 OG A:SER17 2.3 6.9 1.0 O A:HOH170 2.4 13.9 1.0 O A:HOH177 2.4 15.4 1.0 CB A:THR35 3.1 13.3 1.0 CB A:SER17 3.2 6.6 1.0 PG A:GCP167 3.3 9.9 1.0 PB A:GCP167 3.3 8.8 1.0 C3B A:GCP167 3.5 7.8 1.0 N A:SER17 3.8 7.1 1.0 N A:THR35 3.9 10.9 1.0 CA A:SER17 4.1 6.0 1.0 O A:HOH205 4.1 17.4 1.0 CA A:THR35 4.1 12.9 1.0 CG2 A:THR35 4.1 16.3 1.0 O3G A:GCP167 4.2 10.2 1.0 OD2 A:ASP57 4.3 11.8 1.0 O3A A:GCP167 4.3 6.8 1.0 O2A A:GCP167 4.3 9.4 1.0 OD1 A:ASP57 4.3 13.7 1.0 O1B A:GCP167 4.4 6.7 1.0 O1G A:GCP167 4.4 10.6 1.0 O A:THR58 4.5 19.3 1.0 O A:ASP33 4.6 11.0 1.0 CG A:ASP57 4.7 11.7 1.0 CB A:LYS16 4.7 2.6 1.0 PA A:GCP167 4.7 9.7 1.0 C A:PRO34 4.8 12.7 1.0 O1A A:GCP167 4.8 9.6 1.0 CE A:LYS16 4.9 11.3 1.0 C A:LYS16 4.9 8.1 1.0

T.Schweins, K.Scheffzek, R.Assheuer, A.Wittinghofer. The Role of the Metal Ion in the P21RAS Catalysed Gtp-Hydrolysis: MN2+ Versus MG2+. J.Mol.Biol. V. 266 847 1997.
ISSN: ISSN 0022-2836
PubMed: 9102473
DOI: 10.1006/JMBI.1996.0814