Manganese in PDB 1g5b: Bacteriophage Lambda Ser/Thr Protein Phosphatase

The structure of Bacteriophage Lambda Ser/Thr Protein Phosphatase, PDB code: 1g5b was solved by W.C.Voegtli, D.J.White, N.J.Reiter, F.Rusnak, A.C.Rosenzweig, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.00 / 2.15
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	160.400, 177.300, 79.100, 90.00, 90.00, 90.00
R / Rfree (%)	20 / 22.6

The structure of Bacteriophage Lambda Ser/Thr Protein Phosphatase also contains other interesting chemical elements:

Mercury

(Hg)

2 atoms

The binding sites of Manganese atom in the Bacteriophage Lambda Ser/Thr Protein Phosphatase (pdb code 1g5b). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 6 binding sites of Manganese where determined in the Bacteriophage Lambda Ser/Thr Protein Phosphatase, PDB code: 1g5b:
Jump to Manganese binding site number: 1; 2; 3; 4; 5; 6;

Manganese binding site 1 out of 6 in the Bacteriophage Lambda Ser/Thr Protein Phosphatase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Bacteriophage Lambda Ser/Thr Protein Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1005 b:31.6 occ:1.00 OD1 A:ASN75 2.1 26.4 1.0 NE2 A:HIS139 2.2 33.7 1.0 ND1 A:HIS186 2.2 29.9 1.0 O A:HOH3056 2.3 34.6 1.0 OD2 A:ASP49 2.3 26.9 1.0 O3 A:SO43001 2.4 46.7 1.0 CE1 A:HIS186 3.0 26.7 1.0 CE1 A:HIS139 3.1 31.3 1.0 CG A:ASP49 3.2 26.5 1.0 CG A:ASN75 3.2 27.3 1.0 CD2 A:HIS139 3.2 36.4 1.0 CG A:HIS186 3.4 29.6 1.0 OD1 A:ASP49 3.4 29.7 1.0 MN A:MN1006 3.5 38.0 1.0 S A:SO43001 3.6 48.2 1.0 CA A:HIS186 3.7 36.6 1.0 ND2 A:ASN75 3.7 29.8 1.0 O1 A:SO43001 3.8 42.5 1.0 CB A:HIS186 3.8 30.3 1.0 OD2 A:ASP20 4.0 34.1 1.0 CD2 A:HIS76 4.0 25.2 1.0 NE2 A:HIS186 4.2 33.7 1.0 ND1 A:HIS139 4.2 34.0 1.0 O A:HIS186 4.3 38.7 1.0 CG A:HIS139 4.3 38.2 1.0 CD2 A:HIS186 4.4 29.8 1.0 O2 A:SO43001 4.4 46.3 1.0 CB A:ASP49 4.5 24.4 1.0 O A:HOH3047 4.5 38.0 1.0 N A:ASN75 4.5 30.7 1.0 CB A:ASN75 4.5 27.0 1.0 C A:HIS186 4.5 37.9 1.0 O A:ILE159 4.6 36.7 1.0 NE2 A:HIS76 4.7 29.0 1.0 O4 A:SO43001 4.7 46.9 1.0 N A:HIS186 4.7 33.6 1.0

Manganese binding site 2 out of 6 in the Bacteriophage Lambda Ser/Thr Protein Phosphatase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Bacteriophage Lambda Ser/Thr Protein Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1006 b:38.0 occ:1.00 O A:HOH3056 2.1 34.6 1.0 NE2 A:HIS22 2.2 27.5 1.0 OD2 A:ASP49 2.2 26.9 1.0 O A:HOH3055 2.3 36.2 1.0 OD2 A:ASP20 2.4 34.1 1.0 O A:HOH3047 2.6 38.0 1.0 CE1 A:HIS22 3.1 29.1 1.0 CG A:ASP49 3.2 26.5 1.0 CD2 A:HIS22 3.3 28.0 1.0 CG A:ASP20 3.3 35.1 1.0 MN A:MN1005 3.5 31.6 1.0 CB A:ASP49 3.6 24.4 1.0 CB A:ASP20 3.7 30.9 1.0 O3 A:SO43001 4.0 46.7 1.0 OD2 A:ASP202 4.2 33.3 1.0 ND1 A:HIS22 4.2 27.2 1.0 O1 A:SO43001 4.3 42.5 1.0 O A:HIS186 4.3 38.7 1.0 OD1 A:ASP49 4.4 29.7 1.0 CG A:HIS22 4.4 29.2 1.0 CE1 A:HIS139 4.4 31.3 1.0 CD2 A:HIS76 4.4 25.2 1.0 OD1 A:ASP20 4.5 34.6 1.0 O A:HOH3021 4.5 40.2 1.0 CA A:HIS186 4.5 36.6 1.0 NE2 A:HIS139 4.6 33.7 1.0 NE2 A:HIS76 4.6 29.0 1.0 S A:SO43001 4.6 48.2 1.0 C A:HIS186 4.8 37.9 1.0 O4 A:SO43001 4.9 46.9 1.0 N A:HIS186 4.9 33.6 1.0

