Manganese in the structure of Co-Crystal of E. Coli Rnase Hi With Two MN2+ Ions Bound in The the Active Site (pdb 1g15)

The binding sites of Manganese atom in the structure of Co-Crystal of E. Coli Rnase Hi With Two MN2+ Ions Bound in The the Active Site (pdb code 1g15). This binding sites where shown with 5.0 Angstroms radius around Manganese atom. The 1g15 structure was solved by E.R.GOEDKEN, S.MARQUSEE, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 20.0-1.9 Space group P63 a (A) 71.653 b (A) 71.653 c (A) 48.127 alpha (°) 90.00 beta (°) 90.00 gamma (°) 120.00 Rfactor (%) 22.3 Rfree (%) 26.1 Manganese Binding Sites: Manganese binding site 1 out of 2 in 1g15 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Manganese in the PDB 1g15. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asp10, A: Gly11, A: Asn44, A: Glu48, A: Asp70, A: Ser71, A: Mn202, A: Hoh203, A: Hoh204, A: Hoh239, A: Hoh240, A: Hoh241, A: Hoh242, A: Hoh243, A: Hoh247, conact list: Atom Atom Distance (A) Mn CB A:Asp10 4.54 Mn OD2 A:Asp10 2.16 Mn OD1 A:Asp10 3.74 Mn CG A:Asp10 3.29 Mn CA A:Asp10 4.65 Mn O A:Gly11 4.25 Mn N A:Gly11 4.28 Mn ND2 A:Asn44 4.48 Mn OD1 A:Asn44 4.51 Mn CG A:Asn44 4.96 Mn OE1 A:Glu48 3.63 Mn OE2 A:Glu48 2.15 Mn CD A:Glu48 3.23 Mn CG A:Glu48 4.52 Mn O A:Asp70 4.74 Mn CB A:Asp70 4.67 Mn OD2 A:Asp70 3.49 Mn C A:Asp70 4.51 Mn OD1 A:Asp70 2.34 Mn CG A:Asp70 3.27 Mn N A:Ser71 4.43 Mn CB A:Ser71 4.85 Mn CA A:Ser71 4.58 Mn MN A:Mn202 3.95 Mn O A:Hoh203 2.85 Mn O A:Hoh204 2.19 Mn O A:Hoh239 2.06 Mn O A:Hoh240 4.91 Mn O A:Hoh241 4.25 Mn O A:Hoh242 4.80 Mn O A:Hoh243 4.74 Mn O A:Hoh247 4.36 interactive model: Manganese binding site 2 out of 2 in 1g15 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Manganese in the PDB 1g15. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asp10, A: Gly11, A: Asp70, A: Asp134, A: Arg138, A: Mn201, A: Hoh204, A: Hoh219, A: Hoh220, A: Hoh239, A: Hoh240, A: Hoh241, conact list: Atom Atom Distance (A) Mn CB A:Asp10 4.58 Mn OD2 A:Asp10 3.54 Mn OD1 A:Asp10 2.22 Mn CG A:Asp10 3.22 Mn O A:Gly11 4.22 Mn OD2 A:Asp70 4.08 Mn OD1 A:Asp70 4.90 Mn CG A:Asp70 4.90 Mn CB A:Asp134 3.77 Mn OD2 A:Asp134 4.22 Mn OD1 A:Asp134 2.15 Mn CG A:Asp134 3.20 Mn CA A:Asp134 4.29 Mn CZ A:Arg138 4.85 Mn NE A:Arg138 4.32 Mn NH2 A:Arg138 4.37 Mn MN A:Mn201 3.95 Mn O A:Hoh204 2.38 Mn O A:Hoh219 4.23 Mn O A:Hoh220 4.86 Mn O A:Hoh239 3.93 Mn O A:Hoh240 2.18 Mn O A:Hoh241 2.21 interactive model: