Manganese in PDB 1fui: L-Fucose Isomerase From Escherichia Coli

All present enzymatic activity of L-Fucose Isomerase From Escherichia Coli:
5.3.1.3;

The structure of L-Fucose Isomerase From Escherichia Coli, PDB code: 1fui was solved by J.E.Seemann, G.E.Schulz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	127.300, 128.300, 239.400, 90.00, 90.00, 90.00
R / Rfree (%)	16.2 / 20.9

The binding sites of Manganese atom in the L-Fucose Isomerase From Escherichia Coli (pdb code 1fui). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 5 binding sites of Manganese where determined in the L-Fucose Isomerase From Escherichia Coli, PDB code: 1fui:
Jump to Manganese binding site number: 1; 2; 3; 4; 5;

Manganese binding site 1 out of 5 in the L-Fucose Isomerase From Escherichia Coli


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of L-Fucose Isomerase From Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn592 b:39.2 occ:1.00 O A:HOH648 2.2 54.4 1.0 O1 A:FOC594 2.3 56.2 1.0 NE2 A:HIS528 2.4 20.4 1.0 OE1 A:GLU337 2.5 28.1 1.0 O2 A:FOC594 2.5 82.3 1.0 OD2 A:ASP361 2.7 51.8 1.0 OD1 A:ASP361 2.7 51.0 1.0 OE2 A:GLU337 3.1 29.6 1.0 CG A:ASP361 3.1 33.4 1.0 CD A:GLU337 3.1 30.3 1.0 C2 A:FOC594 3.2 73.6 1.0 C1 A:FOC594 3.2 69.0 1.0 CE1 A:HIS528 3.2 6.9 1.0 CD2 A:HIS528 3.4 20.3 1.0 OG A:SER393 3.8 44.4 1.0 OD1 A:ASN392 4.2 28.5 1.0 ND1 A:HIS528 4.3 18.3 1.0 ND2 A:ASN527 4.3 26.1 1.0 CG A:HIS528 4.4 16.1 1.0 CG A:GLU337 4.6 21.0 1.0 CB A:ASP361 4.6 26.4 1.0 CE1 A:PHE359 4.6 20.3 1.0 CB A:SER393 4.6 35.8 1.0 C3 A:FOC594 4.6 76.0 1.0 OG1 A:THR336 4.9 23.5 1.0

Manganese binding site 2 out of 5 in the L-Fucose Isomerase From Escherichia Coli


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of L-Fucose Isomerase From Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn592 b:41.9 occ:1.00 O B:HOH650 2.2 55.1 1.0 O1 B:FOC593 2.3 59.7 1.0 O2 B:FOC593 2.3 84.2 1.0 NE2 B:HIS528 2.5 32.4 1.0 OD1 B:ASP361 2.6 45.4 1.0 OE1 B:GLU337 2.6 32.0 1.0 OD2 B:ASP361 2.6 43.4 1.0 C2 B:FOC593 3.0 76.9 1.0 CG B:ASP361 3.0 34.5 1.0 OE2 B:GLU337 3.1 32.8 1.0 C1 B:FOC593 3.1 73.7 1.0 CD B:GLU337 3.2 30.3 1.0 CE1 B:HIS528 3.3 28.1 1.0 CD2 B:HIS528 3.5 32.1 1.0 OG B:SER393 3.7 39.6 1.0 OD1 B:ASN392 4.3 37.3 1.0 C3 B:FOC593 4.4 79.8 1.0 ND1 B:HIS528 4.4 34.2 1.0 ND2 B:ASN527 4.5 34.0 1.0 CB B:ASP361 4.5 25.2 1.0 CB B:SER393 4.5 36.6 1.0 CG B:HIS528 4.6 33.3 1.0 CG B:GLU337 4.7 25.6 1.0 O3 B:FOC593 4.8 83.0 1.0 CE1 B:PHE359 4.8 18.6 1.0 O4 B:FOC593 5.0 86.2 1.0 OG1 B:THR336 5.0 25.3 1.0 N B:SER393 5.0 38.3 1.0

Manganese binding site 3 out of 5 in the L-Fucose Isomerase From Escherichia Coli


