Manganese in PDB 6bu5: Crystal Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter in the Outward-Open, Manganese-Bound Conformation

The structure of Crystal Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter in the Outward-Open, Manganese-Bound Conformation, PDB code: 6bu5 was solved by A.T.Bozzi, C.M.Zimanyi, J.M.Nicoludis, R.Gaudet, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.20 / 2.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	105.758, 80.389, 51.754, 90.00, 94.72, 90.00
R / Rfree (%)	23.7 / 27.9

The binding sites of Manganese atom in the Crystal Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter in the Outward-Open, Manganese-Bound Conformation (pdb code 6bu5). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter in the Outward-Open, Manganese-Bound Conformation, PDB code: 6bu5:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter in the Outward-Open, Manganese-Bound Conformation


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter in the Outward-Open, Manganese-Bound Conformation within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn501 b:79.5 occ:0.80 O A:ALA53 2.0 97.2 1.0 O A:HOH624 2.7 52.3 1.0 O A:HOH633 2.9 48.5 1.0 OD1 A:ASP56 2.9 72.3 1.0 OD2 A:ASP56 2.9 72.0 1.0 CG A:ASP56 3.0 64.1 1.0 SD A:MET230 3.0 71.5 1.0 C A:ALA53 3.2 75.9 1.0 OD1 A:ASN59 3.3 65.3 1.0 CA A:TYR54 3.5 54.5 1.0 CE A:MET230 3.6 71.3 1.0 CG A:MET230 3.7 65.6 1.0 N A:TYR54 3.7 63.8 1.0 C A:TYR54 4.0 50.5 1.0 CB A:ASP56 4.0 54.5 1.0 CG A:ASN59 4.3 58.1 1.0 N A:ASP56 4.3 53.8 1.0 CA A:ALA53 4.4 66.5 1.0 N A:MET55 4.4 54.7 1.0 O A:THR228 4.4 65.9 1.0 O A:TYR54 4.5 47.7 1.0 ND2 A:ASN59 4.6 59.7 1.0 CB A:TYR54 4.7 51.4 1.0 O A:HOH601 4.7 50.7 1.0 CA A:ASP56 4.8 55.8 1.0 CG A:TYR54 4.9 48.4 1.0 CB A:ALA53 4.9 67.7 1.0 CD1 A:TYR54 5.0 43.7 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter in the Outward-Open, Manganese-Bound Conformation


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Deinococcus Radiodurans Nramp/Mnth Divalent Transition Metal Transporter in the Outward-Open, Manganese-Bound Conformation within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn502 b:86.1 occ:0.50 O A:HOH629 2.2 54.3 1.0 NE2 A:HIS287 2.5 72.1 1.0 CD2 A:HIS287 3.3 68.9 1.0 CE1 A:HIS287 3.4 69.6 1.0 CG A:HIS287 4.4 63.9 1.0 ND1 A:HIS287 4.4 64.1 1.0

A.T.Bozzi, C.M.Zimanyi, J.M.Nicoludis, B.K.Lee, C.H.Zhang, R.Gaudet. Structures in Multiple Conformations Reveal Distinct Transition Metal and Proton Pathways in An Nramp Transporter. Elife V. 8 2019.
ISSN: ESSN 2050-084X
PubMed: 30714568
DOI: 10.7554/ELIFE.41124