Manganese in PDB 1fpy: Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin

All present enzymatic activity of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin:
6.3.1.2;

The structure of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin, PDB code: 1fpy was solved by H.S.Gill, D.Eisenberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.89
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	230.600, 132.500, 195.900, 90.00, 102.40, 90.00
R / Rfree (%)	24.8 / 26.3

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 24;

Binding sites:

The binding sites of Manganese atom in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin (pdb code 1fpy). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 24 binding sites of Manganese where determined in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin, PDB code: 1fpy:
Jump to Manganese binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Manganese binding site 1 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn469 b:41.3 occ:1.00 OE1 A:GLU220 2.0 30.5 0.8 OE1 A:GLU131 2.3 19.8 1.0 OE1 A:GLU212 2.4 35.1 0.1 OEA A:PPQ5900 2.6 32.5 0.9 OE2 A:GLU212 2.9 36.9 0.1 CD A:GLU212 2.9 38.2 0.1 OE1 A:GLU212 2.9 32.9 0.8 CD A:GLU220 3.1 31.6 0.8 CD A:GLU131 3.4 58.9 1.0 OE2 A:GLU220 3.6 35.1 0.8 PDP A:PPQ5900 3.6 42.5 0.9 CD A:GLU212 3.8 32.8 0.8 CG A:GLU131 3.9 20.5 1.0 CEP A:PPQ5900 3.9 70.9 0.9 O A:HOH5915 3.9 35.5 0.4 CGP A:PPQ5900 4.1 16.9 0.9 CE1 A:HIS210 4.1 23.0 1.0 OE2 A:GLU212 4.1 34.9 0.8 CG A:GLU212 4.3 42.1 0.1 NE2 A:HIS210 4.4 36.9 1.0 CG A:GLU220 4.4 28.9 0.8 O3B A:ADP4471 4.5 99.4 0.7 OE2 A:GLU131 4.5 25.2 1.0 CB A:GLU220 4.6 26.6 0.8 CB A:GLU212 4.8 40.2 0.1 OEB A:PPQ5900 4.9 84.0 0.9 CG A:GLU212 4.9 30.6 0.8 CE1 A:HIS269 5.0 68.5 1.0 CBP A:PPQ5900 5.0 32.7 0.9

Manganese binding site 2 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn470 b:43.6 occ:1.00 OE2 A:GLU129 1.9 45.5 1.0 OE2 A:GLU357 2.2 56.1 1.0 O2B A:ADP4471 2.4 98.9 0.7 ND1 A:HIS269 2.4 34.9 1.0 O3B A:ADP4471 2.6 99.4 0.7 CD A:GLU129 2.9 63.5 1.0 PB A:ADP4471 3.0 0.0 0.7 OE1 A:GLU129 3.2 55.9 1.0 CD A:GLU357 3.3 56.0 1.0 CE1 A:HIS269 3.3 68.5 1.0 CG A:HIS269 3.4 57.7 1.0 OE1 A:GLU357 3.6 77.2 1.0 CEP A:PPQ5900 3.7 70.9 0.9 CB A:HIS269 3.8 37.6 1.0 NH2 A:ARG359 3.9 52.2 0.6 O1B A:ADP4471 4.1 0.0 0.7 CG A:GLU129 4.2 43.1 1.0 O3A A:ADP4471 4.3 0.0 0.7 NE2 A:HIS271 4.3 49.3 1.0 NE2 A:HIS269 4.4 37.2 1.0 CD2 A:HIS269 4.5 52.5 1.0 CG A:GLU357 4.6 52.8 1.0 CB A:GLU129 4.6 77.2 1.0 CZ A:ARG359 4.6 52.3 0.6 O A:HOH5915 4.7 35.5 0.4

