Atomistry » Manganese » PDB 1ciw-1en5 » 1ecc
Atomistry »
  Manganese »
    PDB 1ciw-1en5 »
      1ecc »

Manganese in PDB 1ecc: Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine

Enzymatic activity of Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine

All present enzymatic activity of Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine:
2.4.2.14;

Protein crystallography data

The structure of Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine, PDB code: 1ecc was solved by J.M.Krahn, J.L.Smith, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.00 / 2.40
Space group P 43 21 2
Cell size a, b, c (Å), α, β, γ (°) 78.200, 78.200, 308.800, 90.00, 90.00, 90.00
R / Rfree (%) 17.6 / 26.5

Manganese Binding Sites:

The binding sites of Manganese atom in the Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine (pdb code 1ecc). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 5 binding sites of Manganese where determined in the Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine, PDB code: 1ecc:
Jump to Manganese binding site number: 1; 2; 3; 4; 5;

Manganese binding site 1 out of 5 in 1ecc

Go back to Manganese Binding Sites List in 1ecc
Manganese binding site 1 out of 5 in the Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn506

b:37.2
occ:1.00
 O2 A:PCP505 2.1 33.2 1.0
O3 A:PCP505 2.1 33.7 1.0
O1 A:PCP505 2.2 35.2 1.0
O A:HOH2378 2.2 31.6 1.0
O1B A:PCP505 2.2 33.4 1.0
O A:HOH2379 2.2 33.6 1.0
C2 A:PCP505 2.8 33.1 1.0
C1 A:PCP505 2.9 33.5 1.0
C3 A:PCP505 3.0 33.1 1.0
PB A:PCP505 3.3 37.5 1.0
OD1 A:ASP367 3.4 40.2 1.0
O3A A:PCP505 3.5 37.6 1.0
PA A:PCP505 3.5 35.5 1.0
OD1 A:ASP366 3.6 27.3 1.0
O A:HOH2411 3.7 39.1 1.0
C5 A:PCP505 3.8 32.3 1.0
O A:PRO302 4.0 29.1 1.0
C4 A:PCP505 4.0 33.3 1.0
O A:ILE301 4.2 25.7 1.0
O2B A:PCP505 4.2 34.9 1.0
O1A A:PCP505 4.3 33.8 1.0
OD2 A:ASP366 4.4 26.4 1.0
O3B A:PCP505 4.4 36.8 1.0
N A:GLU303 4.4 29.4 1.0
CG A:ASP366 4.4 28.2 1.0
CG A:ASP367 4.4 39.1 1.0
O2A A:PCP505 4.4 36.8 1.0
C A:PRO302 4.5 28.9 1.0
N A:THR304 4.5 31.2 1.0
CB A:THR304 4.6 27.6 1.0
CE1 A:TYR258 4.6 27.5 1.0
OG1 A:THR304 4.6 24.3 1.0
OD2 A:ASP367 4.7 37.7 1.0
CG2 A:ILE301 4.9 21.3 1.0

Manganese binding site 2 out of 5 in 1ecc

Go back to Manganese Binding Sites List in 1ecc
Manganese binding site 2 out of 5 in the Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn2376

b:42.4
occ:0.40
 OE2 A:GLU449 2.6 44.4 1.0
CD A:GLU449 3.2 44.8 1.0
OE1 A:GLU449 3.3 45.0 1.0
O A:ALA448 4.1 36.8 1.0
ND2 A:ASN277 4.5 23.2 1.0
CG A:GLU449 4.6 42.4 1.0
C A:ALA448 4.7 37.4 1.0
OD1 A:ASN277 4.7 24.2 1.0
CB A:ALA448 4.8 37.5 1.0