Manganese binding site 3 out of 6 in the Bacteriophage Lambda Ser/Thr Protein Phosphatase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Bacteriophage Lambda Ser/Thr Protein Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn1003 b:27.4 occ:1.00 OD2 B:ASP20 2.2 26.5 1.0 OD2 B:ASP49 2.2 32.4 1.0 NE2 B:HIS22 2.2 21.4 1.0 O B:HOH3011 2.2 26.0 1.0 O B:HOH3012 2.3 29.5 1.0 O B:HOH3088 2.4 42.7 1.0 CE1 B:HIS22 3.2 23.1 1.0 CG B:ASP20 3.2 25.0 1.0 CD2 B:HIS22 3.2 21.5 1.0 CG B:ASP49 3.2 30.8 1.0 MN B:MN1004 3.5 26.9 1.0 CB B:ASP49 3.6 25.9 1.0 CB B:ASP20 3.7 24.7 1.0 O B:HOH3074 3.8 46.3 1.0 O2 B:SO43002 3.9 35.4 1.0 OD2 B:ASP202 4.2 29.3 1.0 O B:HIS186 4.2 25.3 1.0 O4 B:SO43002 4.2 40.0 1.0 OD1 B:ASP20 4.3 22.8 1.0 ND1 B:HIS22 4.3 23.1 1.0 OD1 B:ASP49 4.4 30.6 1.0 CG B:HIS22 4.4 25.4 1.0 CE1 B:HIS139 4.4 27.1 1.0 CD2 B:HIS76 4.4 25.2 1.0 CA B:HIS186 4.4 26.3 1.0 O B:HOH3008 4.5 29.1 1.0 NE2 B:HIS139 4.6 25.5 1.0 S B:SO43002 4.6 38.7 1.0 NE2 B:HIS76 4.7 28.3 1.0 C B:HIS186 4.7 27.0 1.0 ND1 B:HIS186 4.9 25.3 1.0 N B:HIS186 4.9 25.8 1.0

Manganese binding site 4 out of 6 in the Bacteriophage Lambda Ser/Thr Protein Phosphatase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Bacteriophage Lambda Ser/Thr Protein Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn1004 b:26.9 occ:1.00 OD1 B:ASN75 2.2 25.8 1.0 NE2 B:HIS139 2.2 25.5 1.0 ND1 B:HIS186 2.2 25.3 1.0 O2 B:SO43002 2.3 35.4 1.0 O B:HOH3011 2.4 26.0 1.0 OD2 B:ASP49 2.4 32.4 1.0 CE1 B:HIS186 3.0 23.8 1.0 CE1 B:HIS139 3.1 27.1 1.0 CG B:ASN75 3.2 27.9 1.0 CG B:ASP49 3.2 30.8 1.0 CD2 B:HIS139 3.3 25.8 1.0 CG B:HIS186 3.4 26.6 1.0 OD1 B:ASP49 3.4 30.6 1.0 MN B:MN1003 3.5 27.4 1.0 ND2 B:ASN75 3.6 24.3 1.0 S B:SO43002 3.6 38.7 1.0 CA B:HIS186 3.8 26.3 1.0 CB B:HIS186 3.8 25.4 1.0 O4 B:SO43002 3.9 40.0 1.0 OD2 B:ASP20 3.9 26.5 1.0 O B:HOH3088 4.1 42.7 1.0 CD2 B:HIS76 4.1 25.2 1.0 NE2 B:HIS186 4.2 30.0 1.0 O B:HIS186 4.3 25.3 1.0 ND1 B:HIS139 4.3 26.4 1.0 O3 B:SO43002 4.3 41.3 1.0 CG B:HIS139 4.4 29.5 1.0 CD2 B:HIS186 4.4 28.3 1.0 CB B:ASN75 4.5 25.3 1.0 CB B:ASP49 4.5 25.9 1.0 O B:ILE159 4.5 31.4 1.0 C B:HIS186 4.5 27.0 1.0 N B:ASN75 4.6 25.4 1.0 O1 B:SO43002 4.7 41.6 1.0 NE2 B:HIS76 4.7 28.3 1.0 N B:HIS186 4.8 25.8 1.0