Manganese binding site 3 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn469 b:41.3 occ:1.00 OE1 B:GLU220 2.0 30.5 0.8 OE1 B:GLU131 2.3 19.8 1.0 OE1 B:GLU212 2.4 35.1 0.1 OEA B:PPQ5901 2.6 32.5 0.9 OE2 B:GLU212 2.9 36.9 0.1 CD B:GLU212 2.9 38.2 0.1 OE1 B:GLU212 2.9 32.9 0.8 CD B:GLU220 3.1 31.6 0.8 CD B:GLU131 3.4 58.9 1.0 OE2 B:GLU220 3.6 35.1 0.8 PDP B:PPQ5901 3.6 42.5 0.9 CD B:GLU212 3.8 32.8 0.8 CG B:GLU131 3.9 20.5 1.0 CEP B:PPQ5901 3.9 70.9 0.9 O B:HOH5916 3.9 35.5 0.4 CGP B:PPQ5901 4.1 16.9 0.9 CE1 B:HIS210 4.1 23.0 1.0 OE2 B:GLU212 4.1 34.9 0.8 CG B:GLU212 4.3 42.1 0.1 NE2 B:HIS210 4.4 36.9 1.0 CG B:GLU220 4.4 28.9 0.8 O3B B:ADP4472 4.5 99.4 0.7 OE2 B:GLU131 4.5 25.2 1.0 CB B:GLU220 4.6 26.6 0.8 CB B:GLU212 4.8 40.2 0.1 OEB B:PPQ5901 4.9 84.0 0.9 CG B:GLU212 4.9 30.6 0.8 CE1 B:HIS269 5.0 68.5 1.0 CBP B:PPQ5901 5.0 32.7 0.9

Manganese binding site 4 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn470 b:43.6 occ:1.00 OE2 B:GLU129 1.9 45.5 1.0 OE2 B:GLU357 2.2 56.1 1.0 O2B B:ADP4472 2.4 98.9 0.7 ND1 B:HIS269 2.4 34.9 1.0 O3B B:ADP4472 2.6 99.4 0.7 CD B:GLU129 2.9 63.5 1.0 PB B:ADP4472 3.0 0.0 0.7 OE1 B:GLU129 3.2 55.9 1.0 CD B:GLU357 3.3 56.0 1.0 CE1 B:HIS269 3.3 68.5 1.0 CG B:HIS269 3.4 57.7 1.0 OE1 B:GLU357 3.6 77.2 1.0 CEP B:PPQ5901 3.7 70.9 0.9 CB B:HIS269 3.8 37.6 1.0 NH2 B:ARG359 3.9 52.2 0.6 O1B B:ADP4472 4.1 0.0 0.7 CG B:GLU129 4.2 43.1 1.0 O3A B:ADP4472 4.3 0.0 0.7 NE2 B:HIS271 4.3 49.3 1.0 NE2 B:HIS269 4.4 37.2 1.0 CD2 B:HIS269 4.5 52.5 1.0 CG B:GLU357 4.6 52.8 1.0 CB B:GLU129 4.6 77.2 1.0 CZ B:ARG359 4.6 52.3 0.6 O B:HOH5916 4.7 35.5 0.4

Manganese binding site 5 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 5 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn469 b:41.3 occ:1.00 OE1 C:GLU220 2.0 30.5 0.8 OE1 C:GLU131 2.3 19.8 1.0 OE1 C:GLU212 2.4 35.1 0.1 OEA C:PPQ5902 2.6 32.5 0.9 OE2 C:GLU212 2.9 36.9 0.1 CD C:GLU212 2.9 38.2 0.1 OE1 C:GLU212 2.9 32.9 0.8 CD C:GLU220 3.1 31.6 0.8 CD C:GLU131 3.4 58.9 1.0 OE2 C:GLU220 3.6 35.1 0.8 PDP C:PPQ5902 3.6 42.5 0.9 CD C:GLU212 3.8 32.8 0.8 CG C:GLU131 3.9 20.5 1.0 CEP C:PPQ5902 3.9 70.9 0.9 O C:HOH5916 3.9 35.5 0.4 CGP C:PPQ5902 4.1 16.9 0.9 CE1 C:HIS210 4.1 23.0 1.0 OE2 C:GLU212 4.1 34.9 0.8 CG C:GLU212 4.3 42.1 0.1 NE2 C:HIS210 4.4 36.9 1.0 CG C:GLU220 4.4 28.9 0.8 O3B C:ADP4473 4.5 99.4 0.7 OE2 C:GLU131 4.5 25.2 1.0 CB C:GLU220 4.6 26.6 0.8 CB C:GLU212 4.8 40.2 0.1 OEB C:PPQ5902 4.9 84.0 0.9 CG C:GLU212 4.9 30.6 0.8 CE1 C:HIS269 5.0 68.5 1.0 CBP C:PPQ5902 5.0 32.7 0.9