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of L-Fucose Isomerase From Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn592 b:43.5 occ:1.00 O1 C:FOC593 2.3 67.6 1.0 O C:HOH656 2.3 61.6 1.0 O2 C:FOC593 2.4 83.8 1.0 NE2 C:HIS528 2.5 32.1 1.0 OD2 C:ASP361 2.6 49.7 1.0 OE1 C:GLU337 2.6 31.3 1.0 OD1 C:ASP361 2.7 46.2 1.0 CG C:ASP361 3.0 36.0 1.0 C2 C:FOC593 3.1 75.0 1.0 OE2 C:GLU337 3.2 25.0 1.0 C1 C:FOC593 3.2 73.5 1.0 CD C:GLU337 3.2 25.2 1.0 CE1 C:HIS528 3.3 29.8 1.0 CD2 C:HIS528 3.4 33.3 1.0 OG C:SER393 3.8 36.8 1.0 OD1 C:ASN392 4.3 41.9 1.0 ND2 C:ASN527 4.4 23.6 1.0 ND1 C:HIS528 4.4 28.9 1.0 CG C:HIS528 4.5 28.6 1.0 CB C:ASP361 4.5 28.4 1.0 C3 C:FOC593 4.6 75.8 1.0 CB C:SER393 4.6 41.2 1.0 CE1 C:PHE359 4.6 22.5 1.0 CG C:GLU337 4.6 25.1 1.0 O3 C:FOC593 4.9 75.6 1.0 N C:SER393 5.0 45.8 1.0

Manganese binding site 4 out of 5 in the L-Fucose Isomerase From Escherichia Coli


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of L-Fucose Isomerase From Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn592 b:49.9 occ:1.00 O D:HOH659 2.2 58.3 1.0 O1 D:FOC594 2.4 68.0 1.0 O2 D:FOC594 2.4 83.1 1.0 OD1 D:ASP361 2.4 56.7 1.0 NE2 D:HIS528 2.5 29.0 1.0 OD2 D:ASP361 2.6 46.1 1.0 OE1 D:GLU337 2.8 47.7 1.0 CG D:ASP361 2.9 35.0 1.0 C2 D:FOC594 3.1 75.5 1.0 C1 D:FOC594 3.2 69.9 1.0 OE2 D:GLU337 3.3 46.3 1.0 CD D:GLU337 3.4 38.3 1.0 CE1 D:HIS528 3.4 28.3 1.0 CD2 D:HIS528 3.4 27.3 1.0 OG D:SER393 3.6 54.5 1.0 OD1 D:ASN392 4.1 39.4 1.0 CB D:ASP361 4.3 25.8 1.0 ND2 D:ASN527 4.4 36.9 1.0 ND1 D:HIS528 4.4 32.0 1.0 CG D:HIS528 4.5 21.3 1.0 CB D:SER393 4.5 40.6 1.0 C3 D:FOC594 4.6 78.6 1.0 CE1 D:PHE359 4.6 22.4 1.0 CG D:GLU337 4.8 33.0 1.0 N D:SER393 4.8 40.8 1.0 O3 D:FOC594 4.9 88.3 1.0

Manganese binding site 5 out of 5 in the L-Fucose Isomerase From Escherichia Coli


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 5 of L-Fucose Isomerase From Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ E:Mn592 b:31.6 occ:1.00 O E:HOH657 2.2 33.3 1.0 NE2 E:HIS528 2.3 24.2 1.0 O1 E:FOC593 2.3 43.5 1.0 OD2 E:ASP361 2.5 39.3 1.0 O2 E:FOC593 2.5 77.7 1.0 OD1 E:ASP361 2.5 42.4 1.0 OE1 E:GLU337 2.8 36.1 1.0 CG E:ASP361 2.8 35.8 1.0 CE1 E:HIS528 3.2 24.5 1.0 CD2 E:HIS528 3.2 31.3 1.0 C1 E:FOC593 3.2 63.1 1.0 C2 E:FOC593 3.3 67.8 1.0 OE2 E:GLU337 3.4 25.6 1.0 CD E:GLU337 3.4 20.3 1.0 OG E:SER393 3.8 46.6 1.0 OD1 E:ASN392 4.2 33.5 1.0 ND2 E:ASN527 4.2 29.2 1.0 ND1 E:HIS528 4.2 24.3 1.0 CG E:HIS528 4.3 24.7 1.0 CB E:ASP361 4.3 31.4 1.0 CE1 E:PHE359 4.5 26.6 1.0 CB E:SER393 4.7 34.8 1.0 C3 E:FOC593 4.7 67.8 1.0 CG E:GLU337 4.9 19.4 1.0 N E:SER393 4.9 32.7 1.0 CA E:ASP361 5.0 25.7 1.0 O3 E:FOC593 5.0 69.6 1.0

J.E.Seemann, G.E.Schulz. Structure and Mechanism of L-Fucose Isomerase From Escherichia Coli. J.Mol.Biol. V. 273 256 1997.
ISSN: ISSN 0022-2836
PubMed: 9367760
DOI: 10.1006/JMBI.1997.1280