Manganese binding site 5 out of 6 in the Bacteriophage Lambda Ser/Thr Protein Phosphatase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 5 of Bacteriophage Lambda Ser/Thr Protein Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn1001 b:28.6 occ:1.00 O1 C:SO43003 2.1 33.9 1.0 NE2 C:HIS22 2.2 22.2 1.0 OD2 C:ASP20 2.2 29.6 1.0 O C:HOH3082 2.2 23.8 1.0 OD2 C:ASP49 2.4 26.1 1.0 O3 C:SO43003 2.6 31.6 1.0 S C:SO43003 2.9 34.9 1.0 CE1 C:HIS22 3.1 25.0 1.0 CD2 C:HIS22 3.2 22.6 1.0 CG C:ASP20 3.2 33.5 1.0 CG C:ASP49 3.3 26.3 1.0 MN C:MN1002 3.4 26.6 1.0 CB C:ASP49 3.6 24.7 1.0 CB C:ASP20 3.6 29.8 1.0 O2 C:SO43003 3.8 31.4 1.0 O4 C:SO43003 4.0 25.8 1.0 O C:HOH3019 4.1 32.2 1.0 OD2 C:ASP202 4.1 29.7 1.0 ND1 C:HIS22 4.2 26.2 1.0 CG C:HIS22 4.3 22.1 1.0 OD1 C:ASP20 4.3 31.5 1.0 CE1 C:HIS139 4.3 25.2 1.0 O C:HIS186 4.4 37.6 1.0 CA C:HIS186 4.5 29.7 1.0 OD1 C:ASP49 4.5 28.4 1.0 O C:HOH3020 4.6 35.7 1.0 NE2 C:HIS139 4.6 27.7 1.0 CD2 C:HIS76 4.6 26.2 1.0 NE C:ARG53 4.6 33.3 1.0 N C:HIS186 4.8 31.3 1.0 C C:HIS186 4.8 33.4 1.0 NE2 C:HIS76 5.0 30.5 1.0 CA C:ASP20 5.0 29.7 1.0

Manganese binding site 6 out of 6 in the Bacteriophage Lambda Ser/Thr Protein Phosphatase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 6 of Bacteriophage Lambda Ser/Thr Protein Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn1002 b:26.6 occ:1.00 OD1 C:ASN75 2.1 30.6 1.0 NE2 C:HIS139 2.2 27.7 1.0 OD2 C:ASP49 2.2 26.1 1.0 ND1 C:HIS186 2.3 28.2 1.0 O2 C:SO43003 2.3 31.4 1.0 O1 C:SO43003 2.4 33.9 1.0 S C:SO43003 2.9 34.9 1.0 CE1 C:HIS139 3.0 25.2 1.0 CE1 C:HIS186 3.1 29.5 1.0 CG C:ASP49 3.1 26.3 1.0 CG C:ASN75 3.2 31.8 1.0 CD2 C:HIS139 3.3 28.9 1.0 CG C:HIS186 3.4 30.5 1.0 OD1 C:ASP49 3.4 28.4 1.0 MN C:MN1001 3.4 28.6 1.0 ND2 C:ASN75 3.6 26.2 1.0 O3 C:SO43003 3.8 31.6 1.0 CA C:HIS186 3.8 29.7 1.0 CB C:HIS186 3.9 29.6 1.0 OD2 C:ASP20 3.9 29.6 1.0 CD2 C:HIS76 4.0 26.2 1.0 O4 C:SO43003 4.1 25.8 1.0 ND1 C:HIS139 4.2 27.8 1.0 NE2 C:HIS186 4.3 28.4 1.0 CG C:HIS139 4.4 29.8 1.0 CB C:ASP49 4.4 24.7 1.0 O C:HIS186 4.4 37.6 1.0 CD2 C:HIS186 4.5 28.2 1.0 CB C:ASN75 4.5 29.1 1.0 O C:ILE159 4.5 31.0 1.0 N C:ASN75 4.6 28.2 1.0 C C:HIS186 4.6 33.4 1.0 N C:HIS186 4.7 31.3 1.0 NE2 C:HIS76 4.7 30.5 1.0

W.C.Voegtli, D.J.White, N.J.Reiter, F.Rusnak, A.C.Rosenzweig. Structure of the Bacteriophage Lambda Ser/Thr Protein Phosphatase with Sulfate Ion Bound in Two Coordination Modes. Biochemistry V. 39 15365 2000.
ISSN: ISSN 0006-2960
PubMed: 11112522
DOI: 10.1021/BI0021030