Manganese binding site 3 out of 5 in 1ecc

Go back to Manganese Binding Sites List in 1ecc
Manganese binding site 3 out of 5 in the Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn506

b:32.7
occ:1.00
 O3 B:PCP505 2.2 29.8 1.0
O2 B:PCP505 2.2 30.4 1.0
O1B B:PCP505 2.2 32.8 1.0
O B:HOH2452 2.3 30.3 1.0
O1 B:PCP505 2.3 32.5 1.0
O B:HOH2451 2.4 29.2 1.0
C2 B:PCP505 2.9 31.7 1.0
C3 B:PCP505 3.0 30.9 1.0
C1 B:PCP505 3.1 30.6 1.0
PB B:PCP505 3.4 37.8 1.0
OD1 B:ASP367 3.4 27.4 1.0
PA B:PCP505 3.5 34.9 1.0
O3A B:PCP505 3.5 35.4 1.0
OD1 B:ASP366 3.5 22.0 1.0
O B:HOH2460 3.8 19.6 1.0
C5 B:PCP505 3.9 30.3 1.0
C4 B:PCP505 4.0 30.9 1.0
OD2 B:ASP366 4.0 15.3 1.0
O B:PRO302 4.1 26.3 1.0
OG1 B:THR304 4.1 26.1 1.0
O B:ILE301 4.2 26.9 1.0
CG B:ASP366 4.2 16.8 1.0
O1A B:PCP505 4.2 33.4 1.0
CB B:THR304 4.3 23.0 1.0
O2B B:PCP505 4.3 35.4 1.0
N B:GLU303 4.5 29.1 1.0
O3B B:PCP505 4.5 34.1 1.0
O2A B:PCP505 4.5 34.1 1.0
C B:PRO302 4.5 27.3 1.0
N B:THR304 4.6 24.4 1.0
CG B:ASP367 4.6 25.7 1.0
CE1 B:TYR258 4.7 18.8 1.0
CG2 B:ILE301 4.9 17.6 1.0
OD2 B:ASP367 4.9 24.8 1.0
CD1 B:TYR258 5.0 18.9 1.0
CA B:THR304 5.0 23.5 1.0

Manganese binding site 4 out of 5 in 1ecc

Go back to Manganese Binding Sites List in 1ecc
Manganese binding site 4 out of 5 in the Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn1449

b:76.5
occ:0.40
 O B:ILE428 2.8 41.1 1.0
OE2 B:GLU376 3.0 62.9 1.0
CD B:GLU376 3.9 64.5 1.0
C B:ILE428 4.0 37.8 1.0
CG B:GLU376 4.2 58.6 1.0
C B:ILE429 4.7 37.5 1.0
OE2 B:GLU380 4.7 44.8 1.0
CA B:ILE429 4.8 36.6 1.0
O B:ILE429 4.9 40.9 1.0
N B:ILE429 4.9 38.0 1.0

Manganese binding site 5 out of 5 in 1ecc

Go back to Manganese Binding Sites List in 1ecc
Manganese binding site 5 out of 5 in the Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 5 of Escherichia Coli Glutamine Phosphoribosylpyrophosphate (Prpp) Amidotransferase Complexed with Mn-Cprpp and 5-Oxo- Norleucine within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn2449

b:32.3
occ:1.00
 OE2 B:GLU449 2.2 31.4 1.0
O B:HOH2543 2.4 33.6 1.0
O B:HOH2597 2.4 33.5 1.0
O B:HOH2600 2.6 32.2 1.0
O B:HOH2598 2.7 33.3 1.0
O B:HOH2599 2.7 31.7 1.0
CD B:GLU449 3.2 32.5 1.0
OE1 B:GLU449 3.4 33.7 1.0
OD1 B:ASN277 3.8 19.4 1.0
ND2 B:ASN277 4.1 19.9 1.0
CG B:ASN277 4.4 22.2 1.0
CB B:SER273 4.4 21.8 1.0
CG B:GLU449 4.6 29.1 1.0
OG B:SER273 4.8 24.4 1.0
O B:HOH2497 5.0 22.0 1.0

Reference:

J.M.Krahn, J.H.Kim, M.R.Burns, R.J.Parry, H.Zalkin, J.L.Smith. Coupled Formation of An Amidotransferase Interdomain Ammonia Channel and A Phosphoribosyltransferase Active Site. Biochemistry V. 36 11061 1997.
ISSN: ISSN 0006-2960
PubMed: 9333323
DOI: 10.1021/BI9714114
Page generated: Sat Oct 5 10:09:42 2024

Last articles

Zn in 9J0N
Zn in 9J0O
Zn in 9J0P
Zn in 9FJX
Zn in 9EKB
Zn in 9C0F
Zn in 9CAH
Zn in 9CH0
Zn in 9CH3
Zn in 9CH1
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy