Manganese in PDB 1gww: Alpha-,1,3 Galactosyltransferase - Alpha-D-Glucose Complex

All present enzymatic activity of Alpha-,1,3 Galactosyltransferase - Alpha-D-Glucose Complex:
2.4.1.151;

The structure of Alpha-,1,3 Galactosyltransferase - Alpha-D-Glucose Complex, PDB code: 1gww was solved by E.Boix, Y.Zhang, G.J.Swaminathan, K.Brew, K.R.Acharya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.71 / 1.8
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	45.163, 94.370, 94.760, 90.00, 99.40, 90.00
R / Rfree (%)	21 / 23.2

The binding sites of Manganese atom in the Alpha-,1,3 Galactosyltransferase - Alpha-D-Glucose Complex (pdb code 1gww). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Alpha-,1,3 Galactosyltransferase - Alpha-D-Glucose Complex, PDB code: 1gww:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Alpha-,1,3 Galactosyltransferase - Alpha-D-Glucose Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Alpha-,1,3 Galactosyltransferase - Alpha-D-Glucose Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1370 b:28.4 occ:1.00 O1B A:UDP1369 2.1 27.7 1.0 O A:HOH2214 2.2 27.8 1.0 OD2 A:ASP225 2.3 23.2 1.0 OD1 A:ASP227 2.3 29.8 1.0 O1A A:UDP1369 2.3 25.8 1.0 OD2 A:ASP227 2.5 27.9 1.0 CG A:ASP227 2.8 27.6 1.0 CG A:ASP225 3.2 24.2 1.0 PB A:UDP1369 3.3 31.8 1.0 PA A:UDP1369 3.4 29.0 1.0 O3A A:UDP1369 3.4 29.6 1.0 CB A:ASP225 3.5 23.3 1.0 NZ A:LYS359 3.8 26.0 1.0 O3' A:UDP1369 3.8 28.0 1.0 O3B A:UDP1369 3.8 31.0 1.0 O A:HOH2212 4.1 36.2 1.0 O A:HOH2202 4.1 30.3 1.0 O5' A:UDP1369 4.2 29.2 1.0 CB A:ASP227 4.3 26.6 1.0 O A:HOH2108 4.3 36.8 1.0 OD1 A:ASP225 4.3 25.6 1.0 C5' A:UDP1369 4.4 29.0 1.0 O A:HOH2109 4.5 25.4 1.0 O A:HOH2110 4.5 25.6 1.0 O A:HOH2196 4.5 24.6 1.0 O2B A:UDP1369 4.6 30.4 1.0 C3' A:UDP1369 4.6 29.2 1.0 O2A A:UDP1369 4.6 30.1 1.0 CB A:ALA282 4.7 21.1 1.0 C4' A:UDP1369 4.9 30.0 1.0 CA A:ASP225 5.0 21.8 1.0 O A:ASP227 5.0 24.5 1.0

Manganese binding site 2 out of 2 in the Alpha-,1,3 Galactosyltransferase - Alpha-D-Glucose Complex


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Alpha-,1,3 Galactosyltransferase - Alpha-D-Glucose Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn1370 b:26.4 occ:1.00 O1B B:UDP1369 2.1 21.9 1.0 OD2 B:ASP225 2.2 21.4 1.0 O B:HOH2229 2.2 23.4 1.0 O1A B:UDP1369 2.3 22.9 1.0 OD1 B:ASP227 2.3 27.1 1.0 OD2 B:ASP227 2.5 22.0 1.0 CG B:ASP227 2.8 25.8 1.0 CG B:ASP225 3.2 22.6 1.0 PB B:UDP1369 3.3 25.6 1.0 PA B:UDP1369 3.4 24.9 1.0 O3A B:UDP1369 3.4 23.5 1.0 CB B:ASP225 3.5 19.6 1.0 O3B B:UDP1369 3.7 24.5 1.0 NZ B:LYS359 3.7 22.8 1.0 O3' B:UDP1369 3.8 24.1 1.0 O B:HOH2226 4.1 29.5 1.0 O B:HOH2213 4.1 29.4 1.0 O5' B:UDP1369 4.2 25.1 1.0 OD1 B:ASP225 4.3 25.1 1.0 CB B:ASP227 4.3 24.8 1.0 O B:HOH2230 4.4 36.4 1.0 C5' B:UDP1369 4.4 23.8 1.0 O B:HOH2120 4.5 24.4 1.0 O B:HOH2121 4.5 25.5 1.0 O2B B:UDP1369 4.5 25.4 1.0 C3' B:UDP1369 4.6 24.3 1.0 O2A B:UDP1369 4.6 25.1 1.0 O B:HOH2209 4.6 23.2 1.0 CB B:ALA282 4.7 19.8 1.0 O B:ASP227 4.9 21.5 1.0 C4' B:UDP1369 4.9 25.0 1.0 CA B:ASP225 4.9 21.2 1.0 N B:ASP227 5.0 22.5 1.0

E.Boix, Y.Zhang, G.J.Swaminathan, K.Brew, K.R.Acharya. Structural Basis of Ordered Binding of Donor and Acceptor Substrates to the Retaining Glycosyltransferase, Alpha -1,3 Galactosyltransferase J.Biol.Chem. V. 277 28310 2002.
ISSN: ISSN 0021-9258
PubMed: 12011052
DOI: 10.1074/JBC.M202631200