Manganese in PDB 1g15: Co-Crystal of E. Coli Rnase Hi with Two MN2+ Ions Bound in the the Active Site

All present enzymatic activity of Co-Crystal of E. Coli Rnase Hi with Two MN2+ Ions Bound in the the Active Site:
3.1.26.4;

The structure of Co-Crystal of E. Coli Rnase Hi with Two MN2+ Ions Bound in the the Active Site, PDB code: 1g15 was solved by E.R.Goedken, S.Marqusee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.90
Space group	P 63
Cell size a, b, c (Å), α, β, γ (°)	71.653, 71.653, 48.127, 90.00, 90.00, 120.00
R / Rfree (%)	22.3 / 26.1

The binding sites of Manganese atom in the Co-Crystal of E. Coli Rnase Hi with Two MN2+ Ions Bound in the the Active Site (pdb code 1g15). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Co-Crystal of E. Coli Rnase Hi with Two MN2+ Ions Bound in the the Active Site, PDB code: 1g15:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Co-Crystal of E. Coli Rnase Hi with Two MN2+ Ions Bound in the the Active Site


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Co-Crystal of E. Coli Rnase Hi with Two MN2+ Ions Bound in the the Active Site within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn201 b:17.0 occ:1.00 O A:HOH239 2.1 17.3 1.0 OE2 A:GLU48 2.1 21.8 1.0 OD2 A:ASP10 2.2 14.1 1.0 O A:HOH204 2.2 13.7 1.0 OD1 A:ASP70 2.3 15.5 1.0 O A:HOH203 2.9 16.1 1.0 CD A:GLU48 3.2 20.6 1.0 CG A:ASP70 3.3 17.6 1.0 CG A:ASP10 3.3 12.4 1.0 OD2 A:ASP70 3.5 19.1 1.0 OE1 A:GLU48 3.6 21.8 1.0 OD1 A:ASP10 3.7 12.4 1.0 MN A:MN202 4.0 12.7 1.0 O A:GLY11 4.2 16.1 1.0 O A:HOH241 4.2 10.2 1.0 N A:GLY11 4.3 13.2 1.0 O A:HOH247 4.4 42.6 1.0 N A:SER71 4.4 13.9 1.0 ND2 A:ASN44 4.5 22.4 1.0 OD1 A:ASN44 4.5 24.6 1.0 C A:ASP70 4.5 14.1 1.0 CG A:GLU48 4.5 19.2 1.0 CB A:ASP10 4.5 14.3 1.0 CA A:SER71 4.6 17.3 1.0 CA A:ASP10 4.7 13.0 1.0 CB A:ASP70 4.7 16.8 1.0 O A:HOH243 4.7 37.1 1.0 O A:ASP70 4.7 14.0 1.0 O A:HOH242 4.8 21.7 1.0 CB A:SER71 4.8 16.9 1.0 O A:HOH240 4.9 16.5 1.0 CG A:ASN44 5.0 19.5 1.0

Manganese binding site 2 out of 2 in the Co-Crystal of E. Coli Rnase Hi with Two MN2+ Ions Bound in the the Active Site


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Co-Crystal of E. Coli Rnase Hi with Two MN2+ Ions Bound in the the Active Site within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn202 b:12.7 occ:1.00 OD1 A:ASP134 2.1 12.6 1.0 O A:HOH240 2.2 16.5 1.0 O A:HOH241 2.2 10.2 1.0 OD1 A:ASP10 2.2 12.4 1.0 O A:HOH204 2.4 13.7 1.0 CG A:ASP134 3.2 12.0 1.0 CG A:ASP10 3.2 12.4 1.0 OD2 A:ASP10 3.5 14.1 1.0 CB A:ASP134 3.8 12.3 1.0 O A:HOH239 3.9 17.3 1.0 MN A:MN201 4.0 17.0 1.0 OD2 A:ASP70 4.1 19.1 1.0 O A:GLY11 4.2 16.1 1.0 OD2 A:ASP134 4.2 13.4 1.0 O A:HOH219 4.2 24.3 1.0 CA A:ASP134 4.3 13.6 1.0 NE A:ARG138 4.3 11.2 1.0 NH2 A:ARG138 4.4 10.2 1.0 CB A:ASP10 4.6 14.3 1.0 CZ A:ARG138 4.8 13.8 1.0 O A:HOH220 4.9 36.1 1.0 OD1 A:ASP70 4.9 15.5 1.0 CG A:ASP70 4.9 17.6 1.0

E.R.Goedken, S.Marqusee. Co-Crystal of Escherichia Coli Rnase Hi with MN2+ Ions Reveals Two Divalent Metals Bound in the Active Site. J.Biol.Chem. V. 276 7266 2001.
ISSN: ISSN 0021-9258
PubMed: 11083878
DOI: 10.1074/JBC.M009626200