Manganese binding site 6 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 6 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn470 b:43.6 occ:1.00 OE2 C:GLU129 1.9 45.5 1.0 OE2 C:GLU357 2.2 56.1 1.0 O2B C:ADP4473 2.4 98.9 0.7 ND1 C:HIS269 2.4 34.9 1.0 O3B C:ADP4473 2.6 99.4 0.7 CD C:GLU129 2.9 63.5 1.0 PB C:ADP4473 3.0 0.0 0.7 OE1 C:GLU129 3.2 55.9 1.0 CD C:GLU357 3.3 56.0 1.0 CE1 C:HIS269 3.3 68.5 1.0 CG C:HIS269 3.4 57.7 1.0 OE1 C:GLU357 3.6 77.2 1.0 CEP C:PPQ5902 3.7 70.9 0.9 CB C:HIS269 3.8 37.6 1.0 NH2 C:ARG359 3.9 52.2 0.6 O1B C:ADP4473 4.1 0.0 0.7 CG C:GLU129 4.2 43.1 1.0 O3A C:ADP4473 4.3 0.0 0.7 NE2 C:HIS271 4.3 49.3 1.0 NE2 C:HIS269 4.4 37.2 1.0 CD2 C:HIS269 4.5 52.5 1.0 CG C:GLU357 4.6 52.8 1.0 CB C:GLU129 4.6 77.2 1.0 CZ C:ARG359 4.6 52.3 0.6 O C:HOH5916 4.7 35.5 0.4

Manganese binding site 7 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 7 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn469 b:41.3 occ:1.00 OE1 D:GLU220 2.0 30.5 0.8 OE1 D:GLU131 2.3 19.8 1.0 OE1 D:GLU212 2.4 35.1 0.1 OEA D:PPQ5903 2.6 32.5 0.9 OE2 D:GLU212 2.9 36.9 0.1 CD D:GLU212 2.9 38.2 0.1 OE1 D:GLU212 2.9 32.9 0.8 CD D:GLU220 3.1 31.6 0.8 CD D:GLU131 3.4 58.9 1.0 OE2 D:GLU220 3.6 35.1 0.8 PDP D:PPQ5903 3.6 42.5 0.9 CD D:GLU212 3.8 32.8 0.8 CEP D:PPQ5903 3.9 70.9 0.9 CG D:GLU131 3.9 20.5 1.0 O D:HOH5918 3.9 35.5 0.4 CGP D:PPQ5903 4.1 16.9 0.9 CE1 D:HIS210 4.1 23.0 1.0 OE2 D:GLU212 4.1 34.9 0.8 CG D:GLU212 4.3 42.1 0.1 NE2 D:HIS210 4.4 36.9 1.0 CG D:GLU220 4.4 28.9 0.8 O3B D:ADP4474 4.5 99.4 0.7 OE2 D:GLU131 4.5 25.2 1.0 CB D:GLU220 4.6 26.6 0.8 CB D:GLU212 4.8 40.2 0.1 OEB D:PPQ5903 4.9 84.0 0.9 CG D:GLU212 4.9 30.6 0.8 CE1 D:HIS269 5.0 68.5 1.0 CBP D:PPQ5903 5.0 32.7 0.9

Manganese binding site 8 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 8 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn470 b:43.6 occ:1.00 OE2 D:GLU129 1.9 45.5 1.0 OE2 D:GLU357 2.2 56.1 1.0 O2B D:ADP4474 2.4 98.9 0.7 ND1 D:HIS269 2.4 34.9 1.0 O3B D:ADP4474 2.6 99.4 0.7 CD D:GLU129 2.9 63.5 1.0 PB D:ADP4474 3.0 0.0 0.7 OE1 D:GLU129 3.2 55.9 1.0 CD D:GLU357 3.3 56.0 1.0 CE1 D:HIS269 3.3 68.5 1.0 CG D:HIS269 3.4 57.7 1.0 OE1 D:GLU357 3.6 77.2 1.0 CEP D:PPQ5903 3.7 70.9 0.9 CB D:HIS269 3.8 37.6 1.0 NH2 D:ARG359 3.9 52.2 0.6 O1B D:ADP4474 4.1 0.0 0.7 CG D:GLU129 4.2 43.1 1.0 O3A D:ADP4474 4.3 0.0 0.7 NE2 D:HIS271 4.3 49.3 1.0 NE2 D:HIS269 4.4 37.2 1.0 CD2 D:HIS269 4.5 52.5 1.0 CG D:GLU357 4.6 52.8 1.0 CB D:GLU129 4.6 77.2 1.0 CZ D:ARG359 4.6 52.3 0.6 O D:HOH5918 4.7 35.5 0.4

Manganese binding site 9 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 9 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ E:Mn469 b:41.3 occ:1.00 OE1 E:GLU220 2.0 30.5 0.8 OE1 E:GLU131 2.3 19.8 1.0 OE1 E:GLU212 2.4 35.1 0.1 OEA E:PPQ5904 2.6 32.5 0.9 OE2 E:GLU212 2.9 36.9 0.1 CD E:GLU212 2.9 38.2 0.1 OE1 E:GLU212 2.9 32.9 0.8 CD E:GLU220 3.1 31.6 0.8 CD E:GLU131 3.4 58.9 1.0 OE2 E:GLU220 3.6 35.1 0.8 PDP E:PPQ5904 3.6 42.5 0.9 CD E:GLU212 3.8 32.8 0.8 CG E:GLU131 3.9 20.5 1.0 CEP E:PPQ5904 3.9 70.9 0.9 O E:HOH629 3.9 35.5 0.4 CGP E:PPQ5904 4.1 16.9 0.9 CE1 E:HIS210 4.1 23.0 1.0 OE2 E:GLU212 4.1 34.9 0.8 CG E:GLU212 4.3 42.1 0.1 NE2 E:HIS210 4.4 36.9 1.0 CG E:GLU220 4.4 28.9 0.8 O3B E:ADP4475 4.5 99.4 0.7 OE2 E:GLU131 4.5 25.2 1.0 CB E:GLU220 4.6 26.6 0.8 CB E:GLU212 4.8 40.2 0.1 OEB E:PPQ5904 4.9 84.0 0.9 CG E:GLU212 4.9 30.6 0.8 CE1 E:HIS269 5.0 68.5 1.0 CBP E:PPQ5904 5.0 32.7 0.9

Manganese binding site 10 out of 24 in the Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 10 of Crystal Structure of Glutamine Synthetase From Salmonella Typhimurium with Inhibitor Phosphinothricin within 5.0Å range: probe atom residue distance (Å) B Occ E:Mn470 b:43.6 occ:1.00 OE2 E:GLU129 1.9 45.5 1.0 OE2 E:GLU357 2.2 56.1 1.0 O2B E:ADP4475 2.4 98.9 0.7 ND1 E:HIS269 2.4 34.9 1.0 O3B E:ADP4475 2.6 99.4 0.7 CD E:GLU129 2.9 63.5 1.0 PB E:ADP4475 3.0 0.0 0.7 OE1 E:GLU129 3.2 55.9 1.0 CD E:GLU357 3.3 56.0 1.0 CE1 E:HIS269 3.3 68.5 1.0 CG E:HIS269 3.4 57.7 1.0 OE1 E:GLU357 3.6 77.2 1.0 CEP E:PPQ5904 3.7 70.9 0.9 CB E:HIS269 3.8 37.6 1.0 NH2 E:ARG359 3.9 52.2 0.6 O1B E:ADP4475 4.1 0.0 0.7 CG E:GLU129 4.2 43.1 1.0 O3A E:ADP4475 4.3 0.0 0.7 NE2 E:HIS271 4.3 49.3 1.0 NE2 E:HIS269 4.4 37.2 1.0 CD2 E:HIS269 4.5 52.5 1.0 CG E:GLU357 4.6 52.8 1.0 CB E:GLU129 4.6 77.2 1.0 CZ E:ARG359 4.6 52.3 0.6 O E:HOH629 4.7 35.5 0.4

H.S.Gill, D.Eisenberg. The Crystal Structure of Phosphinothricin in the Active Site of Glutamine Synthetase Illuminates the Mechanism of Enzymatic Inhibition. Biochemistry V. 40 1903 2001.
ISSN: ISSN 0006-2960
PubMed: 11329256
DOI: 10.1021/BI